Medicament composition containing ginsenoside and cantharidin and application of medicament composition

A technology of ginsenosides and total ginsenosides is applied in the directions of drug combinations, medical preparations containing active ingredients, active ingredients of heterocyclic compounds, etc., which can solve problems such as clinical application limitations, reduce the incidence of adverse reactions, and improve safety. , Improve the effect of anti-tumor effect

Active Publication Date: 2012-07-04
SHANGHAI GREEN VALLEY PHARMA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Therefore, the clinical application of cantharidin and its e

Method used

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  • Medicament composition containing ginsenoside and cantharidin and application of medicament composition
  • Medicament composition containing ginsenoside and cantharidin and application of medicament composition
  • Medicament composition containing ginsenoside and cantharidin and application of medicament composition

Examples

Experimental program
Comparison scheme
Effect test

Example Embodiment

[0044] Example 1: Preparation of total ginsenosides

[0045] 20kg ginseng, cut into 1-2cm pieces, add 160L 50% ethanol for reflux extraction twice, the first 3 hours, the second 1.5 hours, combine the extracts, centrifuge at 4500rpm for 20 minutes, take the supernatant and filter it with filter paper, collect The filtrate is ready for use.

[0046] Get processed AB-8 resin 5kg, pack into the stainless steel chromatographic column that diameter is 30cm, the diameter-height ratio of resin bed is 1: 10, wash with water, filter the ginseng extract of gained with 1mL / min.cm 2 Speed ​​sample twice, static adsorption 12h. First wash 4BV with water and discard; then wash 3BV with 30% ethanol and discard; finally use 75% ethanol at 2-3mL / min.cm 2 Speed ​​elution, collect the eluate, concentrate and dry to obtain 50 g of total ginseng saponins.

Example Embodiment

[0047] Example 2: Determination of HPLC-ELSD spectrum of total ginsenosides

[0048] The total ginsenosides obtained in Example 1 were taken and subjected to HPLC-ELSD chromatogram determination. Chromatographic column: Kromasil C18 (250mm×4.6mm, 5.0μm). Mobile phase: A is acetonitrile, B is water, gradient elution program: 0~10min, 5%A~20%A; 10~35min, 20%A; 10~35min, 20%A~29%A; 36~ 70min, 29%A,; 70~100min, 47%A. Flow rate: 1 mL·min-1; column temperature: 35°C. Injection volume: 20 μL. Evaporative light scattering detector: T=40°C, P=0.35MPa, and the carrier gas is high-purity nitrogen. Test results such as figure 1 shown.

[0049] Depend on figure 1 It can be seen that the total ginsenosides prepared in Example 1 of the present invention include ginsenosides Rf, Rb 1 , Rc, Rb 2 , Rd, Rg 1 , Re, 3-O-β-D-glucopyranosyl-oleanolic acid-28-O-β-D-glucopyranoside, notoginsenoside NR 4 , 3-O-α-L-arabinofuranoose(2-1)-α-L-rhamnose(3-1)-β-D-glucopyranose-(4-1)-β-D -glucopyr...

Example Embodiment

[0052]Example 3: Preparation of Cantharidinium Extract

[0053] Weigh 20 g of cantharidin powder, pour it into a 1000 mL round-bottomed flask, add 250 mL of acetone, shake intermittently for 2 hours at 30 °C, and extract twice. The extracts were combined, concentrated under reduced pressure, washed with 95% ethanol:petroleum ether (1:1.5) until the washings were colorless to obtain a near-white crude product, then added about 5 mL of acetone, heated to dissolve, filtered while hot, and the filtrate was placed at 4°C Natural crystallization to obtain 50 mg of white transparent needle-like crystals with a yield of 0.25%. The product was detected by liquid chromatography and gas chromatography, and no impurity peak was found, indicating that the product purity was greater than 99.5%.

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Abstract

The invention relates to the field of Chinese medicine, and discloses a medicament composition containing ginsenoside and cantharidin and application of the medicament composition. The medicament composition containing the ginsenoside and the cantharidin contains the cantharidin or a Chinese medicinal material extract containing the cantharidin and the ginsenoside or a Chinese medicinal material extract containing the ginsenoside, wherein the ginsenoside or the Chinese medicinal material extract containing the ginsenoside is enough to reduce the toxic or side effect produced by the cantharidin. According to the medicament composition containing the ginsenoside and the cantharidin, the toxicity of the cantharidin on the kidney is obviously weakened, the adverse effect rate is obviously reduced, and the using safety of the cantharidin is greatly improved; and the medicament composition has the effect of improving tumor resistance, is safe in clinical use and low in toxic or side effects, and can be widely applied to preparation of medicaments for treating various cancers.

Description

technical field [0001] The invention relates to the field of traditional Chinese medicine, in particular to a pharmaceutical composition containing ginsenoside and cantharidin and its application. Background technique [0002] Mylabris, also known as Mylabris phalerata Pallas or Mylabris cichorii Linnaeus, is the dry body of Mylabris phalerata Pallas or Mylabris cichorii Linnaeus. Catch in summer and autumn, suffocate or burn to death, and dry in the sun. Mylabris is a kind of precious Chinese medicinal material. Li Shizhen, a great medical scientist in the Ming Dynasty, said in the masterpiece "Compendium of Materia Medica" that mylabris is pungent in nature, cold and poisonous. , breaking stones, blood accumulation, hurting human muscles, treating scabies, abortion, treating scrofula, clearing waterways, treating gonorrhea, treating malignant sores, fistulas, treating hernia, detoxifying boils, and treating rabies. [0003] Cantharidin mainly contains cantharidin, monote...

Claims

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Application Information

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IPC IPC(8): A61K36/258A61K35/64A61K31/704A61P35/00A61K31/34
Inventor 刘艳丽许琼明陈重刘江云何文君贾丹张苗苗杨世林
Owner SHANGHAI GREEN VALLEY PHARMA
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