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Method for producing biological oil by sweet potato full-matrix fermentation

A bio-oil and sweet potato technology, applied in the biological field, can solve the problems of high oil cost and immature technology, and achieve the effects of reducing production costs, reducing high-temperature gelatinization, and obvious industrialization advantages

Inactive Publication Date: 2011-01-19
SOUTHWEST UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the cost of using high-quality carbon sources such as glucose and corn starch as raw materials to ferment and produce oil is relatively high; while the technology for oil and fat fermentation production using fibrous non-grain raw materials is still immature and still in the development stage

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0015] Adopt sweet potato whole matrix synchronous saccharification and fermentation process (sterilization): Weigh sweet potato powder and 3% sodium hydroxide and mix with a material-liquid mass-volume ratio of 1:6, hydrolyze for 5 hours, and adjust the pH to 6.0. The hydrolyzate was dispensed into 250 mL Erlenmeyer flasks with a liquid volume of 50 mL, and the hydrolyzate was sterilized at 121 °C for 30 min. After cooling, add 50 units (per gram of starch) of α-amylase and 200 units (per gram of starch) of glucoamylase, and at the same time add 10% (V / V) yeast seed solution (the strain is purchased from China General Microorganism Culture Collection Management Center (CGMCC) oleaginous yeast strains (Tychosporium dermatosa Trichosporon cutaneum CGMCC 2.1374), the culture condition is 29°C, the shaker speed is 180 r / min, and cultured for 96h.

[0016] Then use the acid-heat method to extract oil: take the fermented liquid and centrifuge it at 4000 r / min, add 4mol / L hydrochl...

Embodiment 2

[0018] Synchronous saccharification and fermentation process of sweet potato whole matrix (without sterilization): Weigh sweet potato powder and 3% sodium hydroxide and mix them at a mass-volume ratio of 1:6, hydrolyze for 5 hours, and adjust the pH to 6.0. Divide the hydrolyzate into 250 mL Erlenmeyer flasks, the liquid volume is 50 mL, the hydrolyzate is not sterilized, add 50 units (per gram of starch) of α-amylase and 200 units (per gram of starch) of Glucoamylase, while adding 10% (V / V) yeast seed solution (the strain was purchased from the China General Microorganism Culture Collection Center (CGMCC) oleaginous yeast strain (Tychosporium dermatosa Trichosporon cutaneum CGMCC 2.1374), the culture condition is 29°C, the shaker speed is 180 r / min, and cultured for 96h.

[0019] Use the acid-heat method to extract oil: take the fermentation broth and centrifuge at 4500 r / min, add 5 mol / L hydrochloric acid at a rate of 5 ml per gram of bacteria, oscillate and mix, place at r...

Embodiment 3

[0021] Synchronous saccharification and fermentation process of sweet potato all-matrix raw material (without sterilization): Weigh an appropriate amount of sweet potato powder and mix it with sterile water. Divide the sweet potato mixture into 250 mL Erlenmeyer flasks with a liquid volume of 50 mL without sterilization, add 50 units (calculated per gram of starch) of α-amylase and 200 units (calculated per gram of starch) of saccharification Enzymes, while adding 10% (V / V) yeast seed solution (purchased from China General Microorganism Culture Collection Center (CGMCC) oleaginous yeast strain (Tychosporium dermatosa Trichosporon cutaneum CGMCC 2.1374), the culture condition is 29°C, the shaker speed is 180 r / min, and cultured for 96h.

[0022] Use the acid-heat method to extract oil: take the fermented liquid and centrifuge it at 5000 r / min, add 4mol / L hydrochloric acid at a ratio of 8ml per gram of bacteria to the bacterial sediment, oscillate and mix, place it at room temp...

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Abstract

The invention provides a method for producing biological oil by sweet potato full-matrix fermentation, comprising the following steps: adding dried potato powder or fresh sweet potato and sodium hydroxide with the concentration of 3% based on the material-liquid mass-volume ratio of 1:5-10, hydrolyzing for 5-10 hours and adjusting pH to 5.0-6.0; adding the obtained hydrolysate to 40-80u / g of alpha-amylase and 150-300u / g of glucoamylase in terms of per gram of starch and meanwhile inoculating 5-15% (V / V) of oleaginous yeast seed liquid for simultaneous saccharification and fermentation, wherein, shake-flask fermentation control conditions of oleaginous yeast are as follows: the inoculation quantity is 5-15%, the culture temperature is 26-30 DEG C, the revolving speed of a shaker is 180-220r / min and the fermentation time is 3-6 days; and finally extracting the oil. In the method, by a sweet potato simultaneous saccharification and fermentation process, a cheap and available sweet potato resource is taken as a raw material without adding other culture medium components, thus reducing energy consumption during the production process and lowering production cost. The biological oil composition obtained by microbes is similar to that of vegetable oil and can be taken as the raw material for producing biodiesel; and compared with other process routes, the method of the invention has obvious industrialization advantages.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a method for producing bio-oil by fermenting sweet potato raw materials with oleaginous yeast. Background technique [0002] In recent years, as a substitute for fossil energy, biodiesel is the most important renewable liquid fuel product, and has become the fastest growing and most widely used environmentally friendly renewable energy in the world. The traditional method of biodiesel is obtained by transesterification reaction of vegetable oil (soybean, rapeseed, jatropha, etc.), waste cooking oil, etc., and vegetable oil is used as raw material to produce biodiesel. %. The prices of rapeseed oil, soybean oil, cottonseed oil, industrial waste fatty acids, and kitchen waste oil used as raw materials for biodiesel are higher than or close to those of biodiesel products. Low-cost supply issues. [0003] Under certain conditions, certain microorganisms can convert carbon ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P7/64C12P19/14C12R1/645
CPCY02E50/13Y02E50/10
Inventor 邹祥付玉凡杨琼丹
Owner SOUTHWEST UNIVERSITY
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