Lyophilization process of lactobacillus plantarum

A technology of freeze-drying of Lactobacillus plantarum, applied in the field of freeze-drying process of Lactobacillus plantarum, to achieve the effect of improving the survival rate of freeze-drying

Inactive Publication Date: 2011-10-19
BEIJING ACADEMY OF AGRICULTURE & FORESTRY SCIENCES
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, in the existing freeze-drying process of Lactobacillus plantarum, for factors such as protective agent pH, prefreezing time, and equilibrium time, it is rare to see that there is special research on optimizing this aspect, but the test of the present invention finds that these factors It has a very important impact on the freeze-drying survival rate of Lactobacillus plantarum

Method used

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  • Lyophilization process of lactobacillus plantarum
  • Lyophilization process of lactobacillus plantarum
  • Lyophilization process of lactobacillus plantarum

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] Embodiment 1 Protective agent pH comparison test.

[0035] 1.1 Materials

[0036] Lactobacillus plantarum, preserved by the Laboratory of Animal Nutrition and Breeding, Institute of Animal Husbandry and Veterinary Medicine, Beijing Academy of Agriculture and Forestry Sciences, was isolated from the cecal mucosa of 60-day-old weaned piglets, and was identified as Lactobacillus plantarum by the China Industrial Microbiology Collection Management Center .

[0037] Lyoprotectant: 14% skimmed milk powder + 2% glycerin + 4% sucrose + 0.15% sodium glutamate + 10% dextrin.

[0038] Counting medium (MRS medium): peptone 10.0g, beef extract 10.0g, yeast powder 5.0g, glucose 20.0g, anhydrous sodium acetate 5.0g, diammonium citrate 2.0g, Tween-801.0ml, dihydrogen phosphate Potassium 2.0g, magnesium sulfate 0.58g, manganese sulfate 0.19g, agar 15g, distilled water 1000ml, pH 6.5, sterilized at 121°C for 20min.

[0039] 1.2 Freeze-drying steps

[0040] The basic parameters of fre...

Embodiment 2

[0048] Embodiment 2. Effect of bacteria slime and protective agent equilibrium time on freeze-drying survival rate

[0049]Centrifuge the sludge and add it to the protective agent. Set 5 treatments. The equilibrium time between the sludge and the protective agent in the 5 treatments is 10, 20, 30, 40, and 50 minutes. Other parameters are the same as in Section 1.2, and then freeze-dried , measure the number of viable bacteria in the freeze-dried powder, and calculate the freeze-dried survival rate, the results are shown in Table 2.

[0050] Table 2 Effect of protective agent and bacteria slime equilibrium time on freeze-dried survival rate of Lactobacillus plantarum

[0051]

[0052] It can be seen from the results that the freeze-dried survival rate of Lactobacillus plantarum gradually increased with the prolongation of the balance time of the protective agent and the bacteria slime. When the balance time was 30 minutes, the freeze-dried survival rate of the bacteria reach...

Embodiment 3

[0053] Embodiment 3. The impact of pre-freezing time on freeze-dried survival rate

[0054] Set up 6 treatments, mix the bacteria slime and protective agent, and take the pre-freezing time as 0.5, 1.0, 1.5, 2.0, 2.5, 3.0 hours respectively, other parameters are the same as section 1.2, measure the number of viable bacteria in the freeze-dried powder, and calculate the freeze-dried survival rate. The results found that when the pre-freezing time was short, the freeze-dried survival rate of Lactobacillus plantarum showed an upward trend, and the freeze-dried survival rate of the strains was the best when the pre-freezing time was 2.0 hours, and then as time went on, the freeze-dried survival rate of the strains increased. start to fall. Considering time efficiency and freeze-drying survival rate comprehensively, 1.5-2.5 hours is a relatively preferable parameter, as shown in Table 3.

[0055] Table 3 The influence of pre-freezing time on the freeze-dried survival rate of Lacto...

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Abstract

The invention relates to a lyophilization process of lactobacillus plantarum, and belongs to a microbial agent preparation process. In the lyophilization process of lactobacillus plantarum, the process parameters are that the pH of a lyophilization protective agent is 7.0-7.5, the balancing time of bacterium mire and the protective agent is 20-40minutes, pre-lyophilization is carried out for 2.0-2.5h under the condition of minus 80 DEG C, a phosphate buffer is adopted to prepare the protective agent, lyophilization livability can be up to over 90%, and the viable count can be up to over 4.0*1010cfu/g. Compared with the general method, the method disclosed by the invention has the advantages that the livability of the lactobacillus plantarum is improved remarkably.

Description

technical field [0001] The invention relates to a preparation process of microbial preparations, in particular to a freeze-drying process of live bacterial preparations composed of plantaractobacillus and directly fed to animals. Background technique [0002] Lactic acid bacteria are normal intestinal flora of humans and animals. Numerous studies have proved that the bacteria can inhibit the growth and reproduction of pathogenic bacteria (such as Salmonella, Shigella, Helicobacter, etc.), promote the body's digestion and absorption of nutrients, and improve Immunity and other aspects have important biological functions. In recent years, with people's gradual understanding of many disadvantages of antibiotics, and countries around the world have successively banned the use of antibiotics in feed, lactic acid bacteria probiotic preparations, as a new type of green feed additive, are attracting more and more attention. It also has good development prospects. [0003] However,...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12N1/04C12R1/25
Inventor 季海峰张董燕刘辉王四新王雅民王晶单达聪
Owner BEIJING ACADEMY OF AGRICULTURE & FORESTRY SCIENCES
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