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Composite functional liposome and preparation method and application thereof

A compound function and liposome technology, which is applied in the direction of liposome delivery, pharmaceutical formulations, medical preparations of non-active ingredients, etc., can solve the problems of irritation and perishability, achieve stable properties, improve stability, Effect of Good Encapsulation Efficiency

Inactive Publication Date: 2010-09-01
GUANGDONG PHARMA UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The object of the present invention is to provide a composite functional liposome according to the problems of existing cosmetic additives such as easy deterioration or irritation, which can embed a variety of cosmetic additives, delay the oxidative deterioration of drugs, and utilize the liposome affinity and promote skin penetration to increase the effective absorption of drugs and reduce the irritation of drugs

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] Dissolve vitamin C in a small amount of Na 2 HPO 4 -K 2 HPO 4 buffer solution, Na was added dropwise 2 HPO 4 Adjust the pH of the aqueous solution to 5, and prepare the concentration of 0.04 g / mL with a buffer solution. Dissolve 0.15g of soybean lecithin, 0.04g of cholesterol, and 0.03g of salicylic acid in 4mL of ether, add 1mL of the above-mentioned vitamin C aqueous solution, the power is 90W, and the temperature of the water bath is 30°C. for colostrum. Add colostrum to 10 mL of Na at pH = 7.4 2HPO 4 -K 2 HPO 4 In the buffer solution, the power is 100W, the temperature of the water bath is 45°C, ultrasonic crushing is performed for 6 minutes, until the oil layer completely disappears, and a uniform and slightly transparent emulsion is formed, which is W / O / W double emulsion. Thereafter, rotary steaming was performed under reduced pressure at 35°C for 8 min until there was no ether smell. The prepared liposome emulsion is packed into a centrifuge tube with ...

Embodiment 2

[0029] Dissolve 0.15g of soybean lecithin, 0.04g of cholesterol, and 0.03g of salicylic acid in 4mL of ether, add 1mL of vitamin C aqueous solution with a pH of 3.8 and a concentration of 0.04g / mL, with a power of 90W and a water bath temperature of 25°C for 2min to A homogeneous turbid liquid formed by the disappearance of the layer. Add 10 mL of Na with pH=7.4 to the prepared colostrum 2 HPO 4 -K 2 HPO 4 In the buffer solution, the power is 100W, and the temperature of the water bath is 40°C, sonicate for 7 minutes until the oil layer disappears to form a uniform and slightly transparent double emulsion. Thereafter, rotary steaming was performed under reduced pressure at 30°C for 10 min until there was no ether smell. The prepared liposome emulsion is packed into a centrifuge tube with a volume of 2mL, centrifuged for 1h, and the rotating speed is 10000rad / min, and the supernatant is taken for ultraviolet absorption measurement, and the encapsulation efficiency of vitami...

Embodiment 3

[0031] Take 1.5mL vitamin C aqueous solution with a pH of 5 and a concentration of 0.04g / mL, add 4mL of ether solution dissolved with 0.2g soybean lecithin, 0.04g cholesterol, and 0.04g salicylic acid, with a power of 90W and a water bath temperature of 30°C Ultrasound for 2 minutes until the stratification disappears and uniform turbid colostrum is formed. Add colostrum to 10 mL of Na at pH = 7.4 2 HPO 4 -K 2 HPO 4 In the buffer solution, the power is 100W, the temperature of the water bath is 45°C, and ultrasonic crushing is carried out for 6 minutes until the oil layer disappears completely, and a uniform and slightly transparent double emulsion is formed. Then, it was rotary evaporated under reduced pressure at 35°C for 10 min until there was no ether smell. The prepared liposome emulsion is packed into a centrifuge tube with a volume of 2mL, centrifuged for 1h, and the rotating speed is 10000rad / min, and the supernatant is taken for ultraviolet absorption measurement,...

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Abstract

The invention discloses a composite functional liposome and a preparation method and application thereof. The composite functional liposome comprises the following components: granulesten, cholesterin, vitamin C and salicylic acid, wherein the mass ratio of the four components is 5-7:1.3-1.5:0.8-1.5:1. The method for preparing the composite functional liposome comprises the following steps of: dissolving the granulesten, the cholesterin and the salicylic acid in an organic solvent to obtain a mixture serving as oil phase; emulsifying the mixture and vitamin C-containing buffer solution which serves as water phase to form water-in-oil microemulsion; emulsifying the water-in-oil microemulsion and buffer solution to finally form water / oil / water multiple emulsion, wherein the emulsification process is completed by ultrasonication; and removing the organic solvent through decompression and distillation to obtain the composite functional liposome. The composite functional liposome has stable property, contains fat-soluble medicaments and water-soluble medicaments, exerts the effects of various medicaments to compound the functions of the liposome, is applied to cosmetics, improves the stability of the medicaments, and reduces the production cost.

Description

technical field [0001] The invention relates to a preparation method of liposome, in particular to a composite functional liposome and its preparation method and application. Background technique [0002] Liposome was discovered and named by Dr. Bangham in 1965. Its structure dimension is a closed vesicle with a bimolecular membrane as a sandwich wall. Various lipids and lipid mixtures can be used to prepare liposomes, with phospholipids being the most commonly used. The main components of phospholipids are phosphatidylcholine, phosphatidylethanolamine, phosphatidylserine, phosphatidylglycerol, and phosphatidic acid. Phospholipids are the main components of cell membranes, so liposomes are similar in structure to human cells and have a high affinity for human cells. Phospholipids are amphiphilic molecules whose structure contains a hydrophilic group (a phosphate group and a nitrogenous base) and a hydrophobic group (two longer hydrocarbon chains). Under certain concentrat...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K9/127A61K47/28A61K47/24A61K47/22A61K47/12
Inventor 何秋星王学文
Owner GUANGDONG PHARMA UNIV
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