Inducement and preparation of S. keratinase and method for sorting wool by using the same
What is Al technical title?
Al technical title is built by PatSnap Al team. It summarizes the technical point description of the patent document.
A technology of keratinase and wool, which is applied in the field of S. keratinase induction, production and use to finish wool, can solve the problems of unreached, discounted industrial production prospects, etc., achieve mechanical strength improvement, easy industrialization promotion, and save treatment The effect of steps
Inactive Publication Date: 2008-09-17
DONGHUA UNIV
View PDF0 Cites 7 Cited by
Summary
Abstract
Description
Claims
Application Information
AI Technical Summary
This helps you quickly interpret patents by identifying the three key elements:
Problems solved by technology
Method used
Benefits of technology
Problems solved by technology
In this way, the original purpose of using biological enzymes is not only not achieved, but one more step is add
Method used
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more
Image
Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
Click on the blue label to locate the original text in one second.
Reading with bidirectional positioning of images and text.
Smart Image
Examples
Experimental program
Comparison scheme
Effect test
Embodiment 1
[0025] Using Stenotrophomonas maltophilia DHHJ as the original strain and using wool as the inducer, the optimal culture conditions were determined through orthogonal experiments, and its induction and domestication culture: wool 2g, sodium chloride 0.4g , 0.4 g of disodium hydrogen phosphate, 0.03 g of potassium dihydrogen phosphate, 100 ml of water, pH=7.5, 40° C., constant temperature culture for 2 to 3 days.
[0026] After several times of domestication, high-efficiency and stable strains were obtained, and the following culture ratios were used for amplified culture: chicken feather 2g, sodium chloride 0.4g, disodium hydrogen phosphate 0.4g, potassium dihydrogen phosphate 0.03g, water 100ml, pH= 7.5, 40° C., constant temperature culture for 72 hours to obtain enzyme-containing culture solution.
Embodiment 2
[0028] After centrifugation, salting-out, standing, re-centrifugation, purification and concentration of the obtained enzyme-containing culture solution, the wool is treated and detected by a 280nm ultraviolet spectrophotometer and a microscope to obtain the optimal treatment conditions: enzyme concentration 3% (owf), pH=7.8, treated at 40°C for 75min.
[0029] Measure parameters such as weight reduction rate, felt shrinkage rate, alkali solubility, strength, whiteness, etc., to prove its macroscopic treatment effect on wool.
Embodiment 3
[0031] After the wool fiber was treated with S. keratinase, the changes of weight reduction rate, felt shrinkage rate, alkali solubility, strength and whiteness were analyzed. Discuss the mode of action of S. keratinase on wool, the influence coefficient on fiber damage and fabric strength and the feasibility of large-scale application.
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more
PUM
Login to view more
Abstract
The invention relates to a method for inducing and preparing an S. keratinase and a method for processing wool. The method for preparing the S. keratinase comprises the steps of using Stenotrophomonas maltophilia DHHJ as the original strain; culturing for 2-4 days as a generation at the constant temperature of 30-50DEG C when the pH value is equal to 7.5 by using 1-3g of wool, 0.2-1g of sodium chloride, 0.2-1g of disodium hydrogen phosphate, 0.01-0.1g of monopotassium phosphate and 50-200ml of water; continuously inducing and domesticating; culturing for 2-4days at the constant temperature of 30-50 DEG C when the pH value is equal to 7.5 by using 1-3g of chicken feather meal (or other feather meal), 0.2-1g of sodium chloride, 0.2-1g of disodium hydrogen phosphate, 0.01-0.1g of monopotassium phosphate and 50-200ml of water after efficient and stable strains are obtained so as to obtain enzyme culture solution; subjecting the culture solution to centrifugation, salting out, standing, centrifugation and purification to obtain the S. keratinase. The wool processing technique comprises the step of processing the wool and the textile thereof for 60-120min by using purified and concentrated S. keratinase with the pH value being equal to 7.8, the temperature being 20-50 DEG C, and enzyme concentration being 2-5% (owf). The S. keratinase can directly act on the wool so as to eliminate the chemical agent processing step, and be used for wool shrink-resistant processing with more environmental friendliness and high efficiency. The processed wool with enzyme has the same characteristics as cashmere in many aspects, and can be used for the processing and the production of cashmere imitation products.
Description
technical field [0001] The invention belongs to the field of microbial domestication, enzyme preparation and wool finishing methods, and in particular relates to a method for inducing and preparing S. keratinase and using it to finish wool Background technique [0002] Wool and its fabrics can improve their felting resistance through finishing, and can also improve and improve the luster, whiteness and hand feeling of wool, and reduce the tingling sensation to human skin. [0003] At present, the anti-felting of wool mostly adopts chlorinated-resin process, which can effectively improve the anti-felting performance and wearing comfort of wool. It is a relatively mature process, but it is worth noting that it produces absorbability. The carcinogenicity and non-biodegradability of halide AOX are very harmful to the environment: 350g of AOX will be produced when 1t of wool is treated with chlorination-resin process. Today, as all human beings pay more and more attention to env...
Claims
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more
Application Information
Patent Timeline
Application Date:The date an application was filed.
Publication Date:The date a patent or application was officially published.
First Publication Date:The earliest publication date of a patent with the same application number.
Issue Date:Publication date of the patent grant document.
PCT Entry Date:The Entry date of PCT National Phase.
Estimated Expiry Date:The statutory expiry date of a patent right according to the Patent Law, and it is the longest term of protection that the patent right can achieve without the termination of the patent right due to other reasons(Term extension factor has been taken into account ).
Invalid Date:Actual expiry date is based on effective date or publication date of legal transaction data of invalid patent.
Login to view more 
Login to view more