Nucleic acid gold-labeled rapid detection method and kit for pathogen

Abstract

The invention discloses a nucleic acid gold-labeled rapid detection method and kit for a pathogen, belonging to the technical field of medical biotechnology, wherein the advantages of a colloidal gold-labeling technology and a nucleic acid PCR (Polymerase Chain Reaction) detection technology are combined; no instrument is relied except a PCR amplifier; the method and kit have an important significance to the large-scale screening and the blood product screening with the outbreak and prevalence of infectious diseases; in particular, the method and kit detect the early infection of an HBV virus, screen the potential HBV carriers in a blood donation source, ensure the safety of blood products, and realize a purpose of early, specifically, sensitively and economically detect the pathogen.

Description

technical field [0001] The invention relates to medical biotechnology, in particular to a nucleic acid gold standard rapid detection method for pathogens. Background technique [0002] Immunogold labeling technique (Immunogold labeling technique) is a solid-phase labeling immunoassay technique developed in the 1980s after the three major labeling techniques of fluorescein, radioisotope and enzyme. This technology mainly utilizes the characteristics of high electron density of gold particles. When these markers accumulate in a large amount at the corresponding ligands, red or pink spots visible to the naked eye are formed, so they are used in qualitative or semi-quantitative rapid immunoassay methods. middle. Rapid diagnostic test strips are a new type of in vitro diagnostic technology developed on the basis of monoclonal antibody technology, colloidal gold immunochromatography technology and new chromatographic materials since the 1990s. It has developed rapidly in recent y...

AI Technical Summary

Problems solved by technology

Although almost all blood donation sources have to go through HBV screening, since the current screening of HBV in my country is still based on the detection of pathogen antigens, for those in the "window period" ("window period" is after the virus infection During the period until the body produces specific antibodies, because both the virus content and the titer of the antigen in the body are very low, the detection is very difficult and often missed) patients, chronic carriers of occult infection, antigen or antibody titers in the serum The accuracy is lower than the current detection level and patients infected with mutant strains, but the current detection method may cause missed detection, which poses a great biological safety hazard

Real-time fluorescent quantitative PCR based on HBV nucleic acid detection can sensitively and specifically detect low-copy HBV in patients, but it requires expensive fluorescent quantitative PCR instruments and professionally trained inspectors to analyze the test results, which is not conducive to the majority of grassroots units, Hospitals, field workers, and large-scale time-critical testing and large-scale census

[0005] At present, there is no rapid detection method and kit for pathogens that have both the specificity and sensitivity of fluorescent quantitative PCR and the convenience of colloidal gold labeling detection.

Images