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Method for rapidly measuring pentoses and hexose content in hemicellulose extract

A hemicellulose, rapid determination technology, applied in the direction of material analysis by observing the influence of chemical indicators, and analysis by chemical reaction of materials, etc., can solve the problem of low accuracy and reliability, poor repeatability, instrument High operation and maintenance costs, to achieve the effect of high accuracy and simple operation

Inactive Publication Date: 2009-07-29
SOUTH CHINA UNIV OF TECH
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  • Abstract
  • Description
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AI Technical Summary

Problems solved by technology

The first type is the traditional analytical method, the method for measuring total reducing sugar content such as 3,5-dinitrosalicylate colorimetric method, this method accuracy and reliability are not high; Dehydration under high temperature generates furfural or its derivatives, and then condenses with phenolic compounds to generate substances with special colors, such as orcinol-sulfuric acid method, phenol-sulfuric acid method and phloroglucinol-glacial acetic acid color development method, although the above methods operate Simple, but cannot realize the simultaneous determination of pentose and hexose, and the repeatability of the results of the first two methods is poor
The second category is modern instrumental analysis methods, mainly including gas chromatography, high performance liquid chromatography and ion chromatography. Although the accuracy of the analysis results is high, the equipment and sugar analysis columns used are expensive, and the cost of instrument operation and maintenance is high. Very high, and the pre-analysis sample processing procedures are complex

Method used

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  • Method for rapidly measuring pentoses and hexose content in hemicellulose extract
  • Method for rapidly measuring pentoses and hexose content in hemicellulose extract
  • Method for rapidly measuring pentoses and hexose content in hemicellulose extract

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Experimental program
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Effect test

Embodiment 1

[0022] Embodiment 1: the preparation of color reaction reagent

[0023] 1. Accurately weigh a certain amount of phloroglucinol (solid);

[0024] 2. Add a certain amount of glacial acetic acid, concentrated hydrochloric acid and absolute ethanol to the reagent bottle in turn, shake well to completely dissolve the phloroglucinol in step 1, and let it stand for later use. The ratio of phloroglucinol (g) to glacial acetic acid (mL), concentrated hydrochloric acid (mL) and absolute ethanol (mL) is 1:55:1:5.

Embodiment 2

[0025] Embodiment 2: monosaccharide color reaction

[0026] Pipette different concentrations of standard monosaccharides (glucose concentration range 0.5-5.0mmol / L, xylose concentration range 0.5-3.0mmol / L) and mixed sugar solution 1mL in a 25mL colorimetric tube, add 10mL of the mixture prepared in Example 1 Good color reaction reagents are mixed evenly and reacted in a boiling water bath for 10 minutes. Cool immediately after the reaction, and perform full-wavelength scanning in an ultraviolet-visible spectrophotometer, and use the blank sample to perform the same color reaction as a reference.

Embodiment 3

[0027] Embodiment 3: the mensuration of total sugar and pentose, hexose content

[0028] figure 1 It is the visible spectrum after color reaction of different concentrations of xylose and glucose. The figure shows that xylose and glucose with the same molar concentration have equal absorption at the same wavelength of 425nm after color reaction, so 425nm is the equimolar absorption wavelength of xylose and glucose. The relationship between the absorbance at the wavelength 425nm and the 553nm place and the total sugar and pentose concentration all follows Lambert-Beer's law, namely the standard curve y=0.1016x(R 2 = 0.9976) and y 1 =0.6628x(R 2 = 0.9986). Therefore, the total sugar, pentose and hexose content can be obtained by the following formula.

[0029] A 1 = ϵ 1 1 C 1 + ϵ 1 2 ...

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Abstract

The invention relates to a method for quickly determining contents of pentose and hexose in hemicellulose extracting solution. The method realizes simultaneous determination of the contents of total saccharide, the pentose and the hexose with a colorimetric method by a dual wavelength technology. The method helps greatly simplify a computing formula of the dual wavelength on the basis of equimolar absorption wavelength of the pentose and the hexose and characteristic absorption wavelength of the pentose, meanwhile, the method also helps verify that glucuronic acid and lignin probably present in the hemicellulose extracting solution have no significant interference on a determination result of the pentose and the hexose. The method has simple operation, quick speed and high accuracy, ensures higher recovery rate (from 97% to 103%) of determined total saccharide and various monosaccharide, can be fully realized by an ultraviolet-visible spectrophotometer, and is especially suitable for simultaneously determining and analyzing the contents of the total saccharide, the pentose and the hexose in the extracting solution of hemicellulose in broadleaf wood and grass materials (wherein, the content of pentosan is higher than that of hexosan).

Description

technical field [0001] The invention relates to a new method for rapidly measuring the content of sugar components in hemicellulose extract of plant raw materials. The dual-wavelength technology is used to realize the simultaneous determination of total sugar, pentose and hexose content, which is very suitable for polypentose content. Simultaneous analysis and determination of mixed sugar content in extracts of higher hemicelluloses (such as broad-leaved wood hemicellulose and grass raw material hemicellulose). Background technique [0002] At present, resource shortage and energy crisis are the focal issues faced by global development. Biomass resources, as a huge renewable resource, are getting more and more attention from the world. As a huge consumer of biomass resources, the pulp and paper industry is bound to face severe challenges. "Biomass refining" has become the direction and focus of domestic and foreign research in recent years. A group of foreign scholars put ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/78
Inventor 张曾迟聪聪
Owner SOUTH CHINA UNIV OF TECH
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