Preparation and use of lilac grey streptomycete and active product thereof
A technology of Streptomyces lavender and Streptomyces, applied in botany equipment and methods, microbial-based methods, biochemical equipment and methods, etc., can solve the problems that the potential has not been fully developed and applied, and achieve low cost , easy to operate, good effect
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Embodiment 1
[0034] Embodiment one: Screening and identification of bacterial strain xjy
[0035] Through the antibacterial test in the dish, 6 strains were screened out from 51 strains of soil actinomycetes to have inhibitory activity against tomato leaf mold (Fulviafulva), among which strain xjy had the best inhibitory effect. The antimicrobial spectrum of strain xjy was measured and found that its sterile fermentation filtrate had different degrees of inhibitory effect on 23 common plant pathogenic fungi and 6 kinds of bacteria ( figure 1 , A: tomato leaf mold B: wheat sheath blight C: kiwifruit dry blight D: pear rot fungus. ), and the bacterial strain xjy has good subculture antibacterial stability, and can produce β-1,3-glucanase and protease; through the morphological characteristics of the bacterial strain xjy ( figure 2 A: spore; B: hyphae), cultural characteristics, physiological and biochemical characteristics, analysis of cell wall components and 16S rDNA homology sequence an...
Embodiment 2
[0036] Embodiment two: the preparation method of bacterial strain xjy antibacterial active substance
[0037]First, vibrate on the seed medium (5g peptone per liter, 3g yeast extract, 2g glucose, 1g beef extract, pH7.0, liquid volume 40mL / 250mL) at 28°C, 80r / min for 2 days to make seed liquid; then Add 10% seed liquid in the fermentation medium (every liter contains 2% soybean powder, 2% glucose, 0.8% NaCl, CaCO 3 0.2%, (NH 4 ) 2 SO 4 0.32%, pH 7.0, liquid volume 40mL / 250mL) at 28°C, 180r / mim shake flask culture for 6 days, the fermentation broth was centrifuged twice at 13000rpm for 20min each time to obtain the sterile supernatant.
Embodiment 3
[0038] Embodiment three: the property and structural analysis of the active substance produced by bacterial strain xjy
[0039] Through the stable determination of the sterile supernatant after the fermentation of bacterial strain xjy, it was found that the active substance produced by bacterial strain xjy had better thermal stability ( image 3 , ①A: 50°C 30min; ①B: 50°C 60min; ①C: 50°C 90min; ①D: 50°C 120min; ①E: 50°C 150min; ①F: sterile supernatant; ; C: 90min at 100°C; D: 120min at 100°C; E: 150min at 100°C; F: sterile supernatant), acid-base stability and light UV stability, which are alkaline water-soluble substances; the fermentation of xjy strain Use oxalic acid to adjust the pH value of the sterile supernatant to 2 and precipitate overnight. After suction filtration, use an equal volume of acetone to incubate in a water bath at 50°C for 1 hour. After the acetone is removed by filtration and concentration, the pH is adjusted to 7, and the pretreated antibacterial activ...
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