Method for promoting to biosynthesize 1,3-propylene glycol by adding reducing sugar

A technology for biosynthesis, propylene glycol, used in microorganism-based methods, biochemical equipment and methods, microorganisms, etc.

Active Publication Date: 2012-01-11
CHINA PETROLEUM & CHEM CORP +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Different additives can promote the microbial fermentation process to a certain extent, but the product concentration, long growth cycle and glycerin conversion rate of the microbial fermentation process can be further improved

Method used

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  • Method for promoting to biosynthesize 1,3-propylene glycol by adding reducing sugar
  • Method for promoting to biosynthesize 1,3-propylene glycol by adding reducing sugar
  • Method for promoting to biosynthesize 1,3-propylene glycol by adding reducing sugar

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0014] Embodiment 1 Adding xylose to increase reducing equivalent is fermented in shake flask

[0015] Bacterial species: Klebsiella pneumoniae (Klebsiella pneumoniae) was screened by Fushun Petrochemical Research Institute, and preserved in China Microorganism Culture Collection Center (CGMCC), No. 0798. LB solid medium: Each liter of medium contains peptone 10.0g / L, yeast extract 5.0g / L, NaCl 10.0g / L, pH 7.0.

[0016] Seed medium: yeast extract 5.0g / L, maltose 3.0g / L, peptone 5.0g / L, NaCl 5.0g / L, pH 7.0.

[0017] Fermentation medium: yeast extract 5.0g / L, K 2 HPO 4 ·3H 2 O 10g / L, KH 2 PO 4 2g / L, NH 4 Cl 1g / L, NaCl 0.5g / L, MgSO 4 ·7H 2 O 0.1g / L, FeCl 3 ·6H 2 O 30mg / L, CoCl 2 ·6H 2 O5mg / L, Vitamin B 12 (Vitamin B 12 ) 5mg / L, glycerol (glycerin) 30g / L, pH 7.0, reducing sugar (xylose, mannose, rhamnose, etc.) concentration is 0-20g / L.

[0018] Fermentation process:

[0019] Step 1: Activation of strains

[0020] Pick a ring of Klebsiella pneumonia from the glyc...

Embodiment 2

[0035] Example 2 Adding xylose to increase reducing equivalent is fermented in a 7 liter fermenter

[0036] During the fermentation process, the seed solution was inserted into a 7-liter fermenter, and the filling capacity was 4 liters. The fermentation medium contains a glucose concentration of 5g / L, an inoculum size of 5%, microaerobic fermentation (0.25vvm air), 37°C, 300rpm. All the other conditions refer to Example 1. Samples were taken every 6 hours, and the measured items included bacterial cell concentration, substrate glycerin concentration, synthesis concentration of 1,3-propanediol and concentration of other main by-products.

[0037] Experiment No. 1 added xylose every 6 hours after fermentation for 6 hours, adding xylose each time to make the fermentation system contain 4g / L xylose.

[0038] Experiment No. 2 added glucose every 6 hours after fermentation for 6 hours, adding glucose each time to make the fermentation system contain 4 g / L of glucose.

[0039] Exp...

Embodiment 3

[0044] Embodiment 3 adds mannose in fermentation medium

[0045] In a 250mL shake flask equipped with 100mL of fermentation medium (the mannose content is 10g / L), the inoculum size was 2%, 37°C, 140°C, and the incubation time was 30h. All shake flasks are narrow-mouth bottles with rubber stoppers. Sampling was taken every 5 hours in the middle, and the volume of each sampling was 4mL. The measured items included bacterial cell concentration, substrate glycerol and mannose concentration, synthesis concentration of 1,3-propanediol and concentration of other main by-products. For strain activation and seed cultivation, refer to Example 1. The experimental results are shown in Table 3.

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Abstract

The invention relates to a method for promoting to biosynthesize 1,3-propylene glycol by adding reducing sugar, which comprises the following steps: preparing a fermentation medium used for the 1,3-propylene glycol; by taking glycerol as a fermentation substrate, inoculating a Klebsiella pneumonia strain culture solution into the fermentation medium to carry out anaerobic fermentation or microaerophillic fermentation to synthesize the 1,3-propylene glycol; and in the fermentation process, introducing the reducing sugar of which the fermentation system concentration is 1-50g / L. In the method for promoting to biosynthesize the 1,3-propylene glycol by adding the reducing sugar, according to the characteristic of xylose, mannose, rhamnose and the like producing a large number of reducing equivalents in the metabolic pathway in a bacterium body, by utilizing the principle that the biosynthesis of the 1,3-propylene glycol can be promoted because of the existence of more reducing equivalents, a good amount of reducing sugar is added to the fermentation medium to increase the reducing equivalents, and thereby, the synthesis concentration and the conversion rate of the 1,3-propylene glycolare improved. The method for promoting to biosynthesize the 1,3-propylene glycol by adding the reducing sugar is simple to operate, is capable of improving the bioavailability and reducing the fermentation cost and is suitable for industrial production.

Description

technical field [0001] The invention relates to a method for synthesizing 1,3-propanediol by microbial fermentation, in particular to a method for producing 1,3-propanediol by microbial fermentation using glycerol as a substrate. Background technique [0002] 1,3-Propanediol (PDO) is an important chemical and pharmaceutical intermediate raw material, which can be used as an organic solvent in ink, printing and dyeing, emulsifier, antifreeze, etc. The main purpose of PDO is to synthesize the monomer of new polyester polymer material polytrimethylene terephthalate (PTT). PTT is currently one of the six new petrochemical products recognized internationally. Compared with polyethylene terephthalate (PET), polybutylene terephthalate (PBT) and nylon, PTT has good printing and dyeing properties, high elasticity, anti-ultraviolet, good static performance, biodegradable and Recycling and other excellent properties, so PTT has very broad application prospects in many fields such as ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P7/18C12R1/22
Inventor 金平王领民师文静李晓姝王崇辉
Owner CHINA PETROLEUM & CHEM CORP
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