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Colloidal gold assay kit for rapidly detecting dolantin and preparation of kit

A detection kit and colloidal gold technology, which is applied to measuring devices, instruments, scientific instruments, etc., can solve the problems of complex time-consuming, high cost, inability to achieve drug detection, convenient, fast and sensitive drug detection, and achieve cost saving and combination The effect of high efficiency and high sensitivity

Active Publication Date: 2014-07-16
SHANGHAI CHEMTRON BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] At present, the most widely used detection methods of pethidine are gas chromatography GC, high performance liquid chromatography HPLC, gas phase mass spectrometry GC / MS or GC / FID analysis methods. These detection methods require professional operation, and are complicated, time-consuming and expensive High, it is only suitable for the confirmation detection of pethidine, but it cannot meet the convenience, speed and sensitivity required for domestic anti-drug, anti-drug, and anti-drug operations
So far, there is no relevant patent related to pethidine rapid detection reagent in China

Method used

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  • Colloidal gold assay kit for rapidly detecting dolantin and preparation of kit
  • Colloidal gold assay kit for rapidly detecting dolantin and preparation of kit
  • Colloidal gold assay kit for rapidly detecting dolantin and preparation of kit

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0055] Example 1 Preparation of Pethidine Colloidal Gold Detection Kit

[0056] Reagent source:

[0057] Anti-demerol monoclonal antibody, goat anti-mouse IgG polyclonal antibody, and peperidine-bovine serum albumin complex (KET-BSA): purchased from MAXMED LABORATORIES INC.

[0058] Nitrocellulose film, glass fiber paper: purchased from Germany Sartorius (SARTORIUS)

[0059] Reagent preparation:

[0060] 0.1M pH7.6 phosphate buffer: weigh 10.2gNa 2 HPO 4 , 3.4gNaH 2 PO 4 , add 900ml of purified water to dissolve, adjust the pH value of the solution to 7.6 with hydrochloric acid, and dilute to 1000ml with purified water.

[0061] 10% bovine serum albumin (BSA) solution: Weigh 100 g of BSA, dissolve it in purified water, and dilute to 1000 ml.

[0062] 0.1% BSA borate buffer solution: Weigh 9.535g of borax and dissolve it in 900ml of purified water, adjust the pH value to 8.6±0.05 with hydrochloric acid, and set the volume to 1000ml to obtain 0.1M borate buffer solution. ...

Embodiment 2

[0097] The sensitivity experiment of embodiment 2 pethidine colloidal gold detection kit

[0098] Detection method:

[0099] 1. Get the pethidine colloidal gold detection kit prepared in Example 1, and place the kit on a horizontal platform.

[0100] 2. Take the sample with the sample pipette, and then drop 3 drops (about 120ul) of the sample into the sample hole of the kit. Use a different pipette for each different sample.

[0101] 3. Observation results: Interpret the results 3-8 minutes after adding the sample.

[0102] Test sample:

[0103] Quality control reference substances with peperidine concentrations of 450ng / ml, 900ng / ml, and 1800ng / ml were prepared as samples.

[0104] Test results:

[0105] Demerol concentration

[0106] The results confirmed that the sensitivity of the prepared pethidine colloidal gold detection kit met the requirements.

Embodiment 3

[0107] The clinical trial of embodiment 3 pethidine colloidal gold detection kit

[0108] Collect 50 human urine samples, including 20 urine samples of peperidine users and 30 urine samples of normal people, and use 50 peperidine colloidal gold detection kits to test the urine samples respectively. Add 2-3 drops of urine sample to the sample hole of the kit, and the kit will start to develop color after 5 minutes, and the color will be stable after 6 minutes, achieving the goal of developing color within 8 minutes. The results showed that the urine samples of 20 pethidine users were all positive, the test results of 30 normal people were all negative, and there was no uncolored kit.

[0109] The test results of gas phase mass spectrometer GC / MS are used as reference reagents to make statistics on the results of clinical trials, and the statistical data are shown in the following table:

[0110] Summary table of clinical trial results

[0111]

[0112] Gas chromatography m...

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Abstract

The invention provides a colloidal gold assay kit for rapidly detecting dolantin and preparation of the kit. The kit comprises a reagent strip sequentially provided with filter sample paper, an immunologic colloidal gold paper piece, an immunologic nitrocellulose membrane and absorbent paper from a sample adding end, wherein the immunologic colloidal gold paper piece is a glass fiber gasket coated with a colloidal gold labeled monoclonal dolantin antibody, and the immunologic nitrocellulose membrane is provided with a detecting line and a quality control line. Dolantin-carrier complex is spotted on the detecting line, and goat-anti-mouse IgG (immunoglobulin G) is spotted on the quality control line. The invention further provides a method for preparing the colloidal gold assay kit for rapidly detecting the dolantin. The kit for detecting the dolantin is high in sensitivity, simple and rapid in operation, accurate in detecting result, low in detecting cost and convenient in storage and transportation.

Description

technical field [0001] The invention relates to a colloidal gold detection kit and a preparation process, in particular to a peperidine colloidal gold detection kit and a preparation process. Background technique [0002] The scientific name of pethidine is pethidine, also known as medidine and demerol. It is a synthetic morphine substitute. Its hydrochloride is a white crystalline powder that can be dissolved in water and ethanol. It is generally made into injections, mainly For pain, cardiac asthma, visceral colic and other indications. The mechanism of action of pethidine on the human body is similar to that of morphine. Its euphoric and analgesic effects are smaller than that of morphine, but it can cause dependence after continuous use for 1-2 weeks. Once stopped, it will produce withdrawal syndrome similar to that of morphine withdrawal , mainly manifested as listlessness, general malaise, tearing, vomiting, diarrhea, insomnia, and severe cases will also cause collaps...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N33/577G01N33/531
Inventor 张国华
Owner SHANGHAI CHEMTRON BIOTECH