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Yeast grease and preparation method and application thereof

A technology of oil and yeast, which is applied in the fields of bioengineering, food and medicine, and can solve problems such as production technology staying in the laboratory stage

Inactive Publication Date: 2011-06-22
BINZHOU UNIV +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although oleaginous yeast is a good source of new oils and fatty acids, its production technology is only at the laboratory stage, and a lot of research work is still needed to achieve the purpose of industrial production and application

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0095] Extraction of Brewer's Yeast Oil

[0096] 2000g of brewer's yeast sludge was repeatedly washed with water for 3 times by suction filtration, and dried at 60°C at low temperature to obtain 178g of dry yeast. Crush, weigh 100g of yeast powder, add 500ml of n-hexane, reflux for extraction for 1h, filter with suction, collect the filtrate, add 400ml of n-hexane to the filter residue to repeat the extraction once, and filter with suction. The two filtrates were combined, and n-hexane was recovered by distillation to obtain yeast oil 6.37, wherein the triterpene content (calculated as squalene) was 2.12 g. Redissolve the oil with 200ml of ether, filter with suction, collect the filtrate, distill and reclaim the ether, and continue heating in a water bath to evaporate the ether to obtain 5.72g of refined oil, wherein the triterpene content (calculated as squalene) is 2.01g. g.

Embodiment 2

[0098] Oil Extraction of Rhodotorula viscosus

[0099] (1) Pretreatment of yeast raw materials

[0100] The fermented Rhodotorula viscosus fermentation broth is pressed into a three-legged centrifuge or a plate-and-frame filter press, rinsed with water while centrifuging, and dried; or washed with a plate-and-frame filter press to remove excess water, and the bacteria are collected The body is dried by a vibrating fluidized bed dryer or a flat panel dryer so that the water content is about 8%-10% (w / w), and then enters the steaming and frying process of the yeast raw material;

[0101] (2) Steam and stir-fry the fungus

[0102] Put the bacterial material treated in step 1 into the steaming wok, the charging coefficient is 0.8-0.9, spray water and direct steam into the bacterial material, make the bacterial material fully contact with water and moisten evenly by stirring, the stirring speed is 25- 40r / min, turn off the exhaust, heat the bacterial material to a temperature of 95...

Embodiment 3

[0113] Refining of Rhodotorula viscosum oil by supercritical CO 2 Extraction

[0114] 700g of Rhodotorula viscosus grease obtained in Example 2, wherein 20mL of absolute ethanol was added and stirred evenly, and the alcohol-oil mixture was added to the extraction kettle at one time, to CO 2 Cool the storage tank to 4°C, heat the extraction kettle and the desorption kettle respectively, when the temperature reaches 40°C and 32°C respectively, and the pressure of the extraction kettle and the desorption kettle reaches 35MPa and 10MPa respectively, keep the static pressure for 5-25min, turn off the CO 2 Cylinder, start circulation extraction, adjust CO 2 The flow rate was 20L / h, constant temperature and pressure extraction for 1.5h, and refined oil was obtained from the analytical kettle; the yield was 96.27%; the oil was subjected to supercritical CO 2 After extraction and refining, the content of unsaturated fatty acid increases by 10-25%.

[0115] After testing, the oil con...

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PUM

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Abstract

The invention relates to the technical field of biological engineering and the fields of food and medicaments, particularly provides a method for extracting refined yeast grease from yeast, particularly waste saccharomyces cerevisiae and oil production yeast, and further provides soft capsules prepared by compounding the yeast grease, vegetable grease rich in necessary fatty acid for a human bodyand anti-oxidation grease and a preparation method thereof. The obtained yeast grease composite soft capsules have the effects of reducing serum cholesterol and reducing blood fat; the method for producing the yeast grease by using the waste yeast has powerful results of improving the additional value of the waste yeast and generating higher economic benefit; and meanwhile, by comprehensive utilization of the waste yeast, the problem that wastes pollutes environment is solved, and the requirement of circular economy development is met.

Description

technical field [0001] The present invention relates to the technical field of bioengineering and the field of food and medicine, in particular to a kind of oil extracted by using yeast as a raw material, and a method for preparing soft capsules by using the oil, in particular to a soft capsule prepared by using oil extracted from beer waste yeast and its preparation method. Background technique [0002] Brewer's yeast is a certain species of Saccharomyces cevevisiae, which contains a variety of biologically active substances, mainly about 50% of protein, 6-8% of nucleic acid, about 10% of polysaccharide, about 6-9% of Fat, also rich in B vitamins, vitamin D 2 In addition, it also contains a variety of coenzymes and other physiologically active substances with high economic value. [0003] Oleaginous yeast is a yeast species with a cell oil accumulation of more than 20%, and some species such as Pulmonary yeast and oleaginous yeast have an oil content of more than 60%. Th...

Claims

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Application Information

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IPC IPC(8): A23D7/00A23D7/02A23D7/04C11B1/06C11B1/10C11B3/12
CPCY02E50/343Y02P20/54
Inventor 王宝琴徐泽平
Owner BINZHOU UNIV
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