Novel process for extracting superoxide dismutase SOD from animal blood cell liquid

A technology of superoxide and dismutase, which is applied in the new process field of extracting superoxide dismutase SOD, can solve the problems of undeveloped and utilized animal blood resources, waste of precious resources, and imperfect processing technology, etc., and achieve the reduction of consumption , reduce energy consumption, and compress volume

Inactive Publication Date: 2011-11-23
天津宝迪农业科技股份有限公司
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  • Summary
  • Abstract
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  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, due to the imperfect processing technology, the vast majority of animal blood resources in my country have not been developed and utilized, resulting in serious waste of precious resources and environmental pollution.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] (1) Collect 175L of fresh pig blood, add 7L of 10% sodium citrate for anticoagulation, and use a tubular centrifuge to continuously separate 70L of blood cells and 112L of plasma. Plasma is spray-dried into plasma protein powder, blood cells are pumped into the reaction tank, and 70L of deionized water is added at the same time and stirred for 60 minutes to break the blood cells to obtain 140L of lysed blood;

[0025] (2) With continuous stirring, add 4.06Kg of sodium chloride to the hemolysis, then adjust the pH value to 6.0 with 10% hydrochloric acid, heat to 62°C and keep it warm for 60min, quickly cool it to room temperature, and collect it by centrifugation in a three-legged centrifuge. Clear liquid obtains 100L of SOD crude extract;

[0026] (3) The crude extract is concentrated by ultrafiltration through a hollow fiber membrane with a molecular weight cut-off of 10K, and the reflux pressure is maintained at 0.08MPa to obtain 1L of SOD concentrated solution;

[0...

Embodiment 2

[0031] (1) Collect 50L of fresh pig blood, add 175g of sodium citrate for anticoagulant treatment, separate 20L of blood cells and 30.17L of plasma with a tubular centrifuge, pump the blood cells into the reaction tank, and add 40L of deionized water to stir hemolysis for 90min ;

[0032] (2) With continuous stirring, add 696g of sodium chloride to the hemolysate, adjust the pH to 7.0 with hydrochloric acid, heat to 70°C and keep it warm for 30min, quickly cool it to below 50°C, and collect 52L of SOD crude extract by centrifugation in a three-legged centrifuge ;

[0033] (3) The crude liquid is concentrated by ultrafiltration through a hollow fiber membrane with a molecular weight cut-off of 6K, and the reflux pressure is kept at 0.1 MPa, and the crude extract is concentrated to 1L;

[0034] (4) Add 0.5 L of 4°C pre-cooled acetone to the concentrated solution under stirring, continue stirring for 20 minutes, and collect the supernatant by centrifugation;

[0035] (5) Add 0....

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PUM

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Abstract

The invention discloses a novel process for extracting superoxide dismutase SOD from animal blood cell liquid. The process mainly comprises the following steps of: after carrying out anticoagulation treatment on animal fresh blood, centrifuging and separating blood cells; carrying out hemolysis, thermally denaturing, super-filtering and concentrating, and then carrying out fractional precipitation by acetone to obtain a rough product of the SOD; subjecting the rough product of the SOD to chromatographic purification, and then freezing and drying to obtain a competitive product of the SOD. In the process provided by the invention, chloroform ethanol is abandoned and transitional metal salt which is any one of copper salt, zinc salt and manganese salt in the process of thermally denaturing is not added; a ultra-filtering and concentrating process is further introduced on the base and the use amount of the acetone in the process of carrying out the fractional precipitation by acetone is extremely small. The novel process for extracting the SOD guarantees the activity of the traditional SOD to the maximum extent, has the advantages of simplicity in operation and low cost and is suitable for large-scale industrial production.

Description

Technical field: [0001] The invention relates to a process for extracting superoxide dismutase SOD, in particular to a new process for extracting superoxide dismutase SOD from animal blood cells. Background technique: [0002] Superoxide dismutase, referred to as SOD, is an important antioxidant enzyme in organisms and is widely distributed in various organisms. In the body, SOD can specifically remove excessive oxygen free radicals, effectively prevent the poisonous effect of active oxygen on organisms, and have obvious therapeutic effects on diseases caused by superoxide anion free radicals; in vitro, SOD has anti-aging , anti-tumor, anti-radiation, enhancing human immunity and other functions, are now widely used in medicine, food, health care products, cosmetics and other industries, the market prospect is very broad. [0003] Since the 1970s, it was first reported abroad that CuZn-SOD was purified by multi-stage precipitation method. So far, domestic and foreign biolo...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N9/08
Inventor 叶跃国臧盛王雅静卢进峰方俊生袁训安尹伦
Owner 天津宝迪农业科技股份有限公司
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