Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Method for preparing hydrogen by fermentation through using special anaerobic clostridium pasteurianum

A technology of anaerobic bacteria and fermentation substrate, applied in the field of hydrogen production by using efficient anaerobic bacteria, can solve problems such as difficult promotion, weak screening research, anaerobic fermentation conditions, etc.

Active Publication Date: 2014-07-02
SOUTHEAST UNIV
View PDF1 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Through patent search, the existing patents are mainly research on fermentation substrates, fermentation equipment and improving hydrogen production efficiency, such as a kind of sludge and organic waste mixed biological production Hydrogen method (application number: 200710032658.0 Zhou Shaoqi, etc.), photosynthetic microbial hydrogen production reactor (application number: 200620032282.4), biological fermentation hydrogen production device (application number: 00231651.X Shen Jianquan), a CO2 sub>The method of rapidly multiplying algae and directly using it for biological hydrogen production (200710010804.X) and the expanded bed equipment for high-efficiency fermentation biological hydrogen production (application number: 03260012.7, Ren Nanqi, etc.), etc., and for fermentation strains, especially high-efficiency hydrogen-producing bacteria The screening research on species is relatively weak, and there is only one biohydrogen production method and its special bacteria (application number: 200910088408.8 Xing Xinhui, etc.), but the bacteria used in this method are recombinant bacteria after gene introduction, the operation is more complicated, and the fermentation conditions require Strictly anaerobic, not easy to promote

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Method for preparing hydrogen by fermentation through using special anaerobic clostridium pasteurianum
  • Method for preparing hydrogen by fermentation through using special anaerobic clostridium pasteurianum
  • Method for preparing hydrogen by fermentation through using special anaerobic clostridium pasteurianum

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] Example 1: Determine the basal medium for expanded culture of strains

[0023] The medium composition is: peptone 10g / L, beef extract 2.4g / L, yeast extract 5.0g / L, Na 2 HPO 4 4g / L, L-cysteine ​​0.2 g / L, L-cysteine ​​hydrochloride monohydrate 0.5 g / L, horse serum 40-60 mL / L, distilled water 1000 mL. In addition, 1 mL / L of resazurin indicator (0.2%) was added. Adjust pH=6.5, put 250mL saline bottle (with bottle stopper, can be sealed) per 100mL culture medium, heat the liquid in the bottle in a water bath, and blow nitrogen gas from the bottle mouth at the same time, until the liquid phase becomes colorless (the bottle is anaerobic environment), close the bottle cap. High temperature sterilization at 120°C for 25min, drying in a 60°C oven, inoculation of the strain Clostridium pasteurianum into a bottle containing a medium (aseptic anaerobic operation), and static culture at 37°C for 24-48h.

[0024] During the culturing process, the absorbance value of the sample...

Embodiment 2

[0027] Example 2: Selection and Determination of Substrate Concentration Configuration of Bacteria Fermentation Substrate

[0028] The composition of the fermentation medium is: peptone 10g / L, beef extract 2.4g / L, yeast extract 5.0g / L, Na 2 HPO 4 4g / L, L-cysteine ​​0.2g / L, L-cysteine ​​hydrochloride monohydrate 0.5g / L, horse serum 40-60 mL / L, resazurin (0.2%) 1mL / L, glucose 5~20g / L; adjust the initial pH=5.5~6.5; in the process of preparing the medium, use heating and boiling to expel the liquid phase dissolved oxygen and use nitrogen to blow off the gas phase air in the bottle to ensure strict anaerobicity at 115 ℃ high temperature sterilization for 25min; under aseptic anaerobic operation, the previously cultivated Clostridium pasteurianum OD 600 The inoculum high-concentration bacterial liquid and medium with a value of 1.402 were inoculated into the medium in the 200ml fermentation flask according to the inoculum amount in Table 1, and cultured in an anaerobic box a...

Embodiment 3

[0034] Example 3: Optimal fermentation pH determination

[0035] The effect of initial pH value on the total gas production and cell growth of C. pasteuri is shown in Tables 4 and 5. Under the condition of glucose concentration of 10 g / L, the total gas production of Clostridium pastoris fluctuated with the change of initial pH. When the initial pH is in the range of 5.0~6.0, the total gas production and cell dry weight increase with the increase of the initial pH. When the initial pH is 6.0, the total gas production reaches a maximum of 480 mL / L, and the cell dry weight reaches a maximum of 0.72 g. / L; when the initial pH continued to increase, both the total gas production and the dry cell weight decreased with the increase of the initial pH. This indicates that the initial pH of 6.0 is the optimal growth condition for Clostridium pastoris under the condition of 10g / L glucose concentration, and the value below or above this value will inhibit Clostridium pastoris.

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
concentrationaaaaaaaaaa
Login to View More

Abstract

The invention provides a method for producing hydrogen by fermenting obligate anaerobic Clostridium pasteuriani. Under sterile anaerobic conditions, using anaerobic bacteria, peptone, beef extract, yeast extract, Na2HPO4, L-cysteine A mixture of amino acid, L-cysteine ​​hydrochloride monohydrate, resazurin indicator and distilled water is used as the medium, glucose is used as the fermentation substrate, and hydrogen production is carried out by batch fermentation. The obligate The anaerobic bacteria was Clostridium pasteuriani JCM1408T. The composition of the medium is: peptone 10.0-15.0g, beef extract 2.0-3.0g, yeast extract 5.0-10.0g, Na2HPO4 3.0-4.0g, L-cysteine ​​0.2-0.5g, L-cysteine Hydrochloride monohydrate 0.2-0.5g, 0.2%wt resazurin indicator 1mL, horse serum 40-60ml and distilled water 1L. Hydrogen can be produced continuously.

Description

technical field [0001] The invention relates to a method for producing hydrogen by utilizing microbial anaerobic fermentation, in particular a method for producing hydrogen by utilizing highly efficient anaerobic bacteria. Background technique [0002] As a new generation of green energy, hydrogen is clean, efficient, and renewable. If it can be produced industrially, it will definitely be able to replace fossil energy such as oil and natural gas in the future and reduce the pressure on the environment. [0003] In addition, hydrogen energy is not a primary energy source and needs to be produced from hydrogen-containing compounds. At present, more than 90% of the hydrogen in the world comes from fossil combustion, and the rest is hydrogen production from water electrolysis. No matter what kind of hydrogen production method will directly or indirectly consume a large amount of fossil energy. The reserves of fossil fuels are limited and tend to be exhausted. At the same time...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): C12P3/00C12R1/145
Inventor 钱春香陈璐圆王瑞兴
Owner SOUTHEAST UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com