Method for extracting aucubin from aucuba japonica leaves

A technology for aucubin and aucubin, which is applied in the field of extracting aucubin by using macroporous resin and membrane separation technology, can solve the problems of large amount of organic reagents, high cost, only suitable for scientific research experiments, etc. Less, less harm, better effect

Inactive Publication Date: 2011-02-16
NANJING ZELANG AGRI DEV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0011] Chinese patent (Application No. 200810017377.2) "A method for extracting aucubin from Artemisia annua plants", the process uses ether degreasing, methanol cold immersion, acetone crystallization, ethanol-methanol recrystallization, the method uses a large amount of organic reagents

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  • Method for extracting aucubin from aucuba japonica leaves

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Embodiment 1

[0025] Pulverize the leaves of Peach Coralia to 20 meshes, put 5kg into a microwave extraction tank, add 25L of 95% ethanol solution for microwave extraction for 1 hour, the power of microwave extraction is 1kW, the extraction temperature is controlled at 40-50°C, extract 3 times, and combine the extracts to reduce Recover the ethanol by pressure until the extract is dissolved, add water to dissolve, filter out the insoluble matter, add the filtrate to a 5L AB-8 macroporous resin column for adsorption, wash with water until the sugar reaction is negative, then elute the active ingredient with 20L of 70% ethanol solution, and collect the eluate , the eluate is added to the hollow composite membrane ultrafiltration membrane with a molecular weight cut off of 3000 for ultrafiltration, and the permeate is added to a hollow composite nanofiltration membrane with a molecular weight cut off of 100 to concentrate. The inlet pressure is controlled at 0.4-0.6Mpa to obtain a concentrated s...

Embodiment 2

[0027] Pulverize the leaves of Peach Coralia to 80 mesh, take 5kg and put it into a microwave extraction tank, add 75L80% ethanol solution for microwave extraction for 2 hours, the power of microwave extraction is 3kW, the extraction temperature is controlled at 40-50°C, extract 2 times, combine the extracts to reduce Press to recover the ethanol until the extract is dissolved, add water to dissolve, filter out the insoluble matter, add the filtrate to a 5L NAK-II macroporous resin column for adsorption, wash with water until the sugar reaction is negative, then use 35L of 50% ethanol solution to elute the active ingredients, and collect the eluate , the eluate is added to the hollow composite membrane ultrafiltration membrane with a molecular weight cut-off of 6000 for ultrafiltration, and the permeate is added to a hollow composite nanofiltration membrane with a molecular weight cut-off of 100 to concentrate, and the inlet pressure is controlled to 0.5-0.7Mpa to obtain a conce...

Embodiment 3

[0029] Pulverize the leaves of Peach Coralia to 60 mesh, put 5kg into a microwave extraction tank, add 50L90% ethanol solution for microwave extraction for 1 hour, the power of microwave extraction is 4kW, the extraction temperature is controlled at 40-50°C, extract 3 times, combine the extracts to reduce Recover the ethanol by pressure until the extract is dissolved, add water to dissolve, filter out the insoluble matter, add the filtrate to a 5L ADS-7 macroporous resin column for adsorption, wash with water until the sugar reaction is negative, then elute the active ingredient with 30L of 60% ethanol solution, and collect the eluate , the eluate is added to ultrafiltration with a hollow composite membrane with a molecular weight cut off of 6000, and the permeate is added to a hollow composite nanofiltration membrane with a molecular weight cut off of 100 to concentrate, and the inlet pressure is controlled at 0.6-0.8Mpa to obtain a concentrated solution, and 5 times the amount...

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Abstract

The invention relates to a method for extracting aucubin from aucuba japonica leaves, which comprises the following steps of taking the aucuba japonica leaves as a raw material; extracting for 2 to 3 times by utilizing ethanol in a microwave mode; recovering the ethanol from an extracting solution; adding water in extract to be dispersed and filtered; adding a polarity macroporous resin column to absorb; eluting with 50 to 70% of the ethanol; ultra-filtering eluent by utilizing an ultra-filtration membrane; concentrating by utilizing a nano-filtration membrane; extracting a concentrated solution by adding ethyl acetate; concentrating and crystallizing the extracting solution; and washing crystals with chloroform and petroleum ether and drying in a low temperature and vacuum conditions. The process of the invention has the advantages of simple operation, low energy consumption, high extraction efficiency, the obtained product has high content, and the temperature in the process of production is easy to control. The method of the invention is easy to product in industrialization.

Description

Technical field: [0001] The present invention relates to a method for extracting aucubin from leaves of coral coral, in particular to a method for extracting aucubin by adopting macroporous resin and membrane separation technology. Background technique: [0002] Aucubin is an iridoid glycoside. [0003] Molecular formula: C 15 h 22 o 9 ; [0004] Molecular weight: 346.33; [0005] Molecular formula: [0006] [0007] Physical and chemical properties: Melting point 181°C (ethanol-ether), easily soluble in water, methanol, soluble in ethanol, insoluble in ether, chloroform, benzene and petroleum ether. [0008] Aucubin is widely found in plants, especially in the genus Aucubin, which has the highest content. Pharmacological studies have shown that aucubin has the functions of clearing damp heat, diuresis, analgesia, lowering blood pressure, protecting liver and liver, and anti-tumor. [0009] Aucubin Aucubin has a bisacetal structure in its molecular structure, whic...

Claims

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Application Information

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IPC IPC(8): C07H1/08C07H17/04
Inventor 苏刘花
Owner NANJING ZELANG AGRI DEV
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