Method for separating and extracting apigenin, acacetin-7-O-beta-D-glucoside and apigenin-7-O-beta-D-glucoside from dendranthema indicum

Abstract

The invention discloses a method for separating and extracting apigenin, acacetin-7-O-beta-D-glucoside and apigenin-7-O-beta-D-glucoside from dendranthema indicum, which belongs to a method for extracting chemical substances from a natural plant. The method is sequentially carried out according to the following steps of: 1. extract preparation; 2. extraction: sequentially extracting the extract of the dendranthema indicum by using petroleum ether, chloroform and ethyl acetate; and 3. separation and extraction: (1) carrying out normal-pressure and reduced-pressure silica column chromatography on the extracted part by using the ethyl acetate; and (2) separating fractions through a semi-preparative high-efficiency liquid chromatographic instrument to obtain the acacetin-7-O-beta-D-glucoside, the apigenin-7-O-beta-D-glucoside and the apigenin as compounds. In the method, the semi-preparative high-efficiency liquid chromatographic instrument is directly adopted for separation to obtain the three compounds after silica column chromatography is adopted, and the method has good separation effect, short time, high product purity and less environmental pollution.

Description

technical field [0001] The invention belongs to a method for extracting chemical substances from natural plants, in particular to apigenin, locustin-7-O-β-D-glucoside, apigenin-7-O- β-D-glucoside method. Background technique [0002] Shennongxiangju Dendranthema indicum (L.) Des Monl.var aromaticum Q.H.Liuet S.F.Zhang. is a new variety of Dendranthema in the family Asteraceae. It is a unique plant in Shennongjia. It is produced in Shennongjia forest area, Hubei Province, distributed at an altitude of 1200 meters Above the sunny open hillsides, roadsides, rock crevices, and open spaces beside bushes, the whole plant has a special strong aroma, and folks use its flowers and leaves dried in the shade as sachets. In the mountainous area of ​​Western Hubei, Shennong Xiangju is used to prevent and treat colds, headaches, bacillary dysentery, enteritis, constipation, coronary heart disease and high blood pressure. It can be used to extract essential oils or extracts for use in me...

AI Technical Summary

Problems solved by technology

However, there are few studies on the chemical substance basis of Shennongxiangju at present. In the existing reports on the chemical components of Shennongxiangju, there is no discovery of apigenin, locustin-7-O-β-D-glucoside, celery A report on the prime-7-O-β-D-glucoside

The existing separation of apigenin, locustin-7-O-β-D-glucoside and apigenin-7-O-β-D-glucoside in Compositae phytochemical components, especially the separation of chemical components of Shennong chrysanthemum Most of the methods use repeated silica gel elution, and then use other types of expensive stationary phases and column chromatography that consumes a lot of organic reagents to separate the compounds. This method is more polluting to the environment.

For example, in the study of separation, purification and structure determination of flavonoid active ingredients in chamomile by Yang Yansong et al., apigenin-7-O-β-D-glucoside was obtained through repeated polyamide column chromatography and purification. Long cycle, large consumption of reagents, high cost and great environmental pollution

Gu Yaohua et al. used repeated silica gel column chromatography to separate apigenin and apigenin-7-O-β-D-glucoside from Boju. Great environmental pollution

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