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Chitosan modified intelligent tissue engineering scaffold material and preparation method thereof

A technology of tissue engineering scaffold and chitosan, applied in animal cells, etc., can solve the problems of damaged cell function, poor biocompatibility, non-temperature sensitivity, etc., and achieve the goal of enhancing spreading and proliferation, avoiding damage, and good comprehensive performance Effect

Inactive Publication Date: 2011-11-23
TIANJIN POLYTECHNIC UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0008] According to the above analysis, the material modified by PNIPAAm can realize the non-damage desorption of cells through its temperature responsiveness, but due to its poor biocompatibility, it is not conducive to the adsorption and proliferation of cells on the surface of the material; the material modified by Cs , which enhances the biocompatibility of the material and is conducive to the spreading and proliferation of cells on its surface. However, due to the lack of temperature sensitivity, the cells cannot detach automatically. Using traditional enzymatic hydrolysis to detach cells will definitely damage cell functions.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] (1) Surface cleaning: Rinse the TCPS sample cut into 1cm×1cm with absolute ethanol to remove surface impurities, then rinse with distilled water several times, and dry it in vacuum at 60°C for later use.

[0031] (2) Temperature-sensitive carboxylation modification on the surface of TCPS: Dissolve 0.01 mol NIPAAm and 0.001 mol AAc in 13.6 mL DMSO, then add 0.32 g AQS as photosensitizer, and drop a few drops of isopropanol. Put the processed TCPS sample into the reaction solution, pass N 2 Seal for 15 minutes and irradiate for 4.5 hours with a high-pressure mercury lamp (λ=360nm, 200W). The distance between the high-pressure mercury lamp and the sample is 40cm, and the depth of the reaction solution is 1cm. Unreacted small molecules and unmodified polymers were then vacuum-dried for 1 day to obtain TCPS-NIPA-AAc on the thermosensitive carboxylated surface of TCPS.

[0032] (3) Saccharification modification on the surface of TCPS: Dissolve 60mgCs and 6mg Sulfo-NHS in 5mL...

Embodiment 2

[0034] (1) surface cleaning: with example 1 (1).

[0035] (2) Temperature-sensitive carboxylation modification on the surface of TCPS: Dissolve 0.01 mol NIPAAm and 0.005 mol AAc in 6.8 mL DMSO, then add 0.32 g AQS as photosensitizer, and drop a few drops of isopropanol. Put the processed TCPS sample into the reaction solution, pass N 2 Seal for 15 minutes, irradiate with a high-pressure mercury lamp (λ=360nm, 1000W) for 0.5h, the distance between the high-pressure mercury lamp and the sample is 40cm, and the depth of the reaction solution is 1cm. Unreacted small molecules and unmodified polymers were then vacuum-dried for 1 day to obtain TCPS-NIPA-AAc on the thermosensitive carboxylated surface of TCPS. (3) Saccharification modification on the surface of TCPS: Dissolve 10 mg Cs and 1 mg Sulfo-NHS in 2 mL 3% HCl solution, put the TCPS-NIPA-AAc sample into it, place it on a shaker at room temperature for 3 days, and add 20 mg of 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide hy...

Embodiment 3

[0037] (1) surface cleaning: with example 1 (1).

[0038] (2) Temperature-sensitive carboxylation modification on the surface of TCPS: Dissolve 0.01 mol NIPAAm and 0.005 mol AAc in 13.6 mL DMSO, then add 0.32 g AQS as photosensitizer, and drop a few drops of isopropanol. Put the processed TCPS sample into the reaction solution, pass N 2 Seal for 15 minutes and irradiate for 4.5 hours with a high-pressure mercury lamp (λ=360nm, 200W). The distance between the high-pressure mercury lamp and the sample is 40cm, and the depth of the reaction solution is 1cm. Unreacted small molecules and unmodified polymers were then vacuum-dried for 1 day to obtain TCPS-NIPA-AAc on the thermosensitive carboxylated surface of TCPS.

[0039] (3) Saccharification modification on the surface of TCPS: Dissolve 10mg Cs and 1mg Sulfo-NHS in 2mL 2% HCl solution, put the TCPS-NIPA-AAc sample into it, place it on a shaker at room temperature and shake for 3d, add to the reaction system every day 60 mg ED...

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Abstract

The invention discloses a chitosan modified intelligent tissue engineering scaffold material and a preparation method thereof. The invention belongs to the field of the functional macromolecular material and particularly relates to the intelligent tissue engineering scaffold material and the preparation method thereof. The temperature response range of the scaffold material is 31-33 DEG C. the preparation method of the scaffold material comprises the following steps: adopting the ultraviolet curing method to dissolve temperature sensitive substances N-isopropylacrylamide (NIPAAm) and acrylic acid (AAc) in dimethyl sulfoxide (DMSO) according to a certain ratio, using anthraquinone-2-sulfonic acid (AQS) as a photosensitizer to graft on the surface of a polystyrene cell culture plate, and then using N-hydroxysulfosuccinimide sodium salt (sulfo-NHS) and 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide (EDC.HCl) as an activator and a condensing agent to fix chitosan (Cs) with good biocompatibility on the surface of the polystyrene cell culture plate and prepare the chitosan modified intelligent tissue engineering scaffold material. The preparation method disclosed by the invention has a simple process, high maneuverability, low investment cost, a wide application range and safety and is easy to be popularized and used in industrial production.

Description

technical field [0001] The invention relates to an intelligent tissue engineering support material and technology, in particular to a temperature-sensitive support material for cell culture and desorption and a preparation method thereof. Background technique [0002] The damage, loss or failure of tissues and organs is one of the most frequent, harmful and costly problems in human health care. Every year, many patients suffer from tissue and organ damage or loss of function. It is a big problem that plagues the medical field. Currently, organ transplantation or artificial substitutes are mostly used in the treatment of tissue and organ damage. Research in this area is very limited, and the emergence of tissue engineering has opened up a new way to solve these problems. [0003] Tissue engineering is one of the most challenging cutting-edge scientific and technological fields in the field of medical life sciences in the past 20 years. It marks the advent of the era of repl...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08J7/18C08J7/12C08L25/06C12N5/07
Inventor 贺晓凌陈莉王雅晨
Owner TIANJIN POLYTECHNIC UNIV
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