Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Glycerol production process based on Candida krusei

A Candida and culture medium technology, applied in the direction of microorganism-based methods, fermentation, microorganisms, etc., can solve the problems of affecting the yield of glycerin extraction, low conversion rate of sugar and sugar, and high cost of water and electricity, so as to save electricity consumption and Cooling water consumption, high quality, and the effect of reducing environmental costs

Active Publication Date: 2011-12-14
上海天源植物制品有限公司
View PDF4 Cites 1 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0009] But this prior art has the following characteristics: (1) after fermentation on the basis of 200g / L initial sugar, the glycerol content is 67.26g / L-71.75g / L, and the fermentation conversion rate is 34%-36%. (2) The fermentation culture temperature is 33°C, the time is 120h, the culture time is slightly longer, and the cost of water and electricity in the fermentation process is higher. (3) 40g / L NaCl is added to the fermentation medium, which is relatively Improving the yield of glycerin will help to some extent, but if the glycerin needs to be purified, it will seriously affect the extraction yield of glycerin. (4) The final product of this technology is not finished glycerin. Glycerin does not need to be extracted after fermentation, and there is no requirement for the quality of glycerin

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Glycerol production process based on Candida krusei
  • Glycerol production process based on Candida krusei
  • Glycerol production process based on Candida krusei

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] Embodiment 1: the acquisition of high temperature resistant Candida krusei

[0031] Screening of mutant strains with high glycerol production

[0032](1) Bacteria suspension preparation and mutagenesis treatment: in 100mL seed medium / 250mL Erlenmeyer flask, 1 ring of slanted fungus, cultured at 30-35°C, 90-120r / min for 16-20h, centrifuged to collect bacteria, sterile Wash several times with normal saline and dilute for later use. Take 4mL of bacterial suspension, irradiate with 15-20W UV lamp power for 10-20s.

[0033] (2) Primary screening and secondary screening: put the mutagenized bacterial suspension into the screening medium containing 400-500g / L glucose and 70-90g / L osmotic agent, wrap in a black paper dish, 35°C-45°C Cultivate at constant temperature for 46-52h, repeat 3 times. Select large colonies for shake flask fermentation, measure biomass, sugar-sweet conversion rate, fermentation time and residual sugar content, and comprehensively screen to determine ...

Embodiment 2

[0039] Example 2: Synthesis of glycerol in a 30L fermenter

[0040] Pick a ring of bacteria from a fresh slant and insert it into the seed medium (20% filling capacity of the Erlenmeyer flask), cultivate it at 35°C-45°C and 120rmp for 16h, after measuring the concentration of the bacteria, press 15% (v / v) The inoculum is connected to 21L sterile fermentation broth / 30L fermenter, the ventilation ratio is 0.75, the fermentation temperature is 35°C-45°C, the cultivation time is 70h, and the cooling water is saved by 40% in the process (compared with the fermentation temperature of 30°C), the mature fermentation broth The glycerol content in the medium is 14% (w / v), and the residual sugar content is 0.2%, wherein the formulations of the seed culture medium and the fermentation medium are as described in this specification. After the fermentation broth is filtered through the membrane, bacteria, colloidal substances, proteins, polysaccharides and monosaccharides are removed step by...

Embodiment 3

[0041] Embodiment 3: Synthetic glycerol in 300L fermentor

[0042] First, use a 30L seed tank for seed liquid culture, pick a ring of bacteria from a fresh slope and insert it into the seed medium (20% filling capacity of the triangular flask), cultivate it at 35°C-45°C, 110rmp for 16h, and measure the bacterial concentration Finally, insert 21L sterile seed culture medium / 30L seed tank according to 25% (v / v) inoculation amount, ventilation ratio 0.5, 35 ℃-45 ℃ culture 10h, after measuring bacterial concentration, according to 10%-15% (v / v) v) Insert 200L fermentation medium / 300L fermenter, stir at 200rpm, ventilation ratio 0.75, fermentation temperature 35°C-45°C, culture time 66h, to obtain 180L mature fermentation broth with glycerol content of 14.5%. After the same membrane filtration and distillation process as in Example 1, 28 kg of 99.2% glycerin was obtained, and 27.3 kg of finished glycerin was obtained by deodorization and decolorization. The product complies with t...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a candida-krusei-based glycerol production process, which belongs to the technical field of microbial glycerol production. The candida krusei strain TS1008 was collected in China Center for Type Culture Collection on August 10, 2011, and the collection number of the strain is CCTCC No.M2011285. The production process can improve the glycerol conversion rate in a fermentation process and the quality of the final glycerol product, and also can save energy and reduce pollutant emission in a production process for realizing energy-saving and emission-reducing production ofsafer high-quality glycerol.

Description

technical field [0001] The invention relates to a method in the technical field of glycerin production by microbial fermentation, in particular to a glycerol production process based on Candida krusei. Background technique [0002] Glycerin, as an important by-product of chemical products and an important raw material of other chemical products, is mainly used in medicine, cosmetics, personal care products, cigarettes and food. With the continuous development of my country's national economy, the market demand for glycerin, which is an important chemical product for the national economy and people's livelihood, continues to grow, especially in the food, cosmetics and pharmaceutical industries. Therefore, the development of domestic production and application of glycerin is of great significance to the development of our society. [0003] Glycerin, also known as glycerol, is a colorless viscous liquid, an important light chemical raw material, and has a wide range of uses, o...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): C12N1/16C12N13/00C12N15/01C12P7/20C12R1/72
Inventor 姜耀明史其峰
Owner 上海天源植物制品有限公司
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com