Energy-saving type culturing method of chaliotis diversicolor
A breeding method and technology for nine-hole abalone, which is applied in the field of energy-saving nine-hole abalone breeding, can solve problems such as adverse effects on body immunity, drug resistance and drug residues, health hazards in aquaculture, and prevent the bacterial infection of nine-hole abalone. Diseases, improve the survival rate of breeding, and improve the effect of immunity
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[0030] (1) Preparation of the Bdellovibrio Bdeloidoplasma Bacterial Liquid Used for the Growth of Haliotis diversicoloraquatilis
[0031] Inoculate 0.5mL10 in an Erlenmeyer flask containing 100mL nutrient broth liquid medium (peptone 10g, beef extract powder 3g, sodium chloride 5g, pH7.4±0.2).7 cfu / mL Aeromonas hydrohila (Aeromonas hydrohila, strain number: GIM1.172, from the Guangdong Provincial Microbial Culture Collection Center), 200rpm, 35°C shaker culture for 12 hours, the culture solution was centrifuged at 4°C, 5000rpm for 15min , discard the supernatant. Use 5mL normal saline to suspend and precipitate the bacteria, and then add it to a liquid medium containing 100mL DNB (0.8g nutrient broth, 0.5g casein acid hydrolyzate, 0.1g yeast extract, dissolve in 1000mL distilled water, the pH value is 7.2~7.6) into the Erlenmeyer flask, add 1mL containing 10 3 Bdellovibrio BDFM05 (CCTCCM209172) in pfu / mL. Incubate on a constant temperature shaker at 250 rpm at 28°C for 36 ho...
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