Bacillus amyloliquefaciens and application thereof

A technology of amylolytic spores and bacilli, applied in the fields of application, bacteria, and fungicides, can solve the problems of general biocontrol effects, single biocontrol objects, and unsuitable extraction, and achieve broad-spectrum plant pathogenic bacteria resistance and stable antibacterial Efficient effect, antibacterial range

Inactive Publication Date: 2012-06-06
中国农业科学院研究生院
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] It has been reported that the antibacterial substances synthesized by Bacillus amyloliquefaciens with biocontrol activity are extracellular substances, the concentra

Method used

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  • Bacillus amyloliquefaciens and application thereof
  • Bacillus amyloliquefaciens and application thereof
  • Bacillus amyloliquefaciens and application thereof

Examples

Experimental program
Comparison scheme
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Example Embodiment

[0017] Example 1. Obtainment and identification of Bacillus amyloliquefaciens YB-3 of the present invention

[0018] 1. Sample collection

[0019] Collect soil samples from the garden of the Chinese Academy of Agricultural Sciences.

[0020] 2. Isolation and resistance screening of strains

[0021] (1) Dilution of strains: mix the sample taken evenly, weigh 1g and dissolve it into an Erlenmeyer flask containing 10ml of distilled water, shake vigorously to make 10 -1 The soil suspension, then use a 1ml pipette to pipette 1ml of the suspension into a test tube containing 9ml of distilled water, shake well to make 10 -2 Soil suspension, diluted to 10 -8 ;

[0022] (2) Preparation of culture medium: Sterilize the prepared NA medium and PDA medium and pour them into a sterile petri dish to cool. The composition of NA medium is 10g peptone, 5g beef extract, 5g NaCL, 18g agar powder, Distilled water 1000ml, PH7.0; PDA medium components are glucose 20g, peeled potato 200g, agar powder 18g, dis...

Example Embodiment

[0033] Example 2: Determination of the antibacterial spectrum of Bacillus amyloliquefaciens YB-3

[0034] Select common plant disease pathogens in agricultural production: Rhizoctonia solani ACCC 36441, Phytophthora capsici ACCC36278, Cotton Fusarium ACCC 36879, Gibberella wheat ACCC 31059, Botrytis cinerea ACCC30387, Sclerotinia sclerotiorum ACCC 36084, Aspergillus oryzae ACCC36443 , Soybean root rot fungus ACCC36242, soybean anthracnose ACCC36201, lettuce sclerotium ACCC36081, cucumber anthracnose ACCC36065, cucumber gray mold ACCC 36448, rice blast fungus ACCC 37632, cotton verticillium wilt ACCC30309 (the above strains are all from Chinese agricultural microorganisms Preservation Management Center) as an indicator bacteria, using the mycelial growth rate method to determine its antibacterial effect. The specific method is as follows: under aseptic conditions, 0.05ml of Bacillus amyloliquefaciens YB-3 culture solution (NB medium 37 ℃ to OD 660 0.7, NB medium components are pep...

Example Embodiment

[0039] Example 3 Detection of Bacillus amyloliquefaciens (Bacillus amyloliquefaciens) YB-3 antibacterial substance distribution

[0040] Bacillus amyloliquefaciens (Bacillus amyloliquefaciens) YB-3 was cultured at 180rpm and 37℃ for 16 hours (medium composition: beef extract 5g, peptone 8g, MgSO 4 1.4g, CaCO 3 0.8g, NaCL5g, 1000ml of distilled water, pH6.8, 1000ml of distilled water), then filter the culture solution with a 0.22μm sterile filter to collect the filtrate and bacteria.

[0041] Under aseptic operation, spread the culture solution of Rhizoctonia solani and Phytophthora capsici (PD medium, 150rpm, cultured at 25°C for 4 days) on the PDA plate, and then use a sterile punch (6mm) on the plate Punch several holes, add the collected bacteria (a ring of bacteria) and the filtrate (50 microliters) into the holes respectively, and place them in an incubator at 25°C for 7 days.

[0042] The results show that there is no antagonistic active ingredient in the fermentation broth of ...

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Abstract

The invention discloses Bacillus amyloliquefaciens and an application thereof. The Bacillus amyloliquefaciens is Bacillus amyloliquefaciens YB-3, and the preservation number of the Bacillus amyloliquefaciens YB-3 in China General Microbiological Culture Collection Center (CGMCC) is CGMCC No.4318. The Bacillus amyloliquefaciens YB-3 which can generate antibiotic substances which only exist in cells and are not diffused to a fermentation liquid and has the advantages of broad spectrum antibiotic range, and efficient and stable antibiotic effect can be developed into a microbe biocontrol preparation with various functions in the future.

Description

technical field [0001] The present invention relates to a strain of Bacillus amyloliquefaciens and its application, in particular to a newly screened strain of Bacillus amyloliquefaciens YB-3 (preservation number: CGMCC NO.4318), and the use of the strain in preventing and treating pathogenic fungi that can cause plant diseases Applications. Background technique [0002] In agricultural production, huge losses are caused by pests and diseases every year. Chemical pesticides have the advantages of high control effect, fast speed, wide insecticidal and bactericidal spectrum, low cost and easy use, so they have always played a leading role in the prevention and control of agricultural diseases and insect pests. Balance poses a great threat. [0003] In recent years, as people's demand for pollution-free, pure natural green food has increased, various developed countries have successively formulated development plans to ban chemical pesticides. Biopesticides have been widely ...

Claims

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Application Information

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IPC IPC(8): C12N1/20A01N63/02A01P3/00C12R1/07
Inventor 王海胜朱晓飞胡元森闫艳春许雷
Owner 中国农业科学院研究生院
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