Preparation and application of nylon affinity membrane with reactive blue 4 as ligand

A nylon membrane and affinity membrane technology, applied in the field of nylon affinity membrane, can solve the problems of complicated operation, unsatisfactory purification effect, low sample activity recovery rate, etc., and achieves simple operation, convenient large-scale extraction and purification, and convenient sample source. Effect
CN101259405BInactive Publication Date: 2010-06-02DONGHUA UNIV
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
DONGHUA UNIV
Publication Date
2010-06-02
Estimated Expiration
Not applicable · inactive patent

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Abstract

The invention relates to a preparing method and the application of a nylon affinity membrane which takes Reactive Blue 4 as a petunidin. The preparation includes: the nylon membrane after being activated is modified by chitosan; the modified nylon membrane is dipped into a Reactive Blue 4 dye and added with NaCL solution after heat preservation to react, added with Na2CO3 solution for reacting continuously after temperature rising, respectively washed by hot de-ionized water, methanol solution, NaCl solution, urea solution and de-ionized water to obtain the nylon affinity membrane which takesReactive Blue 4 as a petunidin. The application includes that pawpaw powders are sampled on the nylon affinity membrane which takes Reactive Blue 4 as a petunidin, washed by Tris-HCL buffer solution and eluted by NaSCN solution to obtain pawpaw prolease; the activity of the prolease and the protein content are tested; the purification times are calculated. The method is fast, simple and convenientand has large separation capacity; the prolease extracted has the advantages of good activity, high purity as well as being suitable for scale production.
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Description

technical field

[0001] The invention belongs to the field of nylon affinity membranes, in particular to a preparation method and application of a nylon affinity membrane with Reactive Blue 4 as ligand. Background technique

[0002] Papain (EC3.4.22.2) is a kind of sulfhydryl protease, which widely exists in the root, stem, leaf and fruit of papaya (Carica papaya), and is most abundant in immature milk. Widely used in food industry, such as clarification of beer and tenderization of meat, as well as leather, textile, daily chemical and pharmaceutical industries. The purification of papain mostly adopts the precipitation method, but this method is still mixed with other proteases, which cannot meet the needs of the pharmaceutical industry.

[0003] At present, the methods for separating and purifying papain include rough separation and refined separation, wherein rough separation includes salting out method, isoelectric point precipitation method, organic solvent fractionatio...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
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