Biomimetic porous microsphere tissue engineering scaffold and manufacturing method thereof

Abstract

The invention discloses a biomimetic porous microsphere tissue engineering scaffold and a manufacturing method thereof. The scaffold is a microsphere manufactured by imitating natural extracellular matrix; the diameter ranges from 100 micrometers to 500 micrometers; cell holes and material exchange holes are distributed on the surface of the scaffold; three-dimensional network structures which are communicated with each other are arranged in the scaffold. When in use, a certain quantity of the scaffolds form various scaffolds which can be seen by the naked eyes by using a tailor made die or a tissue engineering reactor. The scaffold comprises natural biological materials of collagen and chitosan, and the manufacturing method of the scaffold comprises the following steps of: (1) mixing 8 to 10 percent of collagen acetic acid dispersion liquid and chitosan acetic acid dispersion liquid in certain proportion; (2) mixing mixed liquid obtained in the step (1) and liquid paraffin containing 6 to 8 percent of Span-85; (3) adding mixed liquid obtained in the step (2) to the liquid paraffin in volume ratio of 1 to 5, severely stirring and crosslinking with glutaraldehyde to obtain a crude product of the tissue engineering scaffold; and (4) respectively washing the crude product obtained in the step (3) with distilled water, isopropyl alcohol, ether and absolute ethyl alcohol and screening to obtain a finished product of the tissue engineering scaffold. The invention can be used as a tissue engineering scaffold, inhibit the tumor-induced trend in the process of stem cell growing development and promote the regeneration of blood vessels and nerve, and the rate of degradation and absorption is adapted to the formation of tissue organs. In addition, the invention can also be used for injectable tissue engineering scaffolds, great proliferation of seed cells used for tissue engineering or vectors for stem cell transfer for treatment.

Description

technical field [0001] The invention belongs to the field of biomedical materials, and in particular relates to a bionic porous microsphere tissue engineering scaffold, which can be used clinically for: ① The bionic tissue engineering scaffold is used for three-dimensional cultivation of stem cells to regenerate various tissues and organs for clinical transplantation; ②Massive expansion of seed cells for tissue engineering; ③Injectable tissue engineering scaffolds or carriers for stem cell transfer for treatment. Background technique [0002] The use of stem cell tissue engineering technology to regenerate human tissues and organs (such as skin, blood vessels, liver, etc.) is an important way to solve the current clinical problem of "repairing damage from damage" and the severe shortage of organs available for clinical transplantation. Tissue engineering scaffold (tissue engineering scaffold) is a substitute for extracellular matrix, which is the basis of tissue engineering....

AI Technical Summary

Problems solved by technology

Existing porous scaffolds still have the following defects: ① irregular shape, mainly flakes (Xinetal. Lumpy ( et al.Evaluation of RGD-or EGF-immobilized chitosan scaffolds for chondrogenic activity.International Journal of Biological Macromolecules, (43):121-128, 2008), solid microspheres (Blaker et al.Novel fabrication techniques to producemicrospheres by thermally induced par for tissue engineering and drug delivery. ActaBiomaterialia, (4): 264-272, 2008), disc-shaped (Wang et al. In vitrocartilage tissue engineering with 3D porous aqueous-derived silkscaffolds and mesenchymal stem cells. Biomaterials, (26): 7082 -7094, 2005), tubular (Nieponice et al. Development of a tissue-engineered vascular graft combining abiodegradable scaffold, muscle-derived stem cells and arotational vacuum seeding technique. Biomaterials, (29): 825-833, 2008), fibrous (Danielsson et al. Modified collagenfleece, a scaffold for transplantation of human bladder smoothmuscle cells. Biomaterials, (27): 1054-1060, 2006) and other shapes, the specific surface area of ​​these shapes is small, which is not conducive to cell growth and differentiation, in practical applications ②Due to the manufacturing method, the geometric structure of the inner hole of the scaffold is not conducive to three-dimensional cell culture and tissue regeneration; ③Due to the irregular shape of the scaffold and the unreasonable internal geometric structure, the regenerated tissue lacks the development of blood vessels and nerves. natural space

Images