Method for extracting radiation resistant potentilla anserine polysaccharide oxide by ultrasonic waves

A technology of oxidizing fern hemp polysaccharide and ultrasonic wave is applied in the direction of antitoxic agent, pharmaceutical formula, medical preparation containing active ingredients, etc. chain degradation and other problems, to achieve significant comprehensive economic benefits, no toxic side effects, and good effects

Inactive Publication Date: 2010-01-06
陇西县正东中药材有限责任公司
View PDF10 Cites 11 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Patent ZL200610166326.7 authorizes an ultrasonic extraction method of rice bran polysaccharides. The ultrasonic power is 200-400W. The influence of extraction conditions on the purity of samples is not considered. The yield of purified polysaccharides is only 2.87%, which is difficult to apply. in actual production
[0006] Among the

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Method for extracting radiation resistant potentilla anserine polysaccharide oxide by ultrasonic waves
  • Method for extracting radiation resistant potentilla anserine polysaccharide oxide by ultrasonic waves
  • Method for extracting radiation resistant potentilla anserine polysaccharide oxide by ultrasonic waves

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] 1) Weigh a certain amount of dry prickly pear powder, place it in an ultrasonic extraction device, add 20 times (m / v) distilled water, stir and mix well; Filtrate under the condition of 0.07MPa, and the filtrate is concentrated under reduced pressure to 1 / 3 of the original volume under the condition of pressure 0.08MPa and temperature of 60°C, and ethanol is added to the concentrated solution (the ethanol content accounts for 75% of the total volume of the solution after adding ethanol) , after standing at room temperature for 30 hours, centrifuge at a speed of 4000 rpm, collect the lower sediment at -55°C, and freeze-dry at a pressure of 1 Pa for 28 hours to obtain substance A;

[0031] 2) Weigh a certain amount of substance A, dissolve it with 6 times the weight of distilled water, then add 10 μg / ml papain solution, the ratio of enzyme solution to A solution is 1:100 (v / v), enzymolyze 11 hour, then extracted 4 times with n-butanol and chloroform mixed solution (1:3, v...

Embodiment 2

[0034] 1) Weigh a certain amount of dry prickly pear powder, place it in an ultrasonic extraction device, add 35 times (m / v) distilled water, stir and mix well; Filtrate under the condition of 0.06MPa, and the filtrate is concentrated under reduced pressure to 1 / 9 of the original volume under the condition of pressure 0.07MPa and temperature of 55°C, and ethanol is added to the concentrated solution (the ethanol content accounts for 80% of the total volume of the solution after adding ethanol) After standing at room temperature for 36 hours, centrifuge at a speed of 5000 rpm, collect the lower precipitate and freeze-dry at -60°C and 13Pa for 30 hours to obtain substance A;

[0035] 2) Weigh a certain amount of substance A, dissolve it with 9 times the weight of distilled water, then add 10 μg / ml papain solution, the ratio of enzyme solution to A solution is 1:100 (v / v), enzymolyze 12 hours, then extracted 5 times with n-butanol and chloroform mixture (1:2, v / v), collected the ...

Embodiment 3

[0038] 1) Weigh a certain amount of dry prickly pear powder, place it in an ultrasonic extraction device, add 50 times (m / v) distilled water, stir and mix well; Filtrate under the condition of 0.08MPa, and the filtrate is concentrated under reduced pressure to 1 / 6 of the original volume under the condition of pressure 0.06MPa and temperature of 65°C, and ethanol is added to the concentrated solution (the ethanol content accounts for 70% of the total volume of the solution after adding ethanol) After standing still at room temperature for 24 hours, centrifuge at 4000 rpm, collect the lower sediment at 50°C, freeze-dry at 8 Pa for 24 hours to obtain substance A;

[0039] 2) Weigh a certain amount of substance A, dissolve it with 8 times the weight of distilled water, then add 10 μg / ml papain solution, the ratio of enzyme solution to A solution is 1:100 (v / v), and enzymolyze 10 hour, then extracted 3 times with n-butanol and chloroform mixture (1:4, v / v), collected the upper aque...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention provides a method for extracting radiation resistant potentilla anserine polysaccharide oxide by ultrasonic waves, which comprises the following steps: extracting potentilla anserine as raw material for 30 minutes-120 minutes by ultrasonic waves and filtering under the pressure condition of 0.06 MPa-0.08 Mpa; carrying out decompressing concentration, alcohol precipitation and centrifugation on filter liquor at the pressure of 0.06 Mpa-0.08 Mpa and the temperature of 55 DEG C-65 DEG C; collecting sediments at the lower layer; freezing and drying the sediments; after dissolving by nematolyt, extracting by mixture liquid of n-butyl alcohol and chloroform, decompressing, concentrating and dialyzing, obtaining natural potentilla anserine polysaccharide. The natural potentilla anserine polysaccharide has no toxic and side effect and has better repair effect on in-vivo oxidation damage caused by free radicals generated by X-ray radiation. An antioxidant ability experiment of a small mouse radiated by a 3.5Gy X-ray indicates that the SOD activity, the T-AOC vitality and the CAT vitality are respectively improved by 66.7 percent, 54.6 percent and 105.7 percent and the MDA content is reduced by 63.2 percent of an animal with radiation damage by taking the preparation for 7 days.

Description

technical field [0001] The invention relates to a preparation method of natural polysaccharides, in particular to a method for ultrasonically extracting fern polysaccharides with anti-radiation oxidation effect. Background technique [0002] The damage of ionizing radiation to organisms has both direct effects of energy transfer and indirect effects of a large number of free radicals generated through the radiation reaction of water. The base, ribose, and phosphate bonds in DNA molecules may all be attacked by free radicals, causing various types of damage such as base and ribose oxidation, chain breaks, and protein cross-linking. Lipid peroxidation induced by free radicals can cause an imbalance in the ratio of saturated and unsaturated fatty acids in biological membranes, change the rigidity and flexibility of membrane structures, increase membrane permeability, and even disintegrate. MDA is the decomposition product of lipid peroxidation, and its content can reflect the ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): C08B37/00A61K31/715A61P39/06A23L1/29A23L33/00
Inventor 张继赵保堂王俊龙吴依茜郭红云王小芳赵美荣姚健梁俊玉马君义杨云云
Owner 陇西县正东中药材有限责任公司
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap