Method for producing high-concentration D-lactic acid by adopting synchronous enzymolysis and fermentation on peanut meal and special culture medium thereof

A fermentation medium and simultaneous enzymatic hydrolysis technology are applied in the methods of using bacteria, microorganism-based methods, biochemical equipment and methods, etc., to achieve the effects of simple ingredients, low cost, and saving site occupation and equipment investment.

Active Publication Date: 2010-11-17
TIANJIN INST OF IND BIOTECH CHINESE ACADEMY OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Peanut meal, as another cheap nitrogen source with wide distribution, high yield and rich nitrogen content, has not been reported for the fermentative production of D-lactic acid

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] Example 1 D-lactic acid was produced by fermentation at 37° C. with Sporolactobacillus inulinus CASD CGMCC No. 2185, 50 mL fermentation broth / 100 mL Erlenmeyer flask, and the fermentation time was 42 hours.

[0038] The composition of each culture medium used in the present embodiment is as follows:

[0039] Incline medium: glucose 10 g / l, yeast powder 5 g / l, calcium carbonate 1 g / l, agar 20 g / l, solvent is water, the pH of the slant medium is 6.5, sterilized at 121°C for 20 minutes .

[0040] Seed culture medium: glucose 40 g / L, yeast powder 5 g / L, calcium carbonate 3 g / L, solvent is water, pH of the seed culture medium is 6.5, sterilized at 121° C. for 20 minutes.

[0041] Fermentation medium: industrial glucose 135 g / L, peanut meal 40 g / L, neutral protease 0.1 g / L, calcium carbonate 70 g / L, the balance is water, the pH of the fermentation medium is 6.5, 115°C Sterilize for 20 minutes.

[0042] The method for producing D-lactic acid by fermentation comprises the fo...

Embodiment 2

[0047] Example 2 D-lactic acid was produced by fermentation at 42° C. with Sporolactobacillus inulinus CASD CGMCC No. 2185, 50 mL fermentation broth / 100 mL Erlenmeyer flask, and the fermentation time was 42 hours.

[0048] The composition of each culture medium used in the present embodiment is as follows:

[0049] The slant medium, seed medium and fermentation medium are the same as in Example 1.

[0050] The method for producing D-lactic acid by fermentation comprises the following steps:

[0051] (1) inclined plane culture: with embodiment 1;

[0052] (2) seed culture: with embodiment 1;

[0053] (3) Fermentation culture: Inoculate the above-mentioned seed medium into the fermentation medium (50mL fermentation broth / 100mL Erlenmeyer flask) with an inoculation amount of 10% by volume, place it at 42°C for static cultivation, and shake and stir once every 12 hours , cultivated for 42 hours, and ended the fermentation.

[0054] After the fermentation, according to the dete...

Embodiment 3

[0055] Example 3 Using Sporolactobacillus inulinus (Sporolactobacillus inulinus) CASD CGMCC No. 2185 to ferment and produce D-lactic acid at 47°C, 50mL fermentation broth / 100mL Erlenmeyer flask, the fermentation time was 42 hours.

[0056] The composition of each culture medium used in the present embodiment is as follows:

[0057] The slant medium, seed medium and fermentation medium are the same as in Example 1.

[0058] The method for producing D-lactic acid by fermentation comprises the following steps:

[0059] (1) inclined plane culture: with embodiment 1;

[0060] (2) seed culture: with embodiment 1;

[0061] (3) Fermentation culture: Inoculate the above-mentioned seed medium into the fermentation medium (50mL fermentation broth / 100mL Erlenmeyer flask) with an inoculation amount of 10% by volume, place it at 47°C for static cultivation, and shake and stir once every 12 hours , cultivated for 42 hours, and ended the fermentation.

[0062] After the fermentation is fi...

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Abstract

The invention discloses a method for producing high-concentration D-lactic acid by adopting synchronous enzymolysis and fermentation on peanut meal and a special culture medium thereof. The special culture medium adopts peanut meal as a fermenting culture medium of an organic nitrogen source. In addition, protease is added into the special culture medium. In the method for producing the high-concentration D-lactic acid, Sporolactobacillus inulinus CASD CGMCC (Computer Aided System Design China General Microbiological Culture Collection center) No.2185 is inoculated to the special culture medium for fermenting to obtain the high-concentration D-lactic acid. The method for producing the high-concentration D-lactic acid of the invention is obviously better than the traditional method for producing the high-concentration D-lactic acid, has the advantages of high purity, high production efficiency, low requirement on instrument and equipment, low cost, and the like and is suitable for large-area popularization and application.

Description

technical field [0001] The invention belongs to the technical field of fermentation engineering, in particular to a method for producing high-concentration D-lactic acid by synchronous enzymatic hydrolysis and fermentation of peanut meal and a special culture medium thereof. Background technique [0002] Lactic acid, the scientific name is α-hydroxypropionic acid (d-hydroxy-propionic acid), and the chemical molecular formula of lactic acid is C 2 h 5 OCOOH, is a simple hydroxy acid. There is an asymmetric carbon atom in the lactic acid molecule, so lactic acid has optical activity. L-lactic acid is levorotatory, D-lactic acid is dextrorotatory, and DL-lactic acid is racemic. In recent years, polylactic acid, a derivative of lactic acid, has attracted widespread attention. Its physical properties are very similar to polystyrene. In addition to its excellent characteristics of plastic materials based on petrochemicals, it also has the biggest feature of being biodegradable....

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P7/56C12P21/06C12R1/01
CPCC12P7/56C12R1/01C12N1/205C12R2001/01
Inventor 许平赵博李峰嵩王丽敏马延和马翠卿李庆刚唐鸿志
Owner TIANJIN INST OF IND BIOTECH CHINESE ACADEMY OF SCI
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