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Anticancer composition

A composition and compound technology, which is applied in the direction of drug combination, heterocyclic compound active ingredients, antineoplastic drugs, etc., can solve the problems of toxic side effects and unsatisfactory anticancer effects, etc., to reduce toxic side effects, improve inhibitory effect, and enhance curative effect Effect

Inactive Publication Date: 2013-11-06
陈迪 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the anti-cancer effect of these organic compounds is not ideal, and at the same time, it will produce different degrees of toxic side effects

Method used

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  • Anticancer composition
  • Anticancer composition
  • Anticancer composition

Examples

Experimental program
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Effect test

Embodiment 1

[0022] Example 1 Clioquinol, ammonium pyrrolidinedithiocarbamate and disulfiram have the property of binding to copper, zinc, iron and nickel metal elements.

[0023] Experimental method: Clioquinol (CQ), ammonium pyrrolidine dithiocarbamate (PDTC) and disulfiram (DSF) were dissolved in DMSO at a concentration of 40 mmol / L. And with the same concentration of copper chloride (CuCl 2 ), zinc chloride (ZnCl 2 ), ferric chloride (FeCl 2 ), nickel chloride (NiCl 2 ) were mixed in equal amounts, and the final concentration of the mixture was 20 mmol / L. Take 200 μl of the mixture and place it in a 96-well plate. 200 μl of the unmixed above-mentioned single solution (20 mmol / L) was also placed in a 96-well plate as a control. Record its color change with a scanner.

[0024] Experimental results: Mixing clioquinol, ammonium pyrrolidinedithiocarbamate, and disulfiram with four different metal salts at equal molar concentrations found that these mixtures may have different degrees ...

Embodiment 2

[0025] Example 2 With the participation of copper, disulfiram can inhibit the proteasome activity of tumor cells and induce the apoptosis of tumor cells.

[0026] Experimental method: human breast cancer MDA-MB-231 cells were cultured in DMEM / F12 medium containing 10% fetal bovine serum, and placed in a p100 culture dish. When the cells reached 70% density, the cells were treated with 10 μmol / L disulfiram (DSF), copper chloride (Cu) or DSF + Cu for different times. After the cells were harvested, the proteins in the cell lysate were analyzed by electrophoresis, transferred to a membrane, and analyzed by Western-blot with different specific antibodies.

[0027] Experimental results: The research proves that under the same conditions, only when disulfiram and copper co-exist (this experiment is 1:1 equimolar concentration) can it have anti-cancer effect, but disulfiram or copper chloride alone cannot Inhibits proteasome activity and induces tumor cell apoptosis (see figure 2 ...

Embodiment 3

[0028] Example 3 The Chinese herbal medicine significantly improves the inhibitory effect of dithioamide compounds + copper or + zinc on the growth of human tumor cells, and enhances its efficacy in inducing tumor cell apoptosis.

[0029] Experimental method: human breast cancer MDA-MB-231 cells were cultured in DMEM / F12 medium containing 10% fetal bovine serum, and placed in a p100 culture dish. When the cells reached 70% density, treat the cells with 10 μmol / L zinc chloride (Zu), copper chloride (Cu), disulfiram (DSF), or Chinese herbal medicine (Herb, 20 μg / ml) alone, or The same concentration of DSF + Zn, DSF + Cu, DSF + Zn + Herb or DSF + Cu + Herb was used in combination to treat the cells for 24 hours. The MTT method was then used to analyze the inhibitory effects of these treatments on tumor cell proliferation ( image 3 A); or use Western-blot method to analyze the effect of these treatments on the induction of tumor cell apoptosis ( image 3 B). The selected Chine...

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Abstract

The invention relates to an anticancer composition and application thereof. The anticancer composition comprises dithioamides or / and oxyquinolines with metallic element combining capacity, organic or / and inorganic salts containing copper or / and zinc, Chinese herbal medicine extract and pharmaceutically acceptable carrier or solvent, wherein the Chinese herbal medicine comprises at least one of ginseng, radix astragali, wolfberry fruit, mulberry, Rabdosia rubescens, gromwell, radix salviae miltiorrhizae, pseudo-ginseng, peach kernel, eucommia and oldenlandia. The Chinese herbal medicine in the anticancer composition can obviously enhance the inhibiting action of the dithioamides or / and oxyquinolines + copper or / and + zinc on human tumor cells, improve the treatment effect of inducing apoptosis of tumor cells, reduce the toxic and side effect and obviously inhibit tumor growth, thereby enhancing the life quality of the patients with tumors and prolonging the survival time of the patients with tumors.

Description

technical field [0001] The invention relates to the field of medicines, in particular to an anticancer composition and its application. Background technique [0002] Copper, zinc, iron and other metals are essential trace elements for humans and animals. Copper is an important component of many enzyme proteins in the body, such as ceruloplasmin, cytochrome oxidase, superoxide dismutase, anticorrosion oxidase, tyrosinase, etc. Zinc is an important component of hundreds of proteins in the body, including structural proteins, gene transcription factors, and enzyme proteins. Iron is an important component of hemoglobin. Copper and zinc play a key role in the occurrence and development of tumors. Studies have shown that the content of copper in the serum and tumor tissue of patients with various tumors is significantly higher than that of the normal group, these tumors include breast cancer, prostate cancer, lung cancer, liver cancer, brain tumor, gastric cancer, colon cancer...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K31/47A61K36/815A61K45/00A61P35/00A61P35/02A61P35/04A61K33/30A61K33/34
Inventor 陈迪窦庆平
Owner 陈迪
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