Method for converting podophyllinic acid lactone into podophyllic acid and picropodophyllin
A technology for podophyllotoxin and picropodophyllin, which is applied in biochemical equipment and methods, microorganism-based methods, organic chemistry and other directions, can solve the problems of easily polluted environment and low product yield, and achieves environmental friendliness and product yield. The effect of high rate and mild reaction conditions
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Embodiment 1
[0060] 1. Cultivation of Pseudomonas aeruginosa:
[0061] Slope strain culture: Medium: glucose 2%, beef extract 0.3%, peptone 0.5%, sodium chloride 0.5%, agar 2%, pH 7, sterilized at 115 degrees Celsius for 30 minutes, cooled and prepared after sterilization On the slope, inoculate Pseudomonas aeruginosa strains and incubate at 37°C for 4 hours;
[0062] Liquid seed culture: Medium: yeast powder 0.5%, peptone 0.5%, sodium chloride 0.5%, sucrose 1%, pH value 7, filling volume 50ml / 250ml shake flask, sterilization at 115°C for 30 minutes, sterilization Cool after the bacteria, inoculate the slant strain, culture at 37 degrees Celsius, 200 rpm for 3 hours, as liquid seeds;
[0063] Fermentation culture: Medium: yeast powder 0.5%, peptone 0.5%, sodium chloride 0.5%, sucrose 1%, pH value 7, liquid volume 50ml / 250ml shake flask, sterilized at 115°C for 30 minutes, sterilized After cooling, liquid seeds were inoculated with an inoculum amount of 10%, 37 degrees Celsius, 200 revolutions / m...
Embodiment 2
[0070] 1. Cultivation of Bacillus:
[0071] Slope strain culture: medium: beef extract 0.3%, yeast extract 0.5%, peptone 0.5%, agar 1.5%, pH 7, sterilized at 115 degrees Celsius for 30 minutes, cooled after sterilization, made a slant, inoculated with Bacillus Strains, cultured at 28 degrees Celsius for 12 hours;
[0072] Liquid seed culture: medium: beef extract 0.3%, yeast extract 0.5%, peptone 0.5%, pH 7, filling volume 50ml / 250ml shake flask, sterilized at 115°C for 30 minutes, cooled and inoculated after sterilization Inclined strains, cultured at 28 degrees Celsius, 180 revolutions / min for 10 hours, as liquid seeds;
[0073] Fermentation culture: medium: beef extract 0.3%, yeast extract 0.5%, peptone 0.5%, pH 7, filling volume 50ml / 250ml shake flask, sterilizing at 115°C for 30 minutes, cooling and inoculating liquid after sterilization Seeds, inoculum amount 10%, 28 degrees Celsius, 180 revolutions / min, culture for 10 hours.
[0074] 2. Biotransformation
[0075] The podophyll...
Embodiment 3
[0079] 1. Cultivation of Rhodococcus erythropolis:
[0080] Slope strain culture: medium: beef extract 0.3%, peptone 1.0%, sodium chloride 0.5%, agar 1.5%, pH 7, sterilized at 115 degrees Celsius for 30 minutes, cooled after sterilization, made a slant, inoculated with red Rhodococcus strains, cultivated at 30 degrees Celsius for 12 hours;
[0081] Liquid seed culture: Medium: beef extract 0.3%, peptone 1.0%, sodium chloride 0.5%, pH 7, filling volume 50 ml / 250 ml shake flask, sterilized at 115 degrees Celsius for 30 minutes, cooled after sterilization, Inoculate slant strains, cultivate at 30 degrees Celsius, 180 rpm for 10 hours, as liquid seeds;
[0082] Fermentation culture: Medium: beef extract 0.3%, peptone 1.0%, sodium chloride 0.5%, pH 7, filling volume 50ml / 250ml shake flask, sterilized at 115°C for 30 minutes, cooled and inoculated after sterilization Liquid seed, inoculum amount 10%, 30 degrees Celsius, 180 revolutions / min, cultivate for 10 hours.
[0083] 2. Biotransform...
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