Ternary composite organic pollution repairing agent containing iron oxide, humus and reducing bacteria thereof, and preparation method of repairing agent

A technology of organic pollution and ternary compounding, which is applied in chemical instruments and methods, organic fertilizers, biological sludge treatment, etc., can solve the problems of preparing ternary compound restoration agents without discovering iron oxides, humus and reducing bacteria, etc. Achieve good in-situ repair effect, accelerate the degradation of organic pollutants, and reduce the amount of engineering

Active Publication Date: 2012-05-09
GUANGDONG INST OF ECO ENVIRONMENT & SOIL SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] After literature and patent searches, no reports were found on the use of iron oxides, humus and reducing bacteria to prepare ternary composite remediation agents, and there are precedents for the application of organic pollution remediation in soil and river sediments

Method used

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Examples

Experimental program
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preparation example Construction

[0031] A preparation method of a ternary composite organic pollution remediation agent, comprising the steps of:

[0032] 1) Preparation of medium: Add 3-6g of beef extract, 8-13g of peptone, and 9-14g of sodium chloride per liter of water to prepare the medium, adjust its pH to 7.0-7.5, and sterilize it to obtain a liquid medium ,spare;

[0033] 2) Shake flask culture: inoculate the strain according to claim 1 into a shake flask containing the above-mentioned liquid medium, and perform aerobic culture at 28 to 35° C. to obtain a shake flask bacterial liquid;

[0034] 3) Seed tank fermentation: Inoculate the shake flask bacterial liquid into the seed tank according to the inoculum amount of 1% to 5%, and carry out fermentation and cultivation to obtain the seed liquid;

[0035] 4) Fermentation tank fermentation: inoculate the seed liquid into the fermentation tank according to the inoculation amount of 1% to 5%, and obtain the fermentation liquid;

[0036]5) Mixing treatment...

Embodiment 1

[0041] Preparation of three-component composite restoration agent of goethite, humus and humus-reducing Corynebacterium

[0042] (1) culture medium

[0043] Beef extract 5g, peptone 10g, sodium chloride 10g, tap water 1000mL, add antifoaming agent XPJ960 containing silicon foam, adjust pH to 7.5, sterilize at 121℃ for 30 minutes under 15 pounds of pressure and set aside.

[0044] (2) Shake flask culture

[0045] Corynebacteria were restored from normal preserved humus ( Corynebacterium humireducens MFC5) pick a loop on the slant culture medium and inoculate it into the Erlenmeyer flask containing the bacterial culture medium for shaking flask culture. The conditions are: temperature 35°C, rotation speed 250 rpm, volume of liquid in the triangle bottle 30%, incubation time 16 hours;

[0046] (3) Seed tank fermentation

[0047] According to the inoculum amount of 3%, the shake flask bacterial liquid of Corynebacterium humus reducing was transferred into the seed tank for fer...

Embodiment 2

[0057] Preparation of three-component composite restoration agent of ferrihydrite, humus and Pantoea agglomerans

[0058] (1) culture medium

[0059] Beef extract 6g, peptone 9g, sodium chloride 9g, tap water 1000mL, add antifoaming agent polyether antifoaming agent GPE according to 0.05% by weight, adjust pH to 7.0, fully dissolve, stir well, put in seed tank or fermentation tank Sterilize at 121°C for 40 minutes under 15 pounds of pressure, and cool to 30°C for later use.

[0060] (2) Shake flask culture

[0061] From preserved normal Pantoea agglomerans ( Pantoea agglomerans MFC3 ) on the slant culture medium to inoculate a ring into a Erlenmeyer flask containing bacterial culture medium for shaking flask culture. The conditions are: temperature 30°C, rotation speed 200 rpm, volume of liquid in the triangular flask 20%, and incubation time 20 hours.

[0062] (3) Seed tank fermentation

[0063] Transfer the shake flask bacterial solution of Pantoea agglomerans into the...

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PUM

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Abstract

The invention discloses a ternary composite repairing agent, containing iron oxide, humus and reducing bacteria thereof, for in-situ remediation of organic pollution in soil and sediment. The repairing agent of the invention is prepared by carrying out fermentation enlarge culture on ferric reducing microorganism and mixing with iron oxide and humus uniformly. The microbial repairing agent of the invention can accelerate degradation of organic pollutants under an anaerobic condition and has good in-situ remediation effect on organic pollution soil and black and stink sediment. Raw materials are all natural substances and environment-friendly, and cause no secondary pollution. The microbial repairing agent of the invention is simply applied without excavating or disturbing original environment or oxygen, requires low work amount and small cost, and is apt to large-scale usage. The preparation method of the repairing agent of the invention can obtain a microbial repairing agent product simply and fast.

Description

technical field [0001] The invention relates to an organic pollution remediation agent and a preparation method thereof, in particular to a ternary composite remediation agent of iron oxide, humus and its reducing bacteria which can be applied to in-situ restoration of organic pollutants in soil and river bottom mud and its preparation method. Background technique [0002] In recent years, with the extensive use of chemical fertilizers and pesticides, a large amount of organic pollutants such as petroleum, polycyclic aromatic hydrocarbons, and organic chlorine have been discharged, resulting in increasingly serious organic pollution of soil and water in my country. Organic pollutants in soil and water enter the human body from the food chain through the soil-plant system and farmland irrigation, directly endangering human health. Therefore, the research on the remediation technology of organic polluted soil and water body has become a hot spot in environmental protection re...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C09K17/40C02F11/02C09K101/00
CPCY02W10/20
Inventor 周顺桂李芳柏徐荣险王跃强
Owner GUANGDONG INST OF ECO ENVIRONMENT & SOIL SCI
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