Simple and feasible extract method of edible mushroom genomic DNA

Abstract

The invention provides a simple and feasible extract method of edible mushroom genomic DNA, which comprises the following steps: selecting materials; grinding; adding buffer solution, and centrifuging after oscillation; standing and depositing; centrifuging; washing and depositing, and air-drying; and dissolving, depositing, preserving and the like. The method for extracting the edible mushroom genomic DNA has short extract time, simple operation, low cost and high extract efficiency.

Description

technical field [0001] The invention relates to a method for extracting genome DNA of edible fungi, which belongs to the field of biotechnology. Background technique [0002] With the rapid development of modern biotechnology, the study of molecular biology of edible fungi has also entered a new stage. The extraction, separation and purification of edible fungus genomic DNA are very necessary for the study of genetic engineering such as genetic evolution, gene composition, gene recombination and expression of edible fungi at the molecular level. Using a simple and easy extraction method to obtain the genome DNA from the edible fungus mycelium and fruiting body will greatly improve the work efficiency. [0003] The cell walls and chromosomes of edible fungi are combined with complex polysaccharides and rich proteins, and their mycelia usually produce pigments, so that the prepared genomic DNA contains different amounts of polysaccharides, proteins, pigments, and cell wall su...

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Problems solved by technology

Sun Lifu et al. (Acta Mycology, 2009, Volume 28, No. 2, 299-302) used a small manual electric drill with a sterile plastic drill bit to grind and successfully extracted the total DNA of Armillaria armillaria. Although this method avoids the use of liquid nitrogen, But there are still time-consuming shortcomings; Zhao Chunxi et al. (Anhui Agricultural Sciences, Volume 36, No. 28, 2008: 12122-12200) tried to use the improved TRIS saturated phenol one-step method to extract high-quality DNA from various edible mushroom fruiting bodies. , the edible fungus DNA quality that obtains is better, can satisfy follow-up molecular biology tests such as PCR, but still adopted in this method and utilizes the mortar grinding method, is difficult for the processing of a large number of samples; Yang Tongwen et al. Phase 1: 32-34) explored a simple and efficient method of CTAB binding DNA gel kit, eliminating the need for phenol-chloroform extraction, eliminating the steps of drying and re-dissolving DNA to convert CTAB The cleavage function is organically combined with the purification function of the gel recovery kit, and the combined operation steps are optimized to successfully extract the DNA of the fruiting bodies of Pleurotus ostreatus, Lentinus edodes, and Flammulina velutipes, but this method is costly

[0005] In summary, the current methods for extracting genomic DNA from edible fungi generally have disadvantages such as cumbersome operation, long extraction time, and high cost.

[0006] In addition, there are few reports on the extraction of nucleic acids from the fruiting bodies of edible fungi, which limits the rapid identification of existing cultivars and the research on the diversity of wild edible fungus resources at the molecular level

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