Method for preparing chitin, L-calcium lactate and compound amino acid or peptide from carapace waste

A waste, chitin technology, applied in biochemical equipment and methods, microorganism-based methods, microorganisms, etc., can solve the problems of low calcium salt removal rate, low acid production rate, high cost, and achieve high decalcification rate. , the effect of high purity

Inactive Publication Date: 2011-02-02
HUBEI UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the method of this patent adopts any one or three mixed bacteria of Lactobacillus acidophilus, Streptococcus thermophilus and Lactobacillus delbrueckii subsp. bulgaricus to extract protein and chitin from shrimp heads and shells. It is a heteromorphic lactic acid fermentation, the acid production rate is not high, and the removal rate of calcium salt is low after fermentation, so that the residue needs to be treated with dilute acid (0.6mol / L) to completely remove calcium salt to obtain chitin, which not only leads to the loss of calcium salt , will also cause environmental pollution, and the lactic acid produced by fermentation (so-called high-acid environment) will cause protein autolysis under the existing fermentation conditions, so that the possibility of fully hydrolyzing protein is extremely small
In addition, the method has a long fermentation period (5-7 days), and its cost is high, and the fermentation process is difficult to control

Method used

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  • Method for preparing chitin, L-calcium lactate and compound amino acid or peptide from carapace waste

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] Example 1 Preparation of chitin from crustacean waste such as shrimp and crab

[0026] 1. Clean production process and parameters

[0027] 1) Cleaning of crustacean waste such as shrimp and crab: clean the soil and other dirt on the crustacean waste such as shrimp and crab with tap water, dry and pulverize;

[0028] 2) Grinding fineness: 20-60 mesh.

[0029] 2. Solid-state fermentation decalcification and degreasing of crustacean waste

[0030] 1) Cultivation of Lactobacillus rhamnosus and Bacillus subtilis seeds

[0031] 1.1) Lactobacillus rhamnosus culture medium

[0032] A, composition of slant activation medium (g / 100ml): peptone 1%, beef extract 1%, yeast extract 0.5%, glucose 2%, diammonium hydrogen citrate 0.2%, potassium dihydrogen phosphate 0.2%, Tween 800.1% , magnesium sulfate 0.058%, manganese sulfate 0.025%, sodium acetate 0.2%, agar 2%, calcium carbonate 0.6%, sterilized at 115°C for 30min, pH 6.4.

[0033] B. Composition of liquid seed medium (g / 100m...

Embodiment 2

[0066] Example 2 Preparation of L-calcium lactate, compound amino acids or peptides from crustacean waste such as shrimp and crabs

[0067] 1. Clean production process and parameters

[0068] 1) Cleaning of crustacean waste such as shrimp and crab: clean the soil and other dirt on the crustacean waste such as shrimp and crab with tap water, dry and pulverize;

[0069] 2) Grinding fineness: 20-60 mesh.

[0070] 2. Solid-state fermentation decalcification and degreasing of crustacean waste

[0071] 1) Cultivation of Lactobacillus rhamnosus and Bacillus subtilis seeds

[0072] 1.1) Lactobacillus rhamnosus culture medium

[0073] A, composition of slant activation medium (g / 100ml): peptone 1%, beef extract 1%, yeast extract 0.5%, glucose 2%, diammonium hydrogen citrate 0.2%, potassium dihydrogen phosphate 0.2%, Tween 800.1% , magnesium sulfate 0.058%, manganese sulfate 0.025%, sodium acetate 0.2%, agar 2%, calcium carbonate 0.6%, sterilized at 115°C for 30min, pH 6.4.

[0074] ...

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Abstract

The invention relates to a method for preparing chitin, L-calcium lactate and compound amino acid or peptide by performing microbial fermentation on carapace waste of shrimps, crabs and the like. The method comprises the following steps of: fermenting the carapace waste in seed solution of Lactobacillus rhamnosus CICC6003 and Bacillus subtilis CICC23579 to obtain the L-calcium lactate; and fermenting the carapace waste in the seed solution of Aspergillus oryzae CICC41477 to obtain the compound amino acid or the peptide and the chitin. By the method, decalcification, degreasing and deproteinization of the carapace waste of the shrimps, the crabs and the like can be realized, and high-quality chitin, L-calcium lactate and compound amino acid (peptide) series products can be prepared so as to realize comprehensive utilization of protein and calcium carbonate resources.

Description

technical field [0001] The invention relates to a method for preparing chitin, L-calcium lactate and compound amino acids or peptides by using crustaceous waste, in particular to a method for preparing chitin, L-calcium lactate and compound amino acids or peptides by microbial fermentation of crustacean waste such as shrimp and crabs. peptide method. Background technique [0002] Chitin is the second largest biological polysaccharide after cellulose on the earth, and it is also the only natural alkaline polysaccharide on the earth. It is widely found in the shells of crustaceans, shrimps and crabs, the shells of insects, fungi (including yeast, mold, etc.) etc.), cell walls of algae and plants. The annual biosynthesis of chitin is about 10 billion tons. [0003] The product of deacetylation of chitin is chitosan. The molecular structure of chitosan contains active groups such as amino groups, hydroxyl groups, acetamido groups, oxygen bridges, and electron-rich pyran rings...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P7/56C12R1/225C12R1/69C12P19/04C12P21/00C12R1/125C12P39/00
Inventor 蔡俊王常高胡瑛
Owner HUBEI UNIV OF TECH
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