New strain of bifidobacterium and fermentative preparation method and application thereof

A bifidobacterium, fermentation and cultivation technology, applied in the field of microorganisms, can solve the problems of less reports on the impact of the application effect of stress-resistant product preparations on the quality of animal products, late bifidobacterium preparations, etc., and achieve strong tolerance to adverse environments , good antibacterial activity, fast growth effect

Active Publication Date: 2010-03-17
CHINA AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Domestic research on bifidobacterium preparations started relatively late, and there are few applications on animals. Only some experimental research reports have been seen, especially in the field of animal s...

Method used

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  • New strain of bifidobacterium and fermentative preparation method and application thereof
  • New strain of bifidobacterium and fermentative preparation method and application thereof
  • New strain of bifidobacterium and fermentative preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0074] Embodiment 1 Fermentation method A1

[0075] Use 0.7% glucose, 0.6% soybean peptone, 0.4% tryptone, 0.3% soybean peptide, 1.2% corn dregs, 0.5% ammonium citrate (the above are mass volume ratio m / V), 0.4% mixed salt solution (V / V), defoamer M 0.05% (V / V) (Beijing Chemical Industry Group, model 2118) was prepared in proportion as a fermentation culture medium. After sterilizing by high-temperature steam at 121°C for 20 minutes, when the temperature was lowered to 37°C, inoculate 1.5% of the 15-hour seed liquid into it, and at 35°C, stir at 100 rpm every 2-3 hours for 10 minutes. 15 hours of inoculation is the end point of fermentation, and it can be placed in a tank, and the number of viable bacteria of Bifidobacterium breve BB2 is 4.76×10 8 CFU / mL.

[0076] After centrifugation, the bacteria slime was collected and added with a freeze-drying protective agent (1.8% stachyose, 0.3% sodium glutamate, 0.6% galactose, 12% skim milk powder), and then freeze-dried t...

Embodiment 2

[0078] Embodiment 2 fermentation method A2

[0079] With 1.2% of glucose, 0.3% of soybean peptone, 0.7% of tryptone, 0.5% of soybean peptide, 1.0% of corn dregs, 0.6% of ammonium citrate (the above are mass volume ratio m / V), add the mixed salt used in Example 1 0.5% (V / V) of the solution and 0.05% (V / V) of the antifoaming agent M are prepared in proportion as a fermentation culture medium. After sterilizing by high-temperature steam at 121°C for 20 minutes, when the temperature was lowered to 37°C, inoculate 2.5% of the 25-hour seed liquid into it, and at 35°C, stir at 100 rpm every 2-3 hours for 10 minutes. 15 hours of inoculation is the end point of fermentation, and it can be placed in a tank, and the number of viable bacteria of Bifidobacterium breve BB2 is 5.19×10 8 CFU / mL.

[0080] After centrifugation, the bacteria slime was collected and added with a freeze-drying protective agent (1.8% stachyose, 0.3% sodium glutamate, 0.6% galactose, 12% skim milk powder),...

Embodiment 3

[0081] Embodiment 3 fermentation method A3

[0082] With 1.5% of glucose, 0.5% of soybean peptone, 0.7% of tryptone, 0.4% of soybean peptide, 0.6% of corn dregs, 0.8% of ammonium citrate (the above are mass volume ratio m / V), add the mixed salt used in Example 1 0.3% (V / V) of the solution and 0.05% (V / V) of the antifoaming agent M are prepared in proportion as a fermentation culture medium. After sterilizing by high-temperature steam at 121°C for 20 minutes, when the temperature was lowered to 37°C, inoculate 2.0% of the 12-hour-old seed liquid into it, and at 35°C, stir at 120 rpm every 2-3 hours for 10 minutes. 14 hours of inoculation is the end point of fermentation, and it can be placed in a tank, and the number of viable bacteria of Bifidobacterium breve BB2 is 6.47×10 8 CFU / mL.

[0083] After centrifugation, the bacteria slime was collected and added with a freeze-drying protective agent (1.8% stachyose, 0.3% sodium glutamate, 0.6% galactose, 12% skim milk powd...

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Abstract

The invention relates to a bifidobacterium BB2 that is separated from the alimentary canal of healthy broilers and can generate bacteriocin or bacteriostatic substances, and the collection number is CGMCC No. 3200. The strain has the advantages of strong stress resistance, rapid growth, high acidness of fermentation liquor, large biomass, bacteriostatic or antibacterial activity and the like. Theinvention also relates to a large-scale fermentative culture method of the strain, and bactericide with viable count of 12-15 billion/g can be obtained by centrifuging the fermentation liquor to recover bacterial sludge and adding protective agent after freeze drying. The bifidobacterium subspecies BB2 preparation can partially substitute for antibiotics, eliminate drug residues, improve the quality of animal products, animal productivity and immunity or disease resistance, reduce the mortality and elimination rate of animals and diarrhoea rate, and also purify the environment of animal houses. The strain, the method and the application have high social, economic and ecological benefits.

Description

technical field [0001] The invention relates to the technical field of microorganisms, in particular to a new strain of Bifidobacterium subspecies capable of producing bacteriocins or antibacterial substances, and also relates to a fermentation preparation method and application of the strain. Background technique [0002] In recent years, due to the needs of food safety, the research and development of microecological preparations has developed rapidly. However, most of the strains currently used are Lactobacillus, Saccharomyces, and Bacillus, but Bifidobacteria are rarely used. The reason is that bifidobacteria usually have very high nutritional requirements, and the growth conditions must be strictly anaerobic. The high cost of cultivation and strict production conditions make most bifidobacteria unable to meet the requirements of research and development and even actual production. [0003] However, bifidobacteria are still favored in the research of microecological prep...

Claims

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Application Information

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IPC IPC(8): C12N1/20A23K1/16A61K35/74A61P3/06A61P31/04A61P35/00A61P37/04C12R1/01A23K10/18A61K35/745
Inventor 张日俊梁晓明黄燕李桂霞
Owner CHINA AGRI UNIV
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