Screening method for low-oxygen-consumption producer strain of clavulanic acid
A technology for producing clavulanic acid and bacterial strains, which is applied in the field of biomedicine, and can solve problems such as the influence of electric energy, the consumption of stirring speed and ventilation volume, and the consumption of large electric energy, and achieve the effects of low cost, reduced stirring speed and ventilation volume, and reduced electricity consumption
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Embodiment 1
[0013] Mutagenesis: Streptomyces clavuligerus spore suspensions were mutagenized by NTG.
[0014] Preliminary screening: Prepare 3.5L of plate medium according to the following formula: glucose 8g / L, malt extract 6g / L, yeast extract 4g / L, agar 25g / L, pH 7.2. Prepare 100 sterile Petri dishes. After the culture medium is sterilized, it is poured into a Petri dish to make a flat plate. Appropriately dilute the mutagenized spore suspension and spread it on a plate, and cultivate it in a tri-gas incubator with an oxygen concentration of 16%, and cultivate it at 28° C. for 10-12 days. Prepare 4L of slant medium according to the following formula: glucose 8g / L, malt extract 6g / L, yeast extract 4g / L, agar 25g / L, pH 7.2. Prepare 200 test tubes and make inclined planes for later use. Select the strains with good growth and spore production on the plate to inoculate the slant, cultivate in a three-gas incubator with an oxygen concentration of 16%, and cultivate at 28°C for 10-12 days....
Embodiment 2
[0018] Mutagenesis: Streptomyces clavuligerus spore suspensions were mutagenized by ultraviolet light.
[0019] Preliminary screening: Prepare 3.5L of plate medium according to the following formula: glucose 8g / L, malt extract 5g / L, yeast extract 4g / L, agar 24g / L, pH 7.2. Prepare 100 sterile Petri dishes. After the culture medium is sterilized, it is poured into a Petri dish to make a flat plate. Appropriately dilute the mutagenized spore suspension and smear it on a plate, and cultivate it in a tri-gas incubator with an oxygen concentration of 18%, and cultivate it at 28° C. for 10-12 days. Prepare 4L of slant medium according to the following formula: glucose 8g / L, malt extract 5g / L, yeast extract 4g / L, agar 25g / L, pH 7.2. Prepare 200 test tubes and make inclined planes for later use. Select the strains with good growth and spore production on the plate to inoculate the slant, cultivate in a three-gas incubator with an oxygen concentration of 18%, and cultivate at 28°C fo...
Embodiment 3
[0023] Mutagenesis: Streptomyces clavuligerus spore suspensions were mutagenized by microwaves.
[0024] Preliminary screening: Prepare 3.5L of plate medium according to the following formula: glucose 8g / L, malt extract 6g / L, yeast extract 4g / L, agar 25g / L, pH 7.2. Prepare 100 sterile Petri dishes. After the culture medium is sterilized, it is poured into a Petri dish to make a flat plate. Appropriately dilute the mutagenized spore suspension and spread it on a plate, and cultivate it in a tri-gas incubator with an oxygen concentration of 16%, and cultivate it at 28° C. for 10-12 days. Prepare 4L of slant medium according to the following formula: glucose 8g / L, malt extract 6g / L, yeast extract 4g / L, agar 25g / L, pH 7.2. Prepare 200 test tubes and make inclined planes for later use. Select the strains with good growth and spore production on the plate to inoculate the slant, cultivate in a three-gas incubator with an oxygen concentration of 17%, and cultivate at 28°C for 10-1...
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