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Application of butyric acid producing beneficial bacterium in preparing preparation for preventing and treating severe disease gut barrier injury and post-injury complication

The technology of intestinal barrier and beneficial bacteria is applied in the application field of butyric acid-producing beneficial bacteria in the preparation of preparations for preventing and treating intestinal barrier damage and complications after damage in severe diseases, and can solve the problem that the proximal intestinal tract cannot be effectively achieved, and the unpleasant odor , poor dose control, etc.

Inactive Publication Date: 2010-10-06
QINGDAO EASTSEA PHARMA +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, if butyric acid is administered in the form of its free salt, it will produce an unpleasant smell, and it will be absorbed in the stomach and small intestine when it is administered orally, and cannot effectively reach the large intestine to play a role
Made into slow-release or enteric-coated preparations, the preparations are relatively expensive, and will be excreted quickly with intestinal peristalsis, and the dosage is not easy to control
Made into an enema, which is inconvenient to apply and cannot effectively reach the proximal intestinal tract
Therefore, the above various problems have restricted the application of butyric acid preparations in patients with severe diseases.

Method used

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  • Application of butyric acid producing beneficial bacterium in preparing preparation for preventing and treating severe disease gut barrier injury and post-injury complication
  • Application of butyric acid producing beneficial bacterium in preparing preparation for preventing and treating severe disease gut barrier injury and post-injury complication
  • Application of butyric acid producing beneficial bacterium in preparing preparation for preventing and treating severe disease gut barrier injury and post-injury complication

Examples

Experimental program
Comparison scheme
Effect test

preparation Embodiment 1

[0079] Preparation of Medicine Preparation Example 1 Oral Fungi Live Bacteria Powder

[0080] 1 Preparation of bacteria powder and identification of strains

[0081] Take healthy baby feces, then put the feces in a sterilized anaerobic bottle, blow in nitrogen gas and mix well at the same time, quickly take 2 grams of feces from it and add it to 18mL sterilized diluent, blow in nitrogen gas and mix well at the same time. In the console, do 10 -1 、10 -2 、10 -3 、10 -4 , 10 -5 , 10 -6 , 10 -7 Gradient dilution, take 10 -5 , 10 -6 , 10 -7 Three dilution gradients, spread on the Eubacterium selective single colony isolation solid medium, place in an anaerobic tank, anaerobic culture at 37°C for 48 hours, select four single colonies with good growth and inoculate them into the Eubacterium liquid The expansion medium was placed in an anaerobic tank, and the expansion culture was carried out at 37° C. for 48 hours in anaerobic condition. After the obtained culture solution ...

preparation Embodiment 2

[0098] Preparation of oral Clostridium tertiary live bacteria powder and oral Clostridium butyricum (Clostridium butyricum) live bacteria powder 1 preparation of bacteria powder and identification of strains

[0099] Take healthy baby feces, then put the feces in a sterilized anaerobic bottle, blow in nitrogen gas and mix well at the same time, quickly take 2 grams of feces from it and add it to 18mL sterilized diluent, blow in nitrogen gas and mix well at the same time. In the console, do 10 -1 、10 -2 、10 -3 、10 -4 , 10 -5 , 10 -6 , 10 -7 Gradient dilution, take 10 -5 , 10 -6 , 10 -7 Three dilution gradients, 80°C water bath for 13 minutes, spread on the solid medium for Clostridium selective single colony isolation, place in an anaerobic tank, culture at 37°C for 48 hours, and select three single colonies with good growth They were respectively inoculated into Clostridium liquid expansion medium, placed in an anaerobic tank, and amplified at 37° C. for 48 hours unde...

preparation Embodiment 3

[0115] Medicine Preparation Example 3 Preparation of Bifidobacterium and Lactobacillus Powder and Fermentation Supernatant

[0116] In order to further illustrate the novelty and creativity of the present invention, Bifidobacterium and Lactobacillus are used for comparative research in the application effect examples. The selected bifidobacterium is Bifidobacterium infantis DF96102 with preservation number CGMCC0313.2, and the lactobacillus is Lactobacillus acidophilus DF96106 with preservation number CGMCC0313.4.

[0117] 1 Preparation of Bifidobacteria powder and fermentation supernatant

[0118] Culture medium is (medium is not limited to that described in the present invention, known culture medium all can): peptone 15.0g, yeast powder 2.0g, glucose 20.0g, soluble starch 0.5g, sodium chloride 5.0g, 5% cysteine Amino acid 10.0mL, tomato extract 400.0mL, Tween 80 1.0mL, liver extract 80.0mL, agar 20.0g, distilled water 520.0mL, adjust pH to 7.0, sterilize at 115°C for 20min...

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Abstract

The invention relates to a new use of a butyric acid producing beneficial bacterium, in particular to one or more applications of the butyric acid producing beneficial bacterium in preparing a preparation for preventing and treating a severe disease gut barrier injury and post-injury complication, wherein the prevention and treatment of the gut barrier injury and post-injury complication of the severe disease is a prevention an treatment function on the gut barrier which is realized by producing the butyric acid in the intestinal tract, introducing the butyric acid into the intestinal tract, providing intestinal mucosa energy, promoting cell proliferation of the intestinal mucosa, restricting the cell apoptosis of the intestinal mucosa, improving the permeability of the intestinal mucosa and preventing and curing the intestinal mucosa carrier.

Description

technical field [0001] The invention relates to the application of one or more beneficial bacteria producing butyric acid in the preparation of preparations for preventing and treating intestinal barrier damage and post-injury complications in severe diseases, specifically refers to the beneficial bacteria producing butyric acid produce butyric acid in the intestinal tract, Butyric acid is introduced into the intestinal tract to provide intestinal mucosal energy, promote intestinal mucosal cell proliferation and inhibit intestinal mucosal cell apoptosis, improve intestinal mucosal permeability, prevent and treat intestinal mucosal barrier damage and play a role in the prevention and treatment of intestinal barrier. It belongs to biological drugs field. Background technique [0002] A large number of studies have shown that patients with severe diseases, including severe trauma, shock, burns, surgery, severe infection, critically ill patients, patients admitted to intensive c...

Claims

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Application Information

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IPC IPC(8): A61K35/74A61P1/00A61P3/02A23L1/29A23K1/16A23L33/00
Inventor 崔云龙李洪福
Owner QINGDAO EASTSEA PHARMA
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