Rape BnPABP 5 gene and application of promoter thereof

A promoter and gene technology, applied in the field of plant genetic engineering and biology, can solve the problems of abnormal anther development and male sterility in plants
CN102115751AInactive Publication Date: 2011-07-06INST OF OIL CROPS RES CHINESE ACAD OF AGRI SCI
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Patent Information

Authority / Receiving Office
CN · China
Current Assignee / Owner
INST OF OIL CROPS RES CHINESE ACAD OF AGRI SCI
Publication Date
2011-07-06
Estimated Expiration
Not applicable · inactive patent

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Abstract

The invention discloses a rape BnPABP 5 gene and the application of a promoter thereof. The sequence of a segregation gene is nucleotide sequence indicated by SEQ ID No. 1, SEQ ID No.2. The promoter comprises a recombinant vector. The invention also discloses the application of the rape BnPABP 5 gene the promoter thereof in controlling pollen fertility and anther. The gene and the promoter thereof are predominantly expressed in the rape anther. The invention further relates to the isolation, cloning, analysis of expression and application of gene BnPABP5 and the promoter thereof for controlling microspore development and the anther fertility. The BnPABP 5 gene has the function of controlling the anther development of rape as well as the pollen fertility. The expression of the BnPABP 5 gene can be weakened through the technique of gene engineering so that the pollen fertility can be controlled and male sterile line materials as well as restorer line materials can be created, therefore, the rape BnPABP 5 gene can be applied to the development and research and the quality improvement of rape andro gamete. The promoter of the BnPABP 5 gene is only predominantly expressed in the anther, and the promoter with tissue preference has higher potential of edible safety on transgenic oil crops, thus having certain development and application value.
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Description

technical field

[0001] The invention relates to the fields of plant genetic engineering and biotechnology. It specifically relates to a gene related to pollen development, a promoter controlling the high-efficiency expression of the gene in pollen or anther, and a preparation method of the gene and the promoter. The present invention also relates to a vector containing the gene and its promoter or its homologous nucleotide sequence and relates to the application of the gene or its promoter in rapeseed genetic engineering. Background technique

[0002] Most eukaryotic mature mRNA molecules have a poly(A) tail, which is related to the stability of mRNA and the regulation of translation initiation. Poly(A) binding protein (poly(A)binding protein, PABP) widely exists in different types of eukaryotic cells, and has a strong affinity with 25bp or longer poly(A) (Sachs A B, Davis R W and Kornberg R.D.A single domain of yeastpoly(A)-binding protein is necessary and sufficient for ...

Claims

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