Novel extraction method of cerebroprotein hydrolysate solution
A technology of brain protein hydrolyzate and extraction method, which is applied in the fields of hydrolyzed protein components, drug combinations, nervous system diseases, etc., and can solve problems such as tryptophan content not meeting quality standards, incomplete hydrolysis, and identification items not meeting regulations, etc.
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Embodiment 1
[0025] A method for extracting cerebroprotein hydrolyzate solution, comprising the following steps:
[0026] ①. Take fresh or frozen porcine brain, remove the cerebellum and brainstem, and remove the fascia on the surface of the brain tissue, wash it with purified water, add 1 times the purified water colloid and grind for homogenization 3 times.
[0027] ② Take the homogenate, heat it to 45°C, adjust the pH value to 7.5 with sodium hydroxide, add 2.5% trypsin, and stir for 2 hours. After the reaction, the pH value was adjusted to 2.5 with hydrochloric acid, heated to 95° C., kept for 30 minutes, and the supernatant was collected by centrifugal filtration.
[0028] ③. Take the centrifuged supernatant, add 50wt% hydroxylamine solution in a volume ratio of 1:1, adjust the pH value to 2.5 with hydrochloric acid, and react in a closed manner at 80°C for 30 hours. After the reaction, cool to 30°C and adjust the pH value with sodium hydroxide To 7.5, centrifuge to get the supernata...
Embodiment 2
[0031] A method for extracting cerebroprotein hydrolyzate solution, comprising the following steps:
[0032] ①. Take fresh or frozen pig brain, remove the cerebellum and brainstem, and remove the fascia on the surface of the brain tissue, wash it with water for injection, add 2 times purified water or water for injection colloid mill to homogenize twice;
[0033] ② Take the homogenate, heat it to 35°C, adjust the pH value to 8.5, add 0.5% trypsin, stir and react for 8 hours; adjust the pH value to 2.5, heat to 94°C, keep warm for 10 minutes, and centrifuge;
[0034] ③. Take the centrifuged supernatant in step ②, add 40wt% hydroxylamine solution at a volume ratio of 3:1, adjust the pH value to 2.5, and react in a closed manner at 90°C for 20 hours; cool to 33°C, adjust the pH value to 7.2, and centrifugally filter;
[0035] ④, take step ③ centrifuged supernatant, ultrafiltration with ultrafiltration membrane with a molecular weight of 8KD, and collect the filtrate; according to...
Embodiment 3
[0037] A method for extracting cerebroprotein hydrolyzate solution, comprising the following steps:
[0038] ①. Take fresh or frozen pig brain, remove the cerebellum and brainstem, and remove the fascia on the surface of the brain tissue, wash it with water for injection, add 1.5 times of purified water or water for injection colloidal grinder for 3 times;
[0039] ② Take the homogenate, heat it to 40°C, adjust the pH value to 8.0, add 1% trypsin, stir and react for 5 hours; adjust the pH value to 2.2, heat to 98°C, keep warm for 20 minutes, and centrifuge;
[0040] ③. Take the centrifuged supernatant in step ②, add 45wt% hydroxylamine solution at a volume ratio of 1:3, adjust the pH value to 2.0, and react in a closed manner at 92°C for 15 hours; cool to 37°C, adjust the pH value to 6.9, and centrifugally filter;
[0041] ④. Take the centrifuged supernatant from step ③, ultrafilter with an ultrafiltration membrane with a molecular weight of 12KD, and collect the filtrate; acc...
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