Preparation method of lactic acid Amphibacillus fermentation liquor, and preparation method of lactic acid Amphibacillus fermentation liquor composite antiseptic by using fermentation liquor thereof

A compound preservative and bacterial fermentation technology, which is applied in the field of preparation of bibacillus lactic acid fermentation liquid, can solve the problems of high production cost of preservatives, complicated preparation process, and low output, and achieve low cost, simple preparation process, and high output Effect

Inactive Publication Date: 2013-03-13
SUIHUA LANYUAN BIOLOGICAL ENG CO LTD
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  • Claims
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Problems solved by technology

[0004] The present invention aims to solve the problems of complex preparation process of the existing bibacillus lactic acid, high production cost and low yield in the preparation of its antiseptic, and provides a method for preparing a bibacillus lactic acid fermentation liquid and using the fermentation liquid to prepare double lactic acid buds Method for compounding antiseptic in bacterial fermentation liquid

Method used

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  • Preparation method of lactic acid Amphibacillus fermentation liquor, and preparation method of lactic acid Amphibacillus fermentation liquor composite antiseptic by using fermentation liquor thereof
  • Preparation method of lactic acid Amphibacillus fermentation liquor, and preparation method of lactic acid Amphibacillus fermentation liquor composite antiseptic by using fermentation liquor thereof
  • Preparation method of lactic acid Amphibacillus fermentation liquor, and preparation method of lactic acid Amphibacillus fermentation liquor composite antiseptic by using fermentation liquor thereof

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specific Embodiment approach 1

[0010] Specific embodiment one: the preparation method of the lactic acid bibacterium fermented liquid of the present embodiment, carry out according to the following steps: one, respectively activate Lactococcus lactis, thermophilic alicyclic acid bacillus, globular bacillus and two spore zygosacillus, Get the activated bacterium liquid of four kinds of bacteria; 2, extract the thalline of four kinds of bacterium from the activated bacterium liquid of four kinds of bacterium; 1:1 mixed, then inoculated in a fermenter equipped with a fermentation medium, the inoculum amount is 8% to 12% (volume), at a fermentation temperature of 29 to 31°C and a shaking table speed of 100 to 150r / min Ferment under the conditions for 45-50 hours to obtain a fermentation liquid; 4. Place the fermentation liquid on a shaker at 100-150 r / min and cultivate for 45-50 hours to complete the preparation of the bibacterium lactis fermentation liquid.

[0011] In this embodiment, the preparation process ...

specific Embodiment approach 2

[0035]Specific embodiment two: the difference between this embodiment and specific embodiment one is that the method for activating Lactococcus lactis, Alicyclobacillus thermophiles, Bacillus coccoidus and Zygomyces dispora respectively in step one is: the concentration The Lactococcus lactis bacterial liquid of 0.05~0.15g / kg is inoculated in the culture medium A according to the inoculum amount of 8%~12% (volume), and the concentration is 0.1~0.2g / kg Alicyclobacillus thermophilic bacteria The solution was inoculated in medium B at an inoculum amount of 8% to 12% (volume), and the Bacillus bacteria solution with a concentration of 0.05 to 0.15g / kg was inoculated in the culture medium at an inoculum amount of 8% to 12% (volume). In base C, inoculate Zygomyces bispores with a concentration of 0.2-0.4 g / kg into medium D at an inoculum size of 8%-12% (volume), and then cultivate them in a constant temperature incubator at 28-32°C 45~50h, the activation is complete; every 200mL med...

specific Embodiment approach 3

[0037] Specific embodiment three: the difference between this embodiment and specific embodiment one or two is: the method for extracting the thalli of four kinds of bacteria from the activated bacterium liquid of four kinds of bacteria in step 2 is: the activated bacterium liquid of four kinds of bacteria is extracted Centrifuge separately, the activated bacterial liquid of Lactococcus lactis, the activated bacterial liquid of Alicyclobacillus thermophile and the activated bacterial liquid of Bacillus cocciformis are centrifuged at 2500-3500r / min for 25-35min, and the activated bacterial liquid of Zygomyces dispora is centrifuged at 2500-3500r / min Min centrifuge for 10-20min, then collect the four kinds of bacteria respectively, then wash the four kinds of bacteria with 0.4mol / L, pH value of 7.5 phosphate buffer solution for 3-5 times, collect the bacteria by centrifugation, and obtain four kinds of bacteria of bacteria. Others are the same as in the first or second embodimen...

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Abstract

The invention provides a preparation method of lactic acid Amphibacillus fermentation liquor, and a preparation method of a lactic acid Amphibacillus fermentation liquor composite antiseptic by using the fermentation liquor thereof. The method of the invention aims at solving the problems of present complex preparation technology of lactic acid Amphibacillus as well as high production cost and low output existed in antiseptic preparation. The preparation method for the fermentation liquor comprises the following steps: activating bacteria liquid; extracting thallus from the activated bacteria liquid of four types bacterium; mixing the thallus, inoculating into a fermentation cylinder for fermenting to obtain the fermentation liquor; culturing the fermentation liquor to finish the preparation of the fermentation liquor. The method for preparing the composite antiseptic comprises the following steps: mixing nisin, natamycin, propionic acid, bamboo leaf antioxidant, chitosan, disodium ethylene diamine tetraacetate, ethanol, tocopherol and activated water; mixing the mixed liquor and the fermentation liquor to react to finish the preparation of the composite antiseptic. The method of the invention has the advantages of simple preparation technology, low cost and high output. The composite antiseptic is used for food antiseptic.

Description

technical field [0001] The invention relates to a method for preparing a bibacillus lactic acid fermented liquid and a method for preparing a bibacillus lactic acid fermented liquid compound preservative by using the bibacillus lactic acid fermented liquid. Background technique [0002] Bibacillus lactic acid is a biological food preservative, which has a wide range of bactericidal and antibacterial capabilities, and the effect is very good. The antibacterial mechanism is to establish a mode of enzyme biosynthesis and activity regulation, and form micropores on the cell membrane of microorganisms, resulting in increased membrane permeability, energy system damage, and food toxin damage, ensuring that no allergens are produced in food, so it can quickly kill Destroy microorganisms in food, and inhibit a small amount of microorganisms in food to avoid their reproduction and immune reaction of water-soluble glycoproteins, so as to achieve the purpose of antiseptic preservation....

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/00C12R1/01C12R1/07C12R1/645
Inventor 王心礼
Owner SUIHUA LANYUAN BIOLOGICAL ENG CO LTD
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