Method for preparing bio-based nylon polybutyrolactam
A polybutyrolactam, bio-based nylon technology, applied in the directions of microorganism-based methods, biochemical equipment and methods, microorganisms, etc., can solve the limitation of the application of PA4, the high cost of PA4, and the high cost of butyrolactam production. and other problems to achieve the effect of reducing production costs
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Embodiment 1
[0022] All percentages in this embodiment are mass percentages (w / w) if not otherwise specified
[0023] (1) Fermentation of glutamic acid, strain: Corynebacterium glutamicum
[0024] Seed medium: glucose 2.5%, corn steep liquor 3.0%, urea 0.8%, KH 2 PO 4 0.1%, MgSO 4 0.05%, pH 7.0, temperature 35°C, culture until OD ≥ 1.0, pH around 7.2, residual sugar ≤ 0.8%, fermentation medium: glucose 8.0%, corn steep liquor 0.2%, urea 0.1%, KH 2 PO 4 0.2%, MgSO 4 0.05%, antifoaming agent 0.05%, pH7.0, supplement sugar during the fermentation process, use liquid ammonia to adjust the pH, and control the pH at 7.0.
[0025] During the fermentation process, take 500mL of mature seeds and insert them into a 5L fermenter with 2.0L fermentation medium, control the stirring speed to 600rpm, the air volume to 0.8vvm, and cultivate at 35°C for 6 hours. If the dissolved oxygen is lower than 10% during the process, increase The air volume keeps the dissolved oxygen ≥ 10%. After 6 hours, the c...
Embodiment 2
[0037] Different carbon sources were used to carry out glutamic acid fermentation shake flask experiments, which proved that different carbon sources produced glutamic acid.
[0038] Strain: Corynebacterium glutamicum, seed medium: glucose 2.5%, corn steep liquor 3.0%, urea 0.8%, KH 2 PO 4 0.1%, MgSO 4 0.05%, pH 7.0, temperature 35°C, culture until OD ≥ 1.0, pH around 7.2, residual sugar ≤ 0.8%, can be replanted, fermentation shake flask medium: carbon source 6.0%, corn steep liquor 0.2%, urea 0.1 %, KH 2 PO 4 0.2%, MgSO 4 0.05%, CaCO 3 1.0%, pH7.0. During the fermentation process, 10 mL of mature seeds were taken and inserted into a 500 mL shaker flask containing 40 mL of fermentation medium, cultured at 200 rpm, 35°C for 36 hours, and the content of glutamic acid was measured. The results are shown in Table 1.
[0039] Table 1
[0040] carbon source
Embodiment 3
[0042] Get the Sodium Glutamate 600g that buys on the market, be mixed with 6% Sodium Glutamate solution, step 2 obtains 200mL centrifugation of fermented liquid, collect thalline, add thalline in 6% Sodium Glutamate solution, Transform at 35°C for 2 hours, until no bubbles are released and the reaction reaches the end, and L-sodium glutamate is converted into γ-aminobutyric acid.
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