Collagen bioactive peptide, preparation and use thereof

A collagen peptide and collagen technology, which is applied in food preparation, application, animal feed, etc., can solve the problems of not reporting the biological activity of collagen peptides in detail, not seeing the physical and chemical characteristics of collagen peptides, and increasing the number of antibody-producing cells, etc. Achieve the effect of inhibiting the activity of polyphenol oxidase in vitro, high yield and high food safety

Active Publication Date: 2008-10-01
SHANGHAI OCEAN UNIV
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  • Summary
  • Abstract
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  • Application Information

AI Technical Summary

Problems solved by technology

Among them, CN1233840C relates to a collagen polypeptide manufacturing method and its products, CN1404748A relates to protein peptide health food and its preparation method, CN1994133A relates to the application method of collagen peptide in food, and CN1552889A relates to a method of preparing collagen and collagen polypeptide and its application , but these patents all use collagen as raw material, do not involve aquatic products, and have no reports on the physical and chemical characteristics and biological activities of collagen peptides; The production process and method of preparing collagen peptides from starfish shells, but the biological activities of these collagen peptides are not reported in detail in these patents; CN101091789A relates to the use of marine collagen peptides in the preparation of immunomodulatory drugs or food, obtained from fish skin by hydrolysis Small molecule peptides with a molecular weight of 200-1000D can enhanc

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Example Embodiment

[0028] Example 1

[0029] (1) The washed fish skin is mashed, and then treated with 5-15 times of purified water and 5% NaCl solution to remove water-soluble and salt-soluble impurities respectively.

[0030] (2) Treat the fish skin from which impurities have been removed with 20 times 1% pepsin solution (pH2) for 72 hours, centrifuge (5000×g), take the supernatant, add solid NaCl to make the concentration reach 2.5M, centrifuge (5000×g) ×g), take the precipitate to obtain crude collagen.

[0031] (3) Dissolve the crude collagen with 0.5M acetic acid, and use 0.5M acetic acid as the external dialysis fluid for dialysis for 48-72h, until the dialysate is free of Cl - Until detection, undenatured type I collagen has been obtained.

[0032] (4) Disperse collagen in 20 times the amount of pure water, adjust the pH to 8, heat to 50°C, keep for 40 minutes, and then add trypsin (enzyme and substrate concentration of 60 U / g) in proportion to enzymatic hydrolysis 20mins or until the degree...

Example Embodiment

[0035] Example 2

[0036]Disperse the undenatured type I collagen obtained in steps (1), (2), and (3) in Example 1 in 10 times the amount of pure water, adjust the pH to 2, and heat to 60°C, according to the example Add pepsin in the ratio of 1 for enzymatic hydrolysis. The enzymatic hydrolysis time is about 40mins or until the degree of hydrolysis reaches 15%; put the collagen hydrolysate into a dialysis bag with a molecular weight cut-off of 2kD, and conduct dialysis overnight with distilled water as the external fluid. After freeze-drying to obtain collagen active peptide, after separation and purification by Sephadex-G50 chromatography, the molecular weight of the obtained effective component is 5021D.

Example Embodiment

[0037] Example 3

[0038] Disperse the undenatured type I collagen obtained in steps (1), (2), (3) in Example 1 in 10 times the amount of pure water, adjust the pH to 2, and add in the proportions in Example 1. Pepsin is used for enzymolysis, the temperature is controlled at 30℃, and the enzymolysis time is about 40mins or until the degree of hydrolysis reaches 5%; put the collagen hydrolysate into a dialysis bag with a molecular weight cut-off of 2kD, and conduct dialysis overnight with distilled water as the outer fluid , And then freeze-dried to obtain the collagen active peptide, and after separation and purification by Sephadex-G50 chromatography, the molecular weight of the obtained effective component is 5021D.

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Abstract

The present invention provides a collagen peptide prepared by biochemical technology preparing method, including using underatured collagen come from aquatic animals, especially underatured I type collagen as raw material, then obtaining the collagen peptide by enzymatic hydrolysing, dialysing, drying in a medium temperature conditions. The molecular weight range of the main component of the prepared collagen peptide is 5-13 KD, the index of soluble nitrogen is more than 98%, the collagen peptide has specially physiological functions of prolonging animal life and inhibiting inner lipofuscin generation, widely used for health food and pet health feed additive as functional factor additive.

Description

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Claims

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Application Information

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Owner SHANGHAI OCEAN UNIV
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