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Method for preparing composite calcium bilirubinate from ox gall

A technology for compounding bilirubin calcium and ox bile, which is applied in the field of manufacturing compound bilirubin calcium and intermediates from ox bile, which can solve the problems of complex process and low efficiency, improve product yield and simplify the production process Effect

Active Publication Date: 2012-06-27
WUHAN JIANMIN DAPENG PHARMA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] In the existing synthetic bezoar technology, it is generally used to inoculate Escherichia coli into bile, change the physical and chemical properties of bile through microbial fermentation, and then add saturated lime water to obtain compound bilirubin calcium. This process is not only complicated, but also And the efficiency is extremely low. In fact, the process of microbial fermentation is the process of producing β-glucuronidase and adjusting the pH value of bile, and we can replace these two points with a new method, so as to omit the complexity of fermentation. Operation, the present invention is exactly under the premise that the composition of compound bilirubin calcium is guaranteed to be stable, further simplify the production process, and improve the product yield

Method used

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  • Method for preparing composite calcium bilirubinate from ox gall
  • Method for preparing composite calcium bilirubinate from ox gall

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0015] (1) Adjust the content of taurine in natural ox bile to 3% (g / ml).

[0016] (2) The Escherichia coli liquid in the stable growth phase is subjected to ultraviolet sterilization treatment to kill the bacteria, and ultrasonic vibration is performed to rupture the bacteria, and 10000ml of ox bile is mixed with the strain treatment solution at a ratio of 1 to 1. Stir and keep warm at 30°C for 1.5 hours, adjust the pH value of the mixture to 5, stir and keep warm at 30°C for 1.5 hours, then add 11000ml of clarified saturated calcium oxide solution, stir well, and filter out the brown-red precipitate

[0017] (3) Add 350g bilirubin, 50g cholic acid, 25g deoxycholic acid, 3g zinc sulfate, and 3g magnesium sulfate to every 569g (dry weight) of the precipitate, stir and mix well for later use.

[0018] (4) Put the prepared compound bilirubin calcium into a low-temperature freezer, freeze at -5°C for 24 hours, then transfer it to a vacuum drying oven, vacuumize to 0.085Mpa, and ...

Embodiment 2

[0020] (1) Adjust the content of taurine in natural ox bile to 5% (g / ml).

[0021] (2) The Enterococcus bacterial liquid in the stable growth stage is subjected to ultraviolet sterilization treatment to kill the bacterial cells, and ultrasonic vibration is performed to rupture the bacterial cells, and 10000ml of ox bile is mixed with the strain treatment solution at a ratio of 2 to 1. Stir and keep warm at 40°C for 1 hour, adjust the pH value of the mixture to 4, stir and keep warm at 40°C for 1 hour, then add 30,000ml of clarified saturated calcium oxide solution, stir well, and filter out the brown-red precipitate

[0022] (3) Add 450g bilirubin, 100g cholic acid, 50g deoxycholic acid, 5g zinc sulfate, and 5g magnesium sulfate to every 390g (dry weight) of the precipitate, stir and mix well for later use.

[0023] (4) Put the prepared compound bilirubin calcium into a low-temperature freezer, freeze at -8°C for 20 hours, then transfer it to a vacuum drying oven, evacuate t...

Embodiment 3

[0025] (1) Adjust the content of taurine in natural ox bile to 7% (g / ml).

[0026] (2) Ultraviolet sterilizing treatment is carried out on the Proteus bacterial liquid in the stable growth phase to kill the bacterial cells, and ultrasonic vibration is performed to rupture the bacterial cells, and 10000ml of ox bile is mixed with the bacterial strain treatment solution at a ratio of 3 to 1. Stir and keep warm at 50°C for 0.5 hours, adjust the pH value of the mixture to 3, stir and keep warm at 50°C for 0.5 hours, then add 40000ml of clarified saturated calcium oxide solution, stir well, and filter out the brown-red precipitate

[0027] (3) Add 550g bilirubin, 150g cholic acid, 75g deoxycholic acid, 6g zinc sulfate, and 6g magnesium sulfate to every 213g (dry weight) of the precipitate, stir and mix well for later use.

[0028] (4) Put the prepared compound bilirubin calcium into a low-temperature freezer, freeze at -10°C for 16 hours, then transfer it to a vacuum drying oven,...

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Abstract

The invention relates to a method for preparing composite calcium bilirubinate from ox gall. The method provided by the invention comprises the following steps of 1, adjusting taurine content of natural ox gall to 3 to 7% (g / ml), 2, inoculating a culture medium with bacterium liquid comprising one or more of escherichia coli, enterococcocci and proteus, carrying out enlargement culture until the bacteria are in a growth stationary stage, killing the bacteria, carrying out ultrasonic vibration so that the bacteria were broken, mixing the treated bacterium liquid and ox gall, and stirring at a temperature of 30 to 50 DEG C with heat preservation, 3, adjusting a pH value of the mixed solution of the ox gall and the treated bacterium liquid to 3 to 5 by a diluted acid, stirring at a temperature of 30 to 50 DEG C with heat preservation, adding a clear saturated calcium oxide solution into the mixed solution, stirring fully, filtering, and collecting brown-red precipitates, 4, adding bilirubin, cholic acid, deoxycholic acid, zinc sulfate and magnesium sulfate into the brown-red precipitates, and uniformly mixing by stirring, and 5, carrying out refrigeration and then carrying out vacuumdrying. The method provided by the invention further simplifies production processes and improves a product yield.

Description

technical field [0001] The invention belongs to the technical field of pharmacy, and relates to a method for manufacturing an intermediate of a traditional Chinese medicine raw material, in particular to a method for preparing compound bilirubin calcium by using ox bile. Background technique [0002] Bezoar is bovine gallstones, and the formation of natural bovine gallstones is the result of the joint action of many factors. The liver converts water-insoluble substances such as bilirubin and steroids into water-soluble substances by synthesizing glucuronic acid esters, and then excretes them from bile. Therefore, most of the bilirubin in bile is conjugated bilirubin. Escherichia coli in bile can produce β-glucuronidase during the growth and reproduction process, which can hydrolyze conjugated bilirubin into free bilirubin and glucuronic acid, and when glucuronic acid in bile increases, it can Prompting bacteria to produce more β-glucuronidase into bile, the increase of β-gl...

Claims

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Application Information

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IPC IPC(8): A61K35/74A61K35/413A61K31/409A61K31/575A61K33/04A61K33/08A61K33/30A61K35/62A61K35/64
CPCY02A50/30
Inventor 李幼鹤蔡红娇罗张龙
Owner WUHAN JIANMIN DAPENG PHARMA
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