Bacillus cereus and application thereof in promoting growth of poplars

A technology of Bacillus cereus and poplar, which is applied in the field of microbial fertilizer and biological fertilizer, can solve the problems of screening and application of organic phosphorus-resolving bacteria that have not been seen in poplar trees, and achieve excellent strain resources, good degradation effect, and promote growth and development. Effect

Active Publication Date: 2010-01-20
NANJING FORESTRY UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there are no reports on the screening and application of poplar organophosphate-decomposing bacteria at home and abroad

Method used

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  • Bacillus cereus and application thereof in promoting growth of poplars
  • Bacillus cereus and application thereof in promoting growth of poplars
  • Bacillus cereus and application thereof in promoting growth of poplars

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] Example 1: Determination of Phosphorus Solubilizing Ability of Bacterial Strain JYZ-SD1 Plate

[0022] Egg yolk medium: 3.0g beef extract, 10.0g peptone, 5.0g sodium chloride, 1000mL distilled water, 15.0-18.0g agar, pH 7.0, sterilized, when the temperature drops to about 50℃, add 3-4mL to every 50mL Fresh egg yolk liquid (egg yolk mixed with sterile saline in equal volumes), mix well, pour plate.

[0023] The JYZ-SD1 strain activated twice was spotted on the egg yolk plate with a sterilized toothpick, and after culturing at 30°C for 3 days, the diameter of the phosphorus-dissolving circle and the colony were measured, and the ratio of the phosphorus-dissolving circle to the diameter of the colony was calculated (Figure 2).

[0024] It can be seen from Figure 2 that after the JYZ-SD1 strain was cultured on the egg yolk plate, it could produce an obviously opaque phosphorus-dissolving circle. After measurement and calculation, the diameter of the phosphorus-dissolving c...

Embodiment 2

[0025] Embodiment 2: Determination of the liquid phosphorus solubilizing ability of bacterial strain JYZ-SD1

[0026] Montina solution organic phosphorus medium: glucose 10.0g, ammonium sulfate 0.5g, sodium chloride 0.3g, potassium chloride 0.3g, magnesium sulfate heptahydrate 0.3g, ferrous sulfate heptahydrate 0.03g, manganese sulfate tetrahydrate 0.03 g, lecithin 0.2g, calcium carbonate 5.0g, distilled water 1000mL, pH 7.0

[0027] Inoculate the twice activated JYZ-SD1 strain into NA medium (3.0g of beef extract, 10.0g of peptone, 5.0g of sodium chloride, 1000mL of distilled water, 15.0-18.0g of agar, pH 7.2-7.4), and culture at 30°C for 2 days , rinse the bacteria with sterile water to make a bacterial suspension, take 1mL of the bacterial suspension and put it in a 100mL Erlenmeyer flask containing 50mL of Montina medium, and take Montina medium with 1mL of sterile suspension as a control (CK ), 3 repetitions for each treatment, 30°C, 180r·min -1 After 72 hours of shakin...

Embodiment 3

[0029] Embodiment 3: the greenhouse pot experiment of bacterial strain JYZ-SD1

[0030]After activating the strain JYZ-SD1 for 2 to 3 times, use an inoculation loop to pick a small amount of bacteria and inoculate it into 100 mL of triangular medium containing 50 mL of NB medium (3g of beef extract, 10g of peptone, 5g of sodium chloride, 1000mL of distilled water, pH 7.2 to 7.4). In a bottle, 30°C, 180r·min -1 Shake culture for 72h. Fermentation broth (4°C, 6000r min -1 ) centrifuged for 5min, rinsed the bacteria with sterile normal saline for 2 to 3 times, and adjusted the bacterial suspension with sterile normal saline (7~8×10 8 cfu / mL) to make bacterial agent. NL-895 cutting seedlings and black poplar seedlings (60 days old) were inoculated respectively, and the same amount of sterile saline was used as the control, and the inoculation volume was 15 mL / plant for cutting seedlings and 5 mL / plant for seedlings. 6 replicates per treatment were placed in the greenhouse for ...

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Abstract

The invention discloses bacillus cereus JYZ-SD1 which is separated from the roots of poplars and can degrade lecithin. The classification designation of the bacillus cereus JYZ-SD1 is bacillus cereus. The bacillus cereus JYZ-SD1 is preserved in the China Center for Type Culture Collection (CCTCC), the preservation number is M 209096, and the preservation date is 4 May, 2009. The invention also discloses an application of the bacillus cereus in promoting the growth of the poplars. The JYZ-SD1 strain has stronger degradation capability to the lecithin; the JYZ-SD1 microbial agent is inoculated into NL-895 poplar cuttage seedlings and America black poplar tree seedlings; and a result shows that the microbial agent can obviously promote the growth of poplar seedlings. Therefore, the invention provides a superior strain resource for developing poplar phosphorus bacterial fertilizer.

Description

technical field [0001] The invention belongs to the technical field of microbial fertilizers in the field of biological fertilizers, and in particular relates to a bacillus cereus capable of efficiently dissolving phosphorus in the rhizosphere of poplars and its application in promoting the growth of poplars. Background technique [0002] Phosphorus plays an important role in the process of plant growth and development, and is one of the important nutrients necessary for plants. One-third to one-half of the cultivated soil in my country is deficient in phosphorus, but this kind of phosphorus deficiency is "genetic phosphorus deficiency" rather than "soil science phosphorus deficiency", that is, the soil available phosphorus content is very low, and most of the soil Phosphorus is in the form of organic and inorganic phosphides that cannot be absorbed and utilized by plants. Among them, organophosphorus compounds are an important part of soil phosphorus, accounting for about 1...

Claims

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Application Information

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IPC IPC(8): C12N1/20C05F11/08C12R1/085
Inventor 吴小芹刘辉叶建仁王进杰
Owner NANJING FORESTRY UNIV
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