Method for detecting eighteen-component codonopsis pilosula preparation

A detection method and preparation technology, applied in the detection field of traditional Chinese medicine preparations

Active Publication Date: 2012-04-18
TIBET QIZHENG TIBETAN MEDICINE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0015] The current quality inspection standard only uses microscopes for identification. The specificity and reproducibility of this detection method need to be further improved. In addition, this detection method does not include limited inspection of toxic ingredients in the prescription

Method used

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  • Method for detecting eighteen-component codonopsis pilosula preparation
  • Method for detecting eighteen-component codonopsis pilosula preparation
  • Method for detecting eighteen-component codonopsis pilosula preparation

Examples

Experimental program
Comparison scheme
Effect test

experiment example 1

[0095] Experimental example 1: Microscopic identification

[0096] Take the eighteen-flavored Codonopsis honey pill, grind it finely, add chloral hydrate to permeabilize it for 1-3 times, make a tablet, observe under a microscope, and identify the morphological characteristics of each medicinal material:

[0097] There are nodules connecting the milk ducts, with a diameter of 12-15 μm, and the cells in and around the milk ducts are full of oil droplets and fine particles. Inulin is fan-shaped, with radial texture on the surface (Tibetan Codonopsis). There are many starch granules, the single granule is spherical, polygonal or helmet-shaped, with a diameter of 4-11 μm, the umbilical point is obvious, and it is dot-shaped, star-shaped or fissure-shaped, and the compound granules are composed of 2-5 granules (Bangna). The testa palisade cells are mostly in sheets, colorless or light yellow, sub-polygonal in surface view, and the wall is slightly shrunken (Cassiae). The fibers a...

experiment example 2

[0098] Experimental example 2: TLC identification of woody fragrance

[0099] (1) Instrument

[0100] Mortar, electronic scale, Erlenmeyer flask with stopper, pipette, ultrasonic extractor, funnel, filter paper, evaporating dish, sample spotter, silica gel G thin-layer plate, double-slot chromatography cylinder, spray bottle, hair dryer.

[0101] (2) Control medicinal materials

[0102] Dehydrocressin as reference drug (batch number: 111525-200907) was purchased from China National Institute for the Control of Pharmaceutical and Biological Products.

[0103] (3) Reagents

[0104] Methanol, acetone, cyclohexane, dichloromethane, ethyl acetate, 5% vanillin sulfuric acid solution.

[0105] (4) Inspection method:

[0106] Selection of extraction solvent: methanol and acetone are respectively used as extraction solvents;

[0107] Selection of extraction methods: ultrasonic treatment and heating reflux respectively;

[0108] The choice of developing solvent: cyclohexane-dichlo...

experiment example 3

[0113] Experimental example 3: TLC identification of Tibetan calamus

[0114] (1) Instrument

[0115] Mortar, electronic scale, Erlenmeyer flask with stopper, pipette, ultrasonic extractor, funnel, filter paper, evaporating dish, spotter, silica gel GF 254 plates, chromatographic cylinders, and UV spotting analyzers.

[0116] (2) Control medicinal materials

[0117] Tibetan calamus control medicinal material (batch number: 121084-200502) was purchased from China Institute for the Control of Pharmaceutical and Biological Products.

[0118] (3) Reagents

[0119] Methanol, ether, petroleum ether (60℃~90℃), ethyl acetate, formic acid.

[0120] (4) Inspection method:

[0121] Selection of extraction solvent: using methanol and ether as extraction solvent;

[0122] Selection of extraction methods: respectively adopt heating reflux and ultrasonic extraction;

[0123] The choice of developer: petroleum ether (60℃~90℃)-ethyl acetate (5:1), petroleum ether (60℃~90℃)-ethyl acetate...

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Abstract

The invention relates to a detecting method for an eighteen-component codonopsis pilosula preparation, which comprises one or more of the following identification and/or detection methods: identifying the morphological characteristics of medical materials such as Tibetan codonopsis pilosula, Bangna (a traditional Chinese medicament), cassia seed, Tibetan drug sweetflag rhizome, Chinese tinospora stem, denucleated medicament terminalia fruit, gymnadenia conopsea, fructus terminaliae billericae, frankincense, Kukuinabu (a traditional Chinese medicament) and Baxiaga (a Traditional Tibetan medicament containing vasicine) in the preparation through a microscope; qualitatively identifying costustoot, the Tibetan drug sweetflag rhizome and the frankincense in the preparation through thin layer chromatography; and detecting the content of aconitine in the preparation. By the method, the eighteen-component codonopsis pilosula preparation can be effectively detected.

Description

technical field [0001] The invention belongs to the technical field of traditional Chinese medicines, and in particular relates to a detection method of traditional Chinese medicine preparations. Background technique [0002] Shibawei Dangshen Pills is a traditional prescription of Tibetan medicine. Composed of ambrette seed, Kuquinab, Songsheng ointment, Pashaga, Emlical seed and woody eighteen medicinal materials, it has the effects of anti-inflammatory and pain relief, healing sores and ulcers, and removing yellow water. For arthralgia, "Gamba" disease, redness and pain in extremities and joints, difficulty in stretching and flexion, eczema, psoriasis, pitting ringworm, leprosy, amaziosis and leprosy. [0003] The quality inspection standards of the currently used Shibawei Dangshen Pills are recorded in the first volume of Tibetan Medicine Standards of the Ministry of Health: [0004] Shibawei Dangsheng Wan, pinyin name: Shibawei Dangsheng Wan [0005] Page number: C1-...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/90
Inventor 陈丽娟张国霞
Owner TIBET QIZHENG TIBETAN MEDICINE
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