Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Iota-carrageenan even oligosacchride monomer and preparation thereof

A technology of even-numbered and even-numbered oligosaccharides of carrageenan, applied in chemical instruments and methods, oligosaccharides, sugar derivatives, etc., to achieve the effects of no three waste pollution, simple preparation process, and high preparation efficiency

Inactive Publication Date: 2008-10-08
OCEAN UNIV OF CHINA
View PDF3 Cites 6 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although there are patent reports on the preparation of K-carrageenan odd-numbered oligosaccharide monomers (Yu Guangli, etc., ZL03138968.6) and agar gum odd-numbered oligosaccharide monomers (Mao Wenjun, etc., ZL03138971.6) by acid degradation, there is no such thing as yet. A report on preparation of iota-carrageenan even-numbered oligosaccharide monomer by acid degradation

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Iota-carrageenan even oligosacchride monomer and preparation thereof
  • Iota-carrageenan even oligosacchride monomer and preparation thereof
  • Iota-carrageenan even oligosacchride monomer and preparation thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0013] Dissolve iota-carrageenan into a 0.5-5% (W / W) aqueous solution with sulfuric acid at a concentration of 0.1-1.0mol / L, and degrade the aqueous solution at 50-100°C for 30-480 minutes under stirring, Neutralize with sodium hydroxide, concentrate by rotary evaporation, precipitate and wash with absolute ethanol, dissolve the precipitate in water, then use 0.2mol / L ammonium bicarbonate aqueous solution as the mobile phase, and use Superdex 30 packing for column chromatography. Detected by a differential detector, combined and collected according to the elution peaks, concentrated, and freeze-dried to obtain the iota-carrageenan even-numbered oligosaccharide monomer of the present invention.

[0014] The sulfuric acid described in the present embodiment can use hydrochloric acid, nitric acid, formic acid, acetic acid, oxalic acid, citric acid or trifluoroacetic acid instead; Described sodium hydroxide can use potassium hydroxide or ammonium hydroxide instead; Described ethano...

Embodiment 2

[0016] Dissolve iota-carrageenan in water into a 0.5-5% (W / W) aqueous solution, add 0.5-20 grams of hydrogen-type strong cation exchange resin, and degrade the above mixed solution at 50-100°C for 30-480°C under stirring Minutes later, the filtrate was neutralized with ammonium hydroxide, concentrated by rotary evaporation, precipitated with acetone, washed, dissolved in water, and then separated by BioGel P10 column chromatography with 0.3 mol / L ammonium bicarbonate aqueous solution as mobile phase , detected with a differential detector, combined and collected according to the elution peaks, concentrated, and freeze-dried to obtain the product.

[0017] The hydrogen-type strong cation exchange resin described in this embodiment refers to 732 resin, Dowex AG50W X4 resin or Amberlite IR-120H resin; the acetone can be replaced by ethanol, methanol or isopropanol; the BioGel P10 used can be replaced by Superdex 30; described ammonium hydroxide can use sodium hydroxide or potassi...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

Disclosed is an even oligosaccharide alcohol monomer of iota-carrageenan, with polymerization degree at 2-20 and molecular formula as C12n+12H16n+18O15n+16S2n+2Na2n+2 (n=0-9). The preparation includes the following steps: heating and degrading iota-carrageenan with acid or hydro-strong cation resin under 50-100 DEG C for 30-480min; filtrating and then neutralizing the filtrate through alkaline; condensing; sedimenting through alcohol; proceeding Superdex 30 or BioGel P10 chromatographic column separation, with aqueous ammonium bicarbonate solution as mobile phase; detecting through a differnetial detector; combining and collecting according to leaching peak; condensing; freezing and drying. The method is covenient in operation, simple in process, low in cost and high in efficiency, and causes no pollution(gas, liquid and solid).

Description

technical field [0001] The invention relates to an iota-carrageenan oligosaccharide, in particular to an iota-carrageenan even-numbered oligosaccharide monomer with a polymerization degree of 2-20 and a preparation method thereof. Background technique [0002] Carrageenan is a kind of polyanionic linear polymer compound composed of galactose and galactose derivatives. Carrageenan mainly has three types: kappa, lambda and iota, among which iota-carrageenan is composed of 1,3-linked-4-sulfate-β-D-galactose (G4S) and 1,4-linked-2-sulfuric acid A high molecular polymer formed by alternately linking 3,6-inner ether-α-D-galactose (A2S). As a natural sulfated polysaccharide compound, carrageenan has been widely used in the fields of food, medicine and chemical industry. In recent years, many important biological functions and physiological activities of carrageenan oligosaccharides and their derivatives have been gradually recognized by people, and have been widely used in antitu...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): C07H11/00C07H3/06C08B37/00
Inventor 于广利杨波赵峡焦广玲郝翠王皓
Owner OCEAN UNIV OF CHINA
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com