Collagen bioactive peptide, preparation and use thereof

Abstract

The present invention provides a collagen peptide prepared by biochemical technology preparing method, including using underatured collagen come from aquatic animals, especially underatured I type collagen as raw material, then obtaining the collagen peptide by enzymatic hydrolysing, dialysing, drying in a medium temperature conditions. The molecular weight range of the main component of the prepared collagen peptide is 5-13 KD, the index of soluble nitrogen is more than 98%, the collagen peptide has specially physiological functions of prolonging animal life and inhibiting inner lipofuscin generation, widely used for health food and pet health feed additive as functional factor additive.

Description

technical field [0001] The invention belongs to the technical field of active peptide development, and specifically relates to a collagen with special physiological effects such as prolonging animal life and inhibiting the formation of brown lipids, which is obtained from undenatured collagen derived from aquatic animals through special biochemical techniques such as enzymolysis. Protein active peptide and its preparation method. Background technique [0002] Active peptides refer to peptides that can regulate the metabolism of organisms or have some special physiological activities. , antibacterial, antiviral, anticoagulant, blood pressure lowering, etc. Collagen is a major structural protein that widely exists between animal cells. Its uses include food, beauty, biomedical materials for external use and implantation, etc. It has become an important active molecule. The hydrolyzed collagen product contains a variety of active peptides with various physiological functions,...

AI Technical Summary

Problems solved by technology

Among them, CN1233840C relates to a collagen polypeptide manufacturing method and its products, CN1404748A relates to protein peptide health food and its preparation method, CN1994133A relates to the application method of collagen peptide in food, and CN1552889A relates to a method of preparing collagen and collagen polypeptide and its application , but these patents all use collagen as raw material, do not involve aquatic products, and have no reports on the physical and chemical characteristics and biological activities of collagen peptides; The production process and method of preparing collagen peptides from starfish shells, but the biological activities of these collagen peptides are not reported in detail in these patents; CN101091789A relates to the use of marine collagen peptides in the preparation of immunomodulatory drugs or food, obtained from fish skin by hydrolysis Small molecule peptides with a molecular weight of 200-1000D can enhance the ability of delayed allergic reactions in mice and the proliferation of T lymphocytes stimulated by ConA, increase the number of antibody-producing cells, and clear the carbon particles of macrophages and chicken red blood cells. The phagocytosis ability has obvious promoting effect; CN101061827A relates to the preparation method and application of squid skin collagen active peptide, which is characterized in that collagen is extracted with hot water, then hydrolyzed by adding enzyme at 40-50°C, inactivating enzyme at 90-100°C, and using alkali The pH of the solution was adjusted to neutral, the enzymatic solution was filtered, ultrafiltered, sodium filtered, vacuum concentrated and spray-dried. The obtained collagen peptides had anti-oxidation, blood pressure lowering, anti-atherosclerosis and cosmetic effects, but no Collagen peptide has the effect of prolonging the life span of animals and inhibiting the formation of brown lipids in animals.