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Preparation method of bacteria-bran composite granular material used for adsorbing metal ion

A technology of composite particles and metal ions, which is used in alkali metal compounds, chemical instruments and methods, adsorption water/sewage treatment, etc., to achieve the effects of mild preparation conditions, easy preservation and large specific surface area.

Inactive Publication Date: 2011-06-01
FUJIAN NORMAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Bran has been used as an adsorption material for metal ions, but the research on the adsorption of metal ions in groundwater by combining bran and microorganisms to prepare granular materials has not been reported yet.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0014] 1. Preparation of composite bacteria liquid

[0015] Raw materials: Pseudomonas sp. and Acinetobacter sp. were purchased from China Industrial Microorganism Culture Collection and Management Center; wheat bran was purchased from Jianping Village, Minhou County, Fuzhou, Fujian.

[0016] Prepare modified JFMII medium (1000mL): NH 4 NO 3 1.0g, NaNO 3 1.0g, K 2 HPO 4 1.0g, MgSO 4 ·7H 2 O 1.0g, CaCl 2 0.4g, 4.0g citric acid, 8.0g trisodium citrate, 0.026g ferric ammonium citrate, add water to dissolve, adjust the pH to 7.2, and sterilize at 121°C for 20min.

[0017] (1) Transfer 1 ring of slant cultures with Pseudomonas and Acinetobacter to seed bottles respectively, and cultivate them at 26°C and 130r / min for 24 hours to obtain seed liquids in the logarithmic growth phase, which are then inoculated into Expanded culture in 5 liter culture tank. After culturing for 40 hours, concentrate the expanded cultured Pseudomonas and Acinetobacter bacterial liquids to the bac...

Embodiment 2

[0022] 1. Preparation of composite bacteria liquid

[0023] Raw material: with embodiment 1.

[0024] Prepare modified JFM II medium (1000mL): NH 4 NO 3 1.0g, NaNO 3 1.0g, K 2 HPO 4 1.0g, MgSO 4 ·7H 2 O 1.0g, CaCl 2 0.4g, 4.0g citric acid, 8.0g trisodium citrate, 0.026g ferric ammonium citrate, add water to dissolve, adjust the pH to 7.2, and sterilize at 121°C for 20min.

[0025] (1) Transfer 1 ring of Pseudomonas and Acinetobacter slant cultures to seed bottles, and cultivate them at 26°C and 130r / min for 24 hours to obtain seed liquids in the logarithmic growth phase, and then inoculate the seed liquids at 5 Expanded culture in liter culture tanks. After culturing for 40 hours, concentrate the expanded cultured Pseudomonas and Acinetobacter bacterial liquids to the bacterial concentration OD 600nm About 2.0, the volume (L) ratio of Pseudomonas bacterium liquid and Acinetobacter bacterium liquid is compared 1: 3 to prepare compound bacterial bacterium liquid.

[0...

Embodiment 3

[0030] 1. Preparation of composite bacteria liquid

[0031] Raw material: with embodiment 1.

[0032] Prepare modified JFM II medium (1000mL): NH 4 NO 3 1.0g, NaNO 3 1.0g, K 2 HPO 4 1.0g, MgSO 4 ·7H 2 O 1.0g, CaCl 2 0.4g, 4.0g citric acid, 8.0g trisodium citrate, 0.026g ferric ammonium citrate, add water to dissolve, adjust the pH to 7.2, and sterilize at 121°C for 20min.

[0033] (1) Transfer 1 ring of Pseudomonas and Acinetobacter slant cultures to seed bottles, and cultivate them at 26°C and 130r / min for 24 hours to obtain seed liquids in the logarithmic growth phase, and then inoculate the seed liquids at 5 Expanded culture in liter culture tanks. After cultivating for 40 hours, concentrate the Pseudomonas and Acinetobacter cultured solution to the bacterial concentration OD600nm About 2.0, the volume (L) ratio of Pseudomonas bacterium liquid and Acinetobacter bacterium liquid is compared 1: 3 to prepare compound bacterial bacterium liquid.

[0034] 2. Preparatio...

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Abstract

The invention relates to a method for preparing a metal ion adsorbing material in a water body, in particular for preparing bacteria liquid of composite bacteria by utilizing an improved JFM II culture medium and then preparing a bacteria-bran composite granular material. The preparation method is characterized in that (1) the improved JFM II culture medium comprises the following components of 0.5g of NH4NO3, 0.5g of NaNO3, 0.5g of K2HPO4, 0.5g of MgSO4.7H2O, 0.2g of CaCl2, 2g of citric acid, 8g of trisodium citrate and 0.013g of ammonium ferric citrate; and (2) bran is sterilized at high temperature; the bacteria liquid of the composite bacteria is added to be primarily and uniformly mixed; then carboxymethyl cellulose is added to be stirred; pelletizing is carried out in a pelletizer; and a mixture is naturally dried to prepare the bacteria-bran composite granular material. In the invention, the bran with favorable adsorbing effect and rich nutrient components is utilized as a supplementary material of the composite granular material, has the advantages of large specific surface area, favorable water retention and moisture retention and is easily stored; because the CMC (Carboxymethyl Cellulose ) is adopted, no biohazard or secondary pollution is generated; and the prepared granular material has favorable ferro-manganese removing capacity, is used for biological treatment on high concentration ferrimanganic ions in ground water in water treatment filtering and comes up to the corresponding emission standard.

Description

technical field [0001] The invention relates to a preparation method of a metal ion adsorption material in a water body, in particular to a preparation method of compounding bacteria and bran to prepare granular materials. The prepared pellets are mainly used in the treatment of high-concentration iron-manganese ions in groundwater to reduce the concentration of iron-manganese ions in groundwater and meet corresponding discharge standards. Background technique [0002] With the application and development of microbial technology, more and more functional bacteria have been discovered and utilized by people. Bacteria isolated and purified from a specific area have a special ability. For example, microorganisms living in a certain mining area often have high resistance to the metals with high content in this mining area, and can accumulate through a certain mechanism or precipitate through chemical action. special metal. In addition, in nature, microorganisms restrict each o...

Claims

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Application Information

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IPC IPC(8): B01J20/24B01J20/32B01J20/28C02F9/14C02F1/28C02F1/64
Inventor 李敏陈丽芳许旭萍陈登龙游亚平
Owner FUJIAN NORMAL UNIV
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