Virus preparation or vaccine production method by culturing cells with polyester fiber carrier

Abstract

The invention relates to a virus preparation or vaccine production method by culturing cells with a polyester fiber carrier. The method particularly comprises the following steps: 1) preparing a polyester fiber cell culturing carrier by adopting a processing method; 2) in a cell culturing system, culturing proliferation cells in a cell proliferation culture solution with the treated polyester fiber cell culturing carrier; 3) changing a cell maintaining culture solution after the cells grow to a certain density or number, and inoculating virus to infect cells; 4) amplifying virus; 5) harvesting virus fluid; 6) inactivating or implementing living vaccine treatment according to the required preparation type; 7) concentrating, purifying and cracking the virus fluid; 8) adding a stabilizing agent and an immunoadjuvant; and 9) packaging vaccine. According to the method, a material which has low price and is suitable for wall attaching growth of the cells is used to make the cell culturing carrier, carry out cell culture and prepare virus or produce vaccine, so that the cost of the cell carrier is greatly reduced.

Description

technical field [0001] The invention relates to the field of biotechnology and pharmacy, in particular to a method for cultivating cells on a polyester fiber carrier to prepare viruses or produce vaccines. Background technique [0002] Vaccines are autoimmune preparations made from pathogenic microorganisms (such as bacteria, rickettsia, viruses, etc.) and their metabolites, which are artificially attenuated, inactivated, or genetically engineered to prevent infectious diseases. Vaccine, as a special drug, is a biological product produced by the joint development of immunological experience theory and biotechnology. It eliminates the threat of many infectious diseases to animal life from the perspective of preventive measures, ensures human health, and promotes the development of human health. The healthy development of the breeding industry has played a huge role in the healthy development of the national economy and the people's livelihood. [0003] The discovery of vacci...

AI Technical Summary

Problems solved by technology

Currently commonly used commercial microcarriers include Cytodex series, Biosilon series, Solo microcarriers, etc. These microcarriers are often too expensive for cell culture to prepare viruses or produce vaccines, and are not suitable for large-scale production. Carry out industrialized production; at the same time, as a molded commodity, the shape and size of these cell carriers have been fixed and are not easy to change, which affects the design, improvement and optimization of the corresponding process

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