Method for producing high protein bean pulp based on two-step microbial fermentation
A two-step fermentation, high-protein technology, applied in the direction of animal feed, animal feed, application, etc., can solve the problems of neglected protein, low digestibility and biological titer, affecting the absorption and utilization of soybean protein, etc., to improve protein quality, The effect of increasing animal feed intake and improving palatability
Inactive Publication Date: 2012-07-18
DALIAN POLYTECHNIC UNIVERSITY
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Problems solved by technology
However, previous reports often only focused on the protein content and ignored the protein quality. 80% of the protein in soybeans has a molecular weight of more than 100kd, and there are problems of low digestibility and biological potency, which largely affects the absorption of soybean protein. use
Method used
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Experimental program
Comparison scheme
Effect test
Embodiment
[0020] 1. Raw materials and processing
[0021] Soybean meal, corn and bran were crushed to 80 mesh, mixed evenly according to the ratio of soybean meal: bran: corn=7:2:1, and steam sterilized at 100°C for 25 minutes.
[0022] 2. Activation of fermentation strains
[0023] Bacillus subtilis was activated and cultivated in a slant culture mode, and the medium was beef extract peptone medium, and the activation time was 20 hours.
[0024] Saccharomyces cerevisiae was activated and cultivated in a slant culture mode, the medium was wort juice, and the activation time was 20 hours.
[0025] Three, two-step fermentation method
[0026] First insert Bacillus subtilis into the material with an inoculation amount of 1-5%, add 0.4-1.2 times the sterile water with urea and ammonium sulfate according to the mass ratio, and ferment at 30-50°C for 12-48 hours; then insert 1-10% Saccharomyces cerevisiae, continue to ferment for 24-48 hours at 20-40°C.
[0027] 4. Material drying
[002...
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The invention discloses a method for producing high protein bean pulp based on two-step microbial fermentation, which takes bean pulp as a main raw material as well as corn and wheat bran as auxiliary materials; the materials are ground, evenly mixed together and firstly inoculated with bacillus subtilis for solid state fermentation and then saccharomyces cerevisiae for fermentation to obtain thefinal product; and after being dried at the temperature of 40-80 DEG C, the product can be taken as animal feed. By adopting multi-strain solid state fermentation and utilizing mixed bacteria synergy, the method not only increases the protein content of the bean pulp, but also degrades macromolecule protein which is difficult to digest in the bean pulp to a certain degree. Simultaneously, the palatability of the fermented bean pulp can be improved by mellow smell generated in the process of fermentation of yeast, so that feed intake can be increased.
Description
technical field [0001] The invention relates to a processing method of animal feed, in particular to a method for producing soybean meal feed based on microbial fermentation technology. Background technique [0002] Soybean is an excellent plant protein resource. The processed soybean meal has the characteristics of high digestion and absorption rate, good amino acid composition, reasonable price and abundant resources. It is one of the available plant protein sources in the feed industry. Soybean meal is a by-product of soybean oil extracted by leaching. It is cheap and has a wide range of sources. Its protein content is as high as 43%-48%. Except for the lack of sulfur-containing amino acids, the rest of the amino acid composition is reasonable, especially for other plant feeds Lysine, which is easily lacking, has a content as high as 2%-2.5%. Soybean meal also contains biologically active substances such as isoflavones and phospholipids, as well as other nutrients such as...
Claims
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IPC IPC(8): A23K1/14A23K10/12A23K10/30A23K10/37
CPCY02P60/87
Inventor 王际辉刘稳结
Owner DALIAN POLYTECHNIC UNIVERSITY