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High-efficiency formaldehyde degrading bacteria and use and using method thereof

A formaldehyde-degrading bacteria and high-efficiency technology, applied in chemical instruments and methods, biochemical equipment and methods, and microbial-based methods, can solve the problems of slow degradation rate, high biological toxicity, and difficult microbial degradation, and achieve fast degradation speed , strong tolerance, and simple fermentation and cultivation methods

Active Publication Date: 2012-11-21
CHENGDU INST OF BIOLOGY CHINESE ACAD OF S
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The purpose of the present invention is to provide a high-efficiency degrading bacterium capable of rapidly degrading high-concentration formaldehyde in view of the problems of high-concentration formaldehyde waste liquid produced in the production process of various industries and formaldehyde pollution accidents, such as high biological toxicity, difficult microbial degradation, and slow degradation rate. Its application in formaldehyde wastewater treatment and emergency treatment of formaldehyde pollution accidents

Method used

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  • High-efficiency formaldehyde degrading bacteria and use and using method thereof
  • High-efficiency formaldehyde degrading bacteria and use and using method thereof
  • High-efficiency formaldehyde degrading bacteria and use and using method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0011] Example 1: Screening and isolation of formaldehyde-degrading bacteria BZ-001H, domestication and treatment of formaldehyde wastewater

[0012] Collect activated sludge from the aerobic pool of Chengdu No. 1 Sewage Treatment Plant, inoculate 100ml of formaldehyde in selective medium (0.05%) according to 10% inoculum amount, carry out plate streak separation and purification, and screen to obtain formaldehyde that can use formaldehyde as a carbon source for growth. strain. The domestication of the strain adopts the directional intermittent domestication method of gradually increasing the formaldehyde concentration in the medium.

[0013] Synthetic Medium (MSB): Contains Na per 1L 2 HPO 4 2g, KH 2 PO 4 0.5g, MgSO 4 ·7H 2 O 0.3 g, (NH 4 ) 2 SO 4 0.5 g, trace element solution 5mL, formaldehyde 1g. Add 15 g / L agar powder to the solid medium.

[0014] After the sludge sample is enriched and cultured, a mixed microbial culture is obtained, which can grow with fo...

Embodiment 2

[0024] Example 2: BZ-001H different inoculum amounts Enhanced treatment system for high concentration formaldehyde waste liquid

[0025] After the BZ-001H strain was expanded and cultivated, the BZ-001H strain was inoculated according to the gradient (0.05‰, 0.1‰, 0.2‰, 0.3‰, 0.5‰, 0.7‰) of different inoculum amounts (based on dry cell weight of the strain) to the conventional An enhanced treatment system was constructed in the activated sludge treatment system (MLSS=2 000 ±100 mg / L), and 4356.7 mg / L of formaldehyde was added to domestic sewage to make a simulated waste liquid of sudden pollution accident, 30 ℃, 160 rmin -1 Cultivate on a shaking table, and take samples after 6 hours to measure the degradation of formaldehyde to determine the optimal inoculum size.

[0026]

[0027] The results in Table 3 show that the degradation of 4356.7 mg / L formaldehyde by BZ-001H, after 6 hours of degradation, the removal rate of formaldehyde in the waste liquid with the inoculation...

Embodiment 3

[0028] Example 3: Effects of pH on Formaldehyde Waste Liquid Treatment by BZ-001H Intensive Treatment System

[0029] Inoculate 0.3‰ BZ-001H strain (based on dry cell weight) in a conventional activated sludge treatment system (MLSS=2000 ± 100 mg / L) to construct an enhanced treatment system, and add a certain amount of formaldehyde to domestic sewage to make 2000 mg / L, 4000 mg / L, 8000 mg / L formaldehyde sudden pollution accident simulation waste liquid, the simulation experiment is divided into two groups, one group is the control treatment system that maintains the pH change of the treatment system itself, and the second group is every 0.5 h Use 10% NaOH solution to adjust the pH of the treatment system to 6.0-8.0. The treatment system numbers are 1, 2, 30 ℃, 160r / min shaker culture, and samples are taken every 0.5h, 1h, and 2h to determine the pH of each Treat the pH in the system and the formaldehyde content in it.

[0030]

[0031] The results show that the control o...

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Abstract

The invention belongs to the technical field of environment protection and particularly relates to high-efficiency formaldehyde degrading bacteria and use and using method thereof. The number of the high-efficiency formaldehyde degrading bacteria is BZ-001H. The bacteria were approved as Bacilluscereus and were collected in China General Microbiological Culture Collection Center on March 22th, 2010, and the collection number of the bacteria is CGMCCNo.3685. The strain can be used for treating high-concentration formaldehyde waste water and handling emergent formaldehyde sudden pollution accidents. The high-efficiency formaldehyde degrading bacteria have the advantages of quick high-concentration formaldehyde degradation, high tolerance, no secondary pollution, safety for use, no adverse influence on microorganisms in an original sewage treatment system.

Description

technical field [0001] The invention belongs to the technical field of environmental protection, and in particular relates to a high-efficiency degrading bacterium capable of rapidly degrading formaldehyde and its application in biological treatment of high-concentration formaldehyde wastewater and emergency treatment of formaldehyde sudden pollution accidents. Background technique [0002] Formaldehyde is an important chemical raw material, which is widely used in plastics, textiles, synthetic fibers, dyes and other industries. It is the main harmful pollutant and the most common water pollutant in the wastewater discharged by related industries. At present, the treatment of formaldehyde wastewater mainly includes advanced oxidation technologies such as Fenton method, photocatalytic oxidation method, wet oxidation treatment, physical and chemical treatment methods such as chlorine dioxide method, steam stripping, oxidation-adsorption, and biological treatment methods, among...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C02F3/34A62D3/02C12R1/085C02F101/36A62D101/28
Inventor 谭周亮肖波刘庆华周后珍李旭东
Owner CHENGDU INST OF BIOLOGY CHINESE ACAD OF S
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