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New hydroxamate siderophore and application thereof in prevention and control of disease

A siderophore, hydroxamic acid technology, applied in the preparation of hydroxamic acid siderophore, the application field of cucumber wilt, to achieve the effect of easy degradation, good application prospect and low toxicity

Inactive Publication Date: 2011-10-12
ZHEJIANG ACADEMY OF AGRICULTURE SCIENCES
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The research on its purification and structural analysis started earlier, but there are few reports in China. So far, more than 500 kinds of siderophiles with different chemical structures have been found.

Method used

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  • New hydroxamate siderophore and application thereof in prevention and control of disease
  • New hydroxamate siderophore and application thereof in prevention and control of disease
  • New hydroxamate siderophore and application thereof in prevention and control of disease

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] 1. Experiment with all instruments and equipment

[0032] UV: Jasco V550 ultraviolet spectrophotometer

[0033] IR: JascoFT-IR4100 infrared spectrometer

[0034] NMR: BRUCKER AVANCE DMX500 Superconducting NMR

[0035] Mass spectrometry: ESI-MS: HP5989B mass spectrometer

[0036] APEX III 7.0 Fourier Transform Ion Cyclotron Resonance Mass Spectrometer

[0037] Satorius BSA124S Electronic Balance Beijing Sartorius Balance Co., Ltd.

[0038] Low temperature high speed centrifuge BECKMAN

[0039] HPLC Elite

[0040] Solvents: all commercially available AR grade Tianjin Yongda Chemical Reagent Co., Ltd.

[0041] Column chromatography: LS610 macroporous adsorption resin Zhejiang Academy of Agricultural Sciences

[0042] ODS Reversed Phase Silica Gel Beijing Huideyi Technology Co., Ltd.

[0043] Reversed phase high performance thin layer chromatography plate Merk company

[0044] Chromogen: UV lamp, iodine cylinder, phosphomolybdic acid chromogen

[0045] The glassware...

Embodiment 2

[0057] Example 2: Structural identification and data assignment of siderophilic compounds

[0058] The compound is light yellow solid, Mp: 239-240°C (decomposition). Difficult to dissolve in methanol, easily soluble in water, and the detection of CAS detection solution shows that there is a color change compared with the control CAS detection solution, the sample is spread on the RP-18 reversed-phase thin-layer chromatography plate, and the reverse-phase thin-layer chromatography iodine cylinder The color is yellowish brown. Description may be hydroxymates type siderophore.

[0059] Molecular formula: C20H23N4O5

[0060] 1 The HNMR spectrum gives 9 groups of peaks whose integration ratio (from downfield to upfield) is 4:2:4:2:1:1:1:2:2.

[0061] a δ3.11 and 3.27 are both dd peaks, corresponding to 2 protons respectively, and both have the same coupling constant (J=14.5), and at the same time 1 H- 1 The H COZY spectrum shows that the two are correlated, so the δ3.11 and...

Embodiment 3

[0093] Embodiment 3: the anti-disease effect test of steodin to cucumber wilt

[0094] The structure of the compound of steodin is (as follows)

[0095]

[0096] The cucumber seeds used in this experiment were not treated with any seed coating agent. After disinfection, they were sown in plastic pots with fertile vegetable garden soil substrates sterilized twice by high pressure. The seedling pots were placed in the Institute of Plant Protection and Microbiology, Zhejiang Academy of Agricultural Sciences. In the greenhouse, after the cotyledons were fully expanded, induction inoculation and challenge inoculation were carried out at the same time.

[0097] The test of disease resistance effect adopts the greenhouse potting method: the sample compound is dissolved in distilled water and prepared into a 50 mg / L aqueous solution, the blank control uses clear water and the positive control uses 50 mg / L carbendazim. The root irrigation method was used to induce inoculation, and ...

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Abstract

The invention relates to a new siderophore and application thereof in prevention and control of cucumber fusarium wilt. The hydroxamate siderophore is obtained from a plant growth promoting bacteria pseudomonas putida HZ-2 strain by extraction with an organic solvent, conventional column chromatography and high performance liquid chromatography separation. The hydroxamate siderophore has certain prevention and control effect on agricultural diseases, so the hydroxamate siderophore can be used for preparing novel biological pesticides.

Description

technical field [0001] The invention relates to a new siderophile, in particular to a preparation method of a hydroxamic acid siderophile and its application in cucumber wilt. Background technique [0002] The use of beneficial bacteria to prevent and control diseases and insect pests has become a hot topic in current research. Up to now, many strains have been successfully developed into products and promoted. Their importance in pest control comes from some of their inherent properties. Pseudomonas spp. is a class of biocontrol bacteria that has been extensively studied. This type of bacteria can produce special substance siderophiles, and the secretion of siderophiles is one of the main mechanisms for controlling fungal diseases. A hot topic of research by workers. Siderophiles are a class of small molecular weight (500-1000 Da) that can specifically chelate Fe 3+ Most bacteria and fungi can synthesize and secrete one or several kinds of siderophiles through the non-ri...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07C259/06A01N37/28A01P3/00A01P1/00
Inventor 梁建根陶荣祥施跃峰郝中娜王连平陈建忠陈识峰竺利红吴吉安
Owner ZHEJIANG ACADEMY OF AGRICULTURE SCIENCES