Bio-hydrogen production method by photosynthetic bacteria

A technology for photosynthetic bacteria and hydrogen production, which is applied in the fields of biotechnology and microbiology, and can solve problems such as light bulbs easily polluting the environment, threatening the normal growth of photosynthetic bacteria, and high temperature in the fermentation system

Inactive Publication Date: 2010-06-30
SHANGHAI INST OF BIOLOGICAL SCI CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although traditional incandescent lamps can meet the needs of photosynthetic bacteria to produce hydrogen by anaerobic fermentation, most of their electric energy is lost in the form of heat, and the light energy produced only accounts for a small part, and the energy utilization efficiency is very low, resulting in a large energy consumption. waste, and the heat emitted by incandescent lamps will cause excessive temperature in the entire fermentation system itself, affecting the normal hydrogen production of photosynthetic bacteria, and even threatening the normal growth of photosynthetic bacteria
At the same time, incandescent lamps also have the disadvantages of short life, waste bulbs are easy to pollute the environment, etc.

Method used

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  • Bio-hydrogen production method by photosynthetic bacteria

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0060] Example 1: Biological hydrogen production by photosynthetic bacteria under 1W high-power LED single-side irradiation

[0061] 1. Photosynthetic bacterial strains

[0062]The photosynthetic bacteria strain that adopts is Rhodobacter sphaeroides ZX-5 (Yongzhen Tao, et al.Characteristics of a new photosynthetic bacterial strain for hydrogen production and its application in wastewater treatment. International Journal of Hydrogen Energy, 2008, 33: 963-973).

[0063] 2. Photosynthetic Bacteria Fermentation Hydrogen Production Medium Formula (1L)

[0064] (1) 50mL of basic salt solution;

[0065] (wherein, 1L basic salt solution contains MgSO 4 ·7H 2 O 4g, CaCl 2 ·H 2 O 1.5g, FeSO 4 ·7H 2 O0.236g, Na 2 EDTA 0.4g) 50mL; trace element solution 20mL (wherein 100mL trace element solution contains MnSO 4 ·7H 2 O 0.21g, H 3 BO 3 0.28g, Cu(NO 3 ) 2 ·7H 2 O 0.004g, ZnSO 4 ·7H 2 O 0.024g, Na 2 MoO 4 2H 2 O 0.075g));

[0066] (2) 1 mL of vitamin solution (100 mL ...

Embodiment 2

[0080] Example 2: Biological hydrogen production by photosynthetic bacteria under 1W high-power LED double-sided irradiation of monochromatic light

[0081] 1. Photosynthetic bacterial strains

[0082] The photosynthetic bacteria strain was Rhodopseudomonas palustris 1.2181 (purchased from China General Microorganism Culture Collection and Management Center).

[0083] 2. Photosynthetic Bacteria Fermentation Hydrogen Production Medium Formula (1L)

[0084] (1) 50mL of basic salt solution;

[0085] (wherein, 1L basic salt solution contains MgSO 4 ·7H 2 O 4g, CaCl 2 ·H 2 O 1.5g, FeSO 4 ·7H 2 O0.236g, Na 2 EDTA 0.4g, trace element solution 20mL (100mL trace element solution contains MnSO 4 ·7H 2 O 0.21g, H 3 BO 3 0.28g, Cu(NO 3 ) 2 ·7H 2 O 0.004g, ZnSO 4 ·7H 2 O 0.024g, Na 2 MoO 4 2H 2 O 0.075g));

[0086] (2) Vitamin solution 1mL (100mL vitamin solution contains Nicotinic Acid 1g, Thiamine·HCl 0.5g, Biotin 0.01g);

[0087] (3) 4g of succinic acid;

[0088]...

Embodiment 3

[0097] Example 3: Biological hydrogen production by photosynthetic bacteria under 1W high-power LED combined double-sided irradiation

[0098] 1. Photosynthetic bacterial strains

[0099] The photosynthetic bacterial strain used was Rhodobacter sphaeroides ZX-5.

[0100] 2. Photosynthetic Bacteria Fermentation Hydrogen Production Medium Formula (1L)

[0101] (1) Basic salt solution 50mL

[0102] (wherein, 1L basic salt solution contains MgSO 4 ·7H 2 O 4g, CaCl 2 ·H 2 O 1.5g, FeSO 4 ·7H 2 O0.236g, Na 2 EDTA 0.4g, trace element solution 20mL (wherein 100mL trace element solution contains MnSO 4 ·7H 2 O 0.21g, H 3 BO 3 0.28g, Cu(NO 3 ) 2 ·7H 2 O 0.004g, ZnSO 4 ·7H 2 O 0.024g, Na 2 MoO 4 2H 2 O 0.075g));

[0103] (2) Vitamin solution 1mL (100mL vitamin solution contains Nicotinic Acid 1g, Thiamine·HCl 0.5g, Biotin 0.01g);

[0104] (3) Malic acid 4g;

[0105] (4) Phosphate buffer (0.2M, pH7.0) 7.5ml;

[0106] (5) Glutamic acid 1g.

[0107] 3. Conditions for ...

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Abstract

The invention relates to a bio-hydrogen production method by photosynthetic bacteria, which comprises the following step: (1) under an anaerobic condition, an organic acid is used as a fermentation substrate, the photosynthetic bacteria are fermented and cultured under one or a plurality of light sources with the wavelengths selected from the ranges of 400-500nm, 510-550nm, 575-600nm, 600-640nm and 780-880nm or the white light corresponding color temperature range of 2650-10000K, and the light intensity is 3000-7000Lux, so mixed gas is generated; and (2) hydrogen is separated from the generated mixed gas. With a proper light source, the invention can provide adequate light energy for hydrogen production by fermenting the photosynthetic bacteria, greatly increases the efficiency of energy conversion, and avoids the energy consumption steps of temperature reduction and the like on the whole fermentation system.

Description

technical field [0001] The invention belongs to the fields of biotechnology and microbiology, and more specifically, the invention relates to an optimized, energy-saving and high-efficiency photosynthetic bacteria biological hydrogen production method. Background technique [0002] With the rapid development of social economy and the continuous improvement of people's living standards, the demand for energy in the whole society is getting higher and higher. However, the current three major fossil energy sources, natural gas, coal and oil, are about to be exhausted, and the two major problems of energy crisis and environmental pollution have become the focus of the world. [0003] Due to its low density and high combustion calorific value, hydrogen only produces water vapor when converted into heat or electricity, and does not produce toxic gases and greenhouse gases. It is the cleanest and environmentally friendly energy source, especially suitable for transportation, and is...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P3/00C12R1/01
Inventor 周志华肖伟宗文明孙琴史吉平
Owner SHANGHAI INST OF BIOLOGICAL SCI CHINESE ACAD OF SCI
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