Bacillus cereus and application thereof in promoting growth of poplars

A technology of Bacillus cereus and poplar, which is applied in the field of microbial fertilizer and biological fertilizer, can solve the problems of screening and application of organic phosphorus-resolving bacteria that have not been seen in poplar trees, and achieve excellent strain resources, good degradation effect, and promote growth and development. Effect

Active Publication Date: 2010-01-20
NANJING FORESTRY UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

At present, there are no reports on the screening and application

Method used

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  • Bacillus cereus and application thereof in promoting growth of poplars
  • Bacillus cereus and application thereof in promoting growth of poplars
  • Bacillus cereus and application thereof in promoting growth of poplars

Examples

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Example Embodiment

[0021] Example 1: Determination of the ability of strain JYZ-SD1 to solubilize phosphorus

[0022] Egg yolk medium: beef extract 3.0g, peptone 10.0g, sodium chloride 5.0g, distilled water 1000mL, agar 15.0~18.0g, pH 7.0, sterilized, when the temperature drops to about 50℃, add 3~4mL per 50mL Fresh egg yolk liquid (mix the egg yolk in equal volumes with sterile saline), mix well, and pour the plate.

[0023] The JYZ-SD1 strain that was activated twice was spot-attached to a yolk plate with a sterilized toothpick, and after 3 days of cultivation at 30°C, the diameter of the phosphate zone and the colony was measured, and the ratio of the phosphate zone to the colony diameter was calculated (Figure 2).

[0024] It can be seen from Figure 2 that after the JYZ-SD1 strain is cultured on the egg yolk plate, it can produce an obvious opaque phosphate ring. After measurement and calculation, the diameter of the phosphate ring is 25.81mm, the diameter of the colony is 4.28mm, and the ratio of...

Example Embodiment

[0025] Example 2: Determination of liquid phosphorus solubilization ability of strain JYZ-SD1

[0026] Monkinna solution organic phosphorus medium: glucose 10.0g, ammonium sulfate 0.5g, sodium chloride 0.3g, potassium chloride 0.3g, magnesium sulfate heptahydrate 0.3g, ferrous sulfate heptahydrate 0.03g, manganese sulfate tetrahydrate 0.03 g, lecithin 0.2g, calcium carbonate 5.0g, distilled water 1000mL, pH 7.0

[0027] Inoculate the activated JYZ-SD1 strain twice into NA medium (3.0g beef extract, 10.0g peptone, 5.0g sodium chloride, 1000mL distilled water, 15.0~18.0g agar, pH 7.2~7.4), and culture at 30℃ for 2 days , Rinse the bacteria with sterile water to make a bacterial suspension, take 1mL of the bacterial suspension in a 100mL Erlenmeyer flask containing 50mL of Monkina medium, and take the Monkina medium with 1mL of sterile bacterial suspension as the control (CK ), 3 repetitions for each treatment, 30℃, 180r·min -1 After shaking culture for 72h, the fermentation broth is...

Example Embodiment

[0029] Example 3: Greenhouse pot experiment of strain JYZ-SD1

[0030] After activating the strain JYZ-SD1 for 2 to 3 times, use an inoculation loop to pick a small amount of bacteria and inoculate a 100 mL triangle containing 50 mL of NB medium (3 g beef extract, 10 g peptone, 5 g sodium chloride, 1000 mL distilled water, pH 7.2 to 7.4) In the bottle, 30℃, 180r·min -1 Shake culture for 72h. Fermentation broth (4℃, 6000r·min -1 ) Centrifuge for 5 min, rinse the bacteria with sterile normal saline for 2 to 3 times, adjust the bacterial suspension with sterile normal saline (7~8×10 8 cfu / mL) made into bacteria. Inoculation of NL-895 cutting seedlings and seedlings of Populus nigra (seedling age 60 days), with the same amount of sterile saline as a control, the inoculation amount was 15 mL / plant of cutting seedlings and 5 mL / plant of seedlings. 6 replicates for each treatment, placed in the greenhouse for unified management, the light is 12h / day, and watered in time.

[0031] 150-da...

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Abstract

The invention discloses bacillus cereus JYZ-SD1 which is separated from the roots of poplars and can degrade lecithin. The classification designation of the bacillus cereus JYZ-SD1 is bacillus cereus. The bacillus cereus JYZ-SD1 is preserved in the China Center for Type Culture Collection (CCTCC), the preservation number is M 209096, and the preservation date is 4 May, 2009. The invention also discloses an application of the bacillus cereus in promoting the growth of the poplars. The JYZ-SD1 strain has stronger degradation capability to the lecithin; the JYZ-SD1 microbial agent is inoculated into NL-895 poplar cuttage seedlings and America black poplar tree seedlings; and a result shows that the microbial agent can obviously promote the growth of poplar seedlings. Therefore, the invention provides a superior strain resource for developing poplar phosphorus bacterial fertilizer.

Description

technical field [0001] The invention belongs to the technical field of microbial fertilizers in the field of biological fertilizers, and in particular relates to a bacillus cereus capable of efficiently dissolving phosphorus in the rhizosphere of poplars and its application in promoting the growth of poplars. Background technique [0002] Phosphorus plays an important role in the process of plant growth and development, and is one of the important nutrients necessary for plants. One-third to one-half of the cultivated soil in my country is deficient in phosphorus, but this kind of phosphorus deficiency is "genetic phosphorus deficiency" rather than "soil science phosphorus deficiency", that is, the soil available phosphorus content is very low, and most of the soil Phosphorus is in the form of organic and inorganic phosphides that cannot be absorbed and utilized by plants. Among them, organophosphorus compounds are an important part of soil phosphorus, accounting for about 1...

Claims

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Application Information

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IPC IPC(8): C12N1/20C05F11/08C12R1/085
Inventor 吴小芹刘辉叶建仁王进杰
Owner NANJING FORESTRY UNIV
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