Method for preparing probiotics leaven used for plant-derived albumen

A starter and probiotic technology, applied in the field of preparation of probiotic starter, can solve the problems of strict operating conditions, complex production process, high production cost, etc.

Inactive Publication Date: 2008-08-13
江苏云海辰龙生物科技有限公司 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there are domestic technologies for preparing polypeptide products containing lactic acid bacteria. For example, the patent application number 02156830.8 for secondary fermented soybean degraded yoghurt uses a two-step liquid fermentation method. The production process is complicated, the operating conditions are strict, and the production cost is relatively high.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] 1) Strain activation: The strain used is Lactobacillus plantarum (Lactobacillus plantarum) LP8, which has been published in the Journal of animal feed science and technology. The preserved strains were inserted into the slant of the MRS medium and cultured at 37°C until colonies appeared. Then pick the bacterial lawn from the inclined surface and connect it to another inclined surface. After the bacteria appear, repeat the above operation, and transfer 3 times in a row to obtain the activated bacterial strain. The composition of the MRS medium used was: peptone 10g / L; yeast extract 5g / L; diammonium citrate 2g / L; glucose 20g / L; beef extract 10g / L; Tween 80 1mL / L; 2 HPO 4 2g / L; MgSO 4 ·7H 2 O 0.58g / L; MnSO 4 4H 2 O 0.25g / L, agar 15-20g / L, pH 6.2-6.5, sterilized at 121°C for 15 minutes.

[0021] 2) Seed culture: inoculate the activated lactic acid bacteria strain obtained in step 1) into the liquid culture medium of the lactic acid bacteria triangular flask, and cult...

Embodiment 2

[0027] Steps 1 to 5 are the same as in Example 1, and the probiotic powder is mixed with acid protease and β-glucanase in a weight ratio of 10:80:10 to make a starter.

[0028] The use of this starter to ferment plant protein raw materials can not only degrade the macromolecular proteins in the raw materials into small peptides, but also eliminate trypsin inhibitors, flatulence factors and other components that are harmful to health in the raw materials, making the products more conducive to digestion and absorption. At the same time, the fermentation product contains a variety of organic acids, esters and other flavor substances and bacteriocins, which can decompose physiologically active substances such as isoflavones and phenols in the raw materials. The plant protein polypeptide rich in probiotics obtained by using the starter can be used for human health care and nutrition supplements, various livestock diets, fish and shrimp diets or other farmed animal diets. The type a...

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PUM

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Abstract

A method for preparation of living-benefiting bacterium leaven of plant source albumen belongs to biofermentation technology field. The process of the invention comprises culture selecting, activating, culturing, anticentripetal concentrating, drying and mixing with enzyme to produce composite leaven containing activity living-benefiting bacterium and enzyme. Adapting the leaven, macro molecule albumen in material is degraded into small peptides, the number of living-benefiting bacterium viable bacteria of fermentation producers stock is up to 10 million, the content of crude protein is increased by 3 to 5, the proteolysis degree is achieved 12 to 15, Ph value is reduced to 3.8 to 4.0, the content of lactic acid is 5 to 6 The fermentation producers can be used for human beings health care and nutrition tonic, various critters dialy feeder, fish and shrimp daily feeder or other breed aquatics animals daily feeder.

Description

technical field [0001] The invention relates to the technical field of biological fermentation, in particular to a preparation method of a probiotic starter for fermenting vegetable protein raw materials such as soybean meal, cottonseed meal and peanut meal. Background technique [0002] Probiotics are defined as microbial preparations or products that contain a sufficient number of live bacteria and have a defined composition, which can change the composition of the flora in a certain part of the host through colonization, thereby benefiting the health of the host. In addition to colonization, the metabolites of probiotics range from simple short-chain fatty acids to some essential and complex organic acids such as folic acid and orotic acid. These compounds can reduce the pH of the digestive tract and form an acidic environment, and regulate a series of physiological processes of the host. Various enzymes released by probiotics in the intestinal lumen can have a synergist...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12N9/50C12N9/42C12N9/46C12P21/06A23K1/18C12R1/25A23K10/12A23K10/37
CPCY02P60/87
Inventor 陈玉良李春阳闫征
Owner 江苏云海辰龙生物科技有限公司
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