Copolymer of fibroin and poly L-lactic acid and preparation method and application thereof

A silk protein and copolymer technology, applied in the field of silk protein and poly-L-lactic acid copolymer and its preparation, can solve the problem of no research report

Inactive Publication Date: 2010-06-02
NANJING FORESTRY UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The molecular weight of poly L-lactic acid prepolymer can be precisely controlled, and the molecular weight of the copolymer can be increased by increasing the molecular weight of poly L-lactic acid prepolymer and the molecular weight of silk fibroin respectively, and different L-lactide chains can be obtained. segment length diblock polymers, but there is no research report in this area at home and abroad

Method used

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  • Copolymer of fibroin and poly L-lactic acid and preparation method and application thereof
  • Copolymer of fibroin and poly L-lactic acid and preparation method and application thereof
  • Copolymer of fibroin and poly L-lactic acid and preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0063] L-lactide, SnCl 2 / p-toluenesulfonic acid catalyst system (SnCl 2 The molar content accounts for 50% of the entire catalyst system, SnCl 2 The amount used is 0.5wt% of L-lactide. ) into the polymerization kettle. The pressure of the system was gradually reduced to 0 mmHg (manometer reading) and then sealed. The reaction temperature was 170° C., and the reaction was carried out for 10 hours to obtain a poly-L-lactic acid prepolymer (molecular weight: 12,000). Then dehydrated silk protein powder (the number-average molecular mass is 3000, the mass ratio of L-lactide to silk protein is 4:1) is added into the reactor, and the reaction condition is maintained for 10 hours. Then the temperature was lowered to 140° C., and a solid phase reaction was carried out for 2 hours. After the polymerization, the purified copolymer of silk protein and poly-L-lactic acid is a yellow powder with a molecular weight of 16,000, a yield of 73%, and a specific rotation of 93°.

Embodiment 2

[0065] L-lactide, SnCl 2 / naphthalenesulfonic acid catalyst system (SnCl 2 The dosage is 0.5wt% of L-lactide, SnCl 2 The molar content accounts for 50% of the whole catalyst system) into the polymerization tank. The pressure of the system was gradually reduced to 10 mm Hg and then sealed. The reaction temperature was 170° C. and the reaction time was 5 hours to obtain a poly-L-lactic acid prepolymer (molecular weight: 8,000). Then dehydrated silk protein powder (the number-average molecular mass is 3000, the mass ratio of L-lactide to silk protein is 6:1) was added into the reactor, and the reaction conditions were maintained for 7 hours. Then the temperature was lowered to 130° C., and the solid state reaction was carried out for 10 hours. After the polymerization, the purified copolymer was a yellow powder with a molecular weight of 24,000, a yield of 73%, a melting point of 126°C and a specific rotation of 113°.

Embodiment 3

[0067] L-lactide, stannous isooctanoate / naphthalenesulfonic acid catalyst system (the consumption of stannous isooctanoate is 0.5wt% of L-lactide, and the molar content of stannous isooctanoate accounts for 50% of the whole catalyst system) Add to the polymerization kettle. The pressure of the system was gradually reduced to 2 mm Hg and then sealed, the reaction temperature was 170° C., and the reaction time was 2 hours to obtain a poly-L-lactic acid prepolymer (molecular weight: 5,000). Then dehydrated silk protein powder (the number-average molecular mass is 1000, and the mass ratio of L-lactide to silk protein is 10:1.) was added to the reaction kettle, and the reaction conditions were maintained to continue the reaction for 7 hours. Then the temperature was lowered to 140° C., and the solid phase reaction ended after 10 hours. The purified copolymer is a yellow powder with a molecular weight of 29,000, a yield of 74%, a melting point of 130°C and a specific rotation of 11...

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Abstract

The invention discloses a copolymer of fibroin and poly L-lactic acid, which comprises the following structure. The invention also discloses a preparation method for the copolymer through melt polymerization and application of the copolymer in biomedical materials. The preparation method comprises the following steps of: preparing a prepolymer of the poly L-lactic acid by performing ring-opening polymerization on L-lactide in a molten state; and performing melt / solid phase copolymerization with the fibroin to prepare the copolymer of the fibroin and the poly L-lactic acid. The molecular weight of the prepolymer of the poly L-lactic acid can be controlled precisely to obtain a block polymer with different L-lactide chain segment lengths from short chains to long chains. A solvent is not used in the polymerization process, so the process is simple and the product cost is low. The preparation method can prepare the copolymers of the fibroin and poly the L-lactic acid with different number-average molecular weights. The copolymer of the fibroin and poly the L-lactic acid prepared by the method can improve the hydrophilic property, the biocompatibility and the cellular affinity of a polylactic acid material, and control the degrading speed of the material.

Description

Technical field: [0001] The invention belongs to the technical field of polymer materials, and in particular relates to a copolymer of silk protein and poly-L-lactic acid, a preparation method and application thereof. Background technique: [0002] Polylactic acid (PLA) is a biodegradable and absorbable polymer material with good biocompatibility. It has excellent mechanical properties, and its raw material can be prepared by fermentation of starch to realize the regeneration of resources. PLA has been widely used in tissue engineering and other biomedical fields, and its degradation products can participate in the metabolism of the human body with low toxicity. PLA is a hydrophobic polymer with poor adhesion to cells. Pure polylactic acid as a tissue engineering material will cause some mild inflammatory reactions after being implanted into the organism. The brittleness of polylactic acid is high, the mechanical strength is low, and the degradation cycle is difficult to c...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08G81/00C08H1/00C08G63/685C08G63/08A61L27/22A61L27/58A61L33/12A61K47/42
Inventor 高勤卫李明子查震源赵小玉
Owner NANJING FORESTRY UNIV
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