Inducement and preparation of S. keratinase and method for sorting wool by using the same

Abstract

The invention relates to a method for inducing and preparing an S. keratinase and a method for processing wool. The method for preparing the S. keratinase comprises the steps of using Stenotrophomonas maltophilia DHHJ as the original strain; culturing for 2-4 days as a generation at the constant temperature of 30-50DEG C when the pH value is equal to 7.5 by using 1-3g of wool, 0.2-1g of sodium chloride, 0.2-1g of disodium hydrogen phosphate, 0.01-0.1g of monopotassium phosphate and 50-200ml of water; continuously inducing and domesticating; culturing for 2-4days at the constant temperature of 30-50 DEG C when the pH value is equal to 7.5 by using 1-3g of chicken feather meal (or other feather meal), 0.2-1g of sodium chloride, 0.2-1g of disodium hydrogen phosphate, 0.01-0.1g of monopotassium phosphate and 50-200ml of water after efficient and stable strains are obtained so as to obtain enzyme culture solution; subjecting the culture solution to centrifugation, salting out, standing, centrifugation and purification to obtain the S. keratinase. The wool processing technique comprises the step of processing the wool and the textile thereof for 60-120min by using purified and concentrated S. keratinase with the pH value being equal to 7.8, the temperature being 20-50 DEG C, and enzyme concentration being 2-5% (owf). The S. keratinase can directly act on the wool so as to eliminate the chemical agent processing step, and be used for wool shrink-resistant processing with more environmental friendliness and high efficiency. The processed wool with enzyme has the same characteristics as cashmere in many aspects, and can be used for the processing and the production of cashmere imitation products.

Description

technical field [0001] The invention belongs to the field of microbial domestication, enzyme preparation and wool finishing methods, and in particular relates to a method for inducing and preparing S. keratinase and using it to finish wool Background technique [0002] Wool and its fabrics can improve their felting resistance through finishing, and can also improve and improve the luster, whiteness and hand feeling of wool, and reduce the tingling sensation to human skin. [0003] At present, the anti-felting of wool mostly adopts chlorinated-resin process, which can effectively improve the anti-felting performance and wearing comfort of wool. It is a relatively mature process, but it is worth noting that it produces absorbability. The carcinogenicity and non-biodegradability of halide AOX are very harmful to the environment: 350g of AOX will be produced when 1t of wool is treated with chlorination-resin process. Today, as all human beings pay more and more attention to env...

AI Technical Summary

Problems solved by technology

In this way, the original purpose of using biological enzymes is not only not achieved, but one more step is added compared with the chlorination-resin method, and the prospect of its industrial production is greatly reduced.

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