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Cell microcapsule for treating parkinsonism and composite cytoskeleton

A Parkinson's disease and microcapsule technology, applied in the field of cell microcapsules and composite cell scaffolds for the treatment of Parkinson's disease, can solve the problem of not seeing, prolonging the survival period of transplanted cells, and not seeing immune rejection of cytochrome epithelial cells, etc. To achieve the effect of prolonging the survival period, improving the success rate and cell survival rate, and continuously secreting dopamine

Active Publication Date: 2012-10-24
JIANGSU YONGNIAN LASER FORMING TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] At present, there is no report of cytochrome epithelial cells made into artificial cells to overcome immune rejection, and there is no report of combining with neurotrophic factors to prolong the survival of transplanted cells

Method used

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  • Cell microcapsule for treating parkinsonism and composite cytoskeleton
  • Cell microcapsule for treating parkinsonism and composite cytoskeleton

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0039] Example 1. Subculture of retinal pigment epithelial cells.

[0040] Primary retinal pigment epithelial cells were purchased from ScienCell, USA

[0041] The culture medium used is the EpiCM medium (EpiCM, Catalog Number is 4101) of U.S. ScienCell Company, and this medium component is that every 500ml medium contains 10ml fetal bovine serum FBS (Catalog No.0010), 5ml epithelial cell nutrient (Catalog No.4152) and 5ml penicillin streptomycin antibody (P / S, Catalog No.0503).

[0042] Cells were subcultured using 0.25% Trypsine as a digestion solution. The purchased primary cells are packed in 75cm2 culture flasks, placed at 37°C, 5% CO 2 Cultured in an incubator, the culture medium was changed every 3 days until the cells were confluent and passaged.

[0043] When the cells divide and proliferate to confluence, they should be subcultured in time, absorb the culture medium, wash with PBS buffer 3 times, digest with 0.25% trypsin for 3-5 minutes, observe under the inverte...

Embodiment 2

[0044] Example 2: Preparation of gelatin microspheres containing neurotrophic factors by single coacervation method

[0045] Prepare 5 ml of gelatin solution with a mass fraction of 20%, and sterilize it in an oven at 80° C. for 2 to 3 times, each time for 4 to 5 hours. Add 100ug neurotrophic factor (ScienCell, NGS, Catalog No.1562) under sterile conditions, add acetone dropwise at pH 3.8 to coagulate the gelatin solution, and then add glutaraldehyde to cross-link to obtain a smooth surface with a diameter of 200-400nm gelatin microspheres.

Embodiment 3

[0046] Embodiment 3. making sodium alginate-polylysine-sodium alginate cell microcapsules

[0047] Step 1. get the 75cm made in embodiment 1 2 Culture flasks grow close to confluent 3rd to 5th passage retinal pigment epithelial cells at a cell concentration of 8×10 6 ~10×10 6 / L

[0048] Step 2. Extract the cells, suck off the culture medium, add 0.25% trypsin to digest for 3-5 minutes, add culture medium (EpiCM, CatalogNumber is 4101) to stop, blow and mix well, centrifuge and discard the supernatant to obtain RPE .

[0049] Step 3, mix RPE with 1.5% sodium alginate to make a density of 1×10 6 / ml of cell suspension. Drop the cell suspension into gently agitated 1.1% CaCl with a No. 4 needle 2 , allowing it to form gel beads.

[0050] Step 4, the neurotrophic factor microspheres prepared in Example 2 are mixed with 0.05% polylysine in equal volumes,

[0051] Step 5. The gel beads are reacted sequentially with the mixture in step 4 and sodium alginate, and finally the ...

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Abstract

The invention relates to a cell microcapsule for treating parkinsonism and a composite cytoskeleton, relating to the field of transplantation treatment of the parkinsonism. The cell microcapsule contains cells which are epithelial cells of retinochrome; and a microcapsule shell is internally mixed with neurotrophic factors. The invention provides a feasible method for treating the human parkinsonism by using the epithelial cells of heterogenous retinochrome, which treats the pathological brain tissues of a patient in a transplanting way together by adding the neurotrophic factors to the microcapsule shell. The invention not only directly plays the neuron repair function by solving the problems of clinical application, but also prolongs the survival time of transplanted cells with the epithelial cells of the retinochrome through synergistic action, thereby providing an ideal way for relieving the symptoms of the parkinsonism and repairing pathological cells.

Description

technical field [0001] The invention relates to the field of Parkinson's disease transplantation therapy, in particular to a cell microcapsule and a composite cell scaffold for treating Parkinson's disease. Background technique [0002] Parkinson's disease (PD for short) is a sudden and slowly progressive degenerative disease of the central nervous system. Its pathological feature is mainly the selective degeneration and death of dopaminergic neurons in the substantia nigra of the midbrain. With loss, muscle rigidity, resting tremor and postural instability, also known as parkinsonis, is the most common neurodegenerative disorder. [0003] At present, there is no way to prevent or completely cure Parkinson's disease. The principle of treatment is the combination of medicine and surgery. However, drug therapy and surgical methods for Parkinson's disease can only improve some symptoms, and the long-term efficacy is not optimistic. Since Parkinson's disease is mainly caused b...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K9/50A61K38/18A61P25/16A61L27/38A61L27/26A61L27/20A61L27/18A61L27/54A61L27/56A61K35/44
Inventor 张定军郭戈唐果林颜旭涛戴丽娜
Owner JIANGSU YONGNIAN LASER FORMING TECH
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