Microecological microbial agent for resisting banana vascular wilt and preparation method thereof

A microbial agent, a technology for banana fusarium wilt, which is applied in the field of microbial fermentation to achieve the effects of reducing costs, simplifying links, and high resistance to banana fusarium wilt

Inactive Publication Date: 2010-12-15
INST OF SOIL & FERTILIZER FUJIAN ACADEMY OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the research on the preparation of biocontrol agents with Bacillus subtilis to prevent and control plant diseases has become a research hotspot at home and abroad. There are many reports on the application of Bacillus subtilis in plant biological control, but there are few studies on fermentation conditions.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] 1. Preparation of Bacillus subtilis fermentation medium

[0032] 0.5% molasses, 1% beef extract, 1000ml water; pH7.5; 55ml / 250ml triangular bottle volume, autoclaved at 121°C for 30min.

[0033] 2. Preparation of Bacillus subtilis seed solution

[0034] Activate the Bacillus subtilis CS12 strain (stored in the Agricultural Microbiology Laboratory of the Soil and Fertilizer Research Institute of the Fujian Academy of Agricultural Sciences) for 24 hours on a bacterial culture slope, pick two rings and inoculate it into a 50ml / 250ml Erlenmeyer flask bacterial liquid medium, place Shake culture at 28°C and 160r / min for 24h.

[0035] 3. Bacillus subtilis shake flask fermentation

[0036] The CS12 seed solution was inoculated into the fermentation medium (5% inoculum), and cultured with shaking at 30°C and 180r / min for 50h.

[0037] 4. After the fermentation is completed, the fermentation broth is collected.

[0038] 5. Detection

[0039] Biomass was determined by plate ...

Embodiment 2

[0043] 1. Preparation of Bacillus subtilis fermentation medium

[0044] 0.5% corn flour, 1% soybean meal, 1000ml water; pH7.0; 50ml / 250ml triangle bottle, autoclaved at 121°C for 30min.

[0045] 2. Preparation of Bacillus subtilis seed solution

[0046] Activate the Bacillus subtilis CS18 strain (stored in the Agricultural Microbiology Laboratory of the Soil and Fertilizer Research Institute of the Fujian Academy of Agricultural Sciences) for 24 hours on a bacterial culture slope, pick two rings and inoculate it into a 50ml / 250ml Erlenmeyer flask bacterial liquid medium, place in Shake culture at 30°C and 180r / min for 24h.

[0047] 4. Bacillus subtilis shake flask fermentation

[0048] The CS18 seed liquid was inoculated into the fermentation medium (the inoculum size was 4%), and cultured with shaking at 30°C and 180r / min for 10h.

[0049] 5. After the fermentation is completed, the fermentation broth is collected.

[0050] 6. Detection

[0051] Biomass was determined by...

Embodiment 3

[0054] 1. Preparation of Bacillus subtilis fermentation medium

[0055] Sucrose 0.4%, soybean powder 1.2%, water 1000ml; pH7.6; 45ml / 250ml triangle bottle, autoclaved at 121°C for 30min.

[0056] 2. Preparation of Bacillus subtilis seed solution

[0057] Activate the Bacillus subtilis CS16 strain (stored in the Agricultural Microbiology Laboratory of the Soil and Fertilizer Research Institute of the Fujian Academy of Agricultural Sciences) for 24 hours on a bacterial culture slope, pick two rings and inoculate it into a 50ml / 250ml Erlenmeyer flask bacterial liquid medium, place in Shake culture at 30°C and 180r / min for 28h.

[0058] 3. Bacillus subtilis shake flask fermentation

[0059] The CS16 seed solution was inoculated into the fermentation medium (inoculum size 6%), and cultured with shaking at 20°C and 200r / min for 25h.

[0060] 4. After the fermentation is completed, the fermentation broth is collected.

[0061] 5. Detection

[0062] Biomass was determined by plate ...

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Abstract

The invention provides a microecological microbial agent for resisting banana vascular wilt and a preparation method thereof. A fermentation medium for the fermentation liquor of the microecological microbial agent for resisting the banana vascular wilt is prepared from the following components in percentage by weight: 0.1 to 10 percent of corn flour, starch, cane sugar or molasses, 1 to 10 percent of bean pulp, soybean meal, bran or beef extract and 1,000ml of water under the condition of the pH value of 6 to 9. The method comprises the following steps of: preparing bacillus subtilis seed liquor; inoculating the prepared bacillus subtilis seed liquor to the fermentation medium for culturing; and collecting the bacillus subtilis fermentation liquor after the fermentation is finished. In the microbial agent and the preparation method, the raw materials of the fermentation medium are economic and cheap; and the fermentation medium has a proper concentration, inoculation quantity and bottling quantity, so that the fermented product has higher activity for resisting the banana vascular wilt. The formula of the fermentation medium and the fermentation condition can make a fermentation product have high antibacterial activity and a low cost. The adopted detection method is fast and convenient and a straightforward detection result can be obtained by the detection method.

Description

technical field [0001] The invention belongs to the field of microbial fermentation, and in particular relates to an anti-banana fusarium wilt micro-ecological bacterial agent and a preparation method thereof. Background of the invention [0002] Banana wilt is known as the "cancer" of bananas and is a typical soil-borne disease. The pathogen is Fusarium oxysporum ( Fusarium oxysporum f.sp. cubense, FOC). Due to the unsatisfactory effect of chemical control, easy to produce resistance, and pollution of the environment, biological control of Fusarium wilt of banana has gradually become a new direction for the control of Fusarium wilt. [0003] Bacillus subtilis is a mesophilic, aerobic spore-forming Gram-positive rod-shaped bacterium, which is widely distributed, easy to isolate and cultivate, non-toxic and harmless to humans and animals, does not pollute the environment, and can produce heat-resistant and stress-resistant bacteria. spores. At present, the fungus has sh...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N63/02A01P3/00C12N1/20C12R1/125
Inventor 陈济琛林新坚林戎斌林陈强白丹红蔡海松张慧
Owner INST OF SOIL & FERTILIZER FUJIAN ACADEMY OF AGRI SCI
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